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A kind of bacteria detection kit and its application

A technology for bacterial detection and kits, which is used in the determination/inspection of microorganisms, biochemical equipment and methods, and resistance to vector-borne diseases, etc., to achieve the effects of low cost, biosafety, and obvious enrichment effect.

Active Publication Date: 2022-08-09
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In response to these problems, in order to solve the problem of improving the adsorption efficiency of magnetic beads by special treatment under the existing special conditions

Method used

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  • A kind of bacteria detection kit and its application
  • A kind of bacteria detection kit and its application
  • A kind of bacteria detection kit and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Disposable urine sampler, take the urine sample containing Staphylococcus aureus and send it to the inspector within 2 hours. The solvent is 0.9% medical saline.

[0037] Detection process

[0038] 1) Dissolve 50mg of magnetic beads (purchased from Xiamen Primaige Biotechnology Co., Ltd.) in 1ml of equilibrium solution (containing 0.20g glucose, 0.10g mannose, 0.05g galactose and 0.05g maltose, histamine, sphingomyelin 0.0005g each of amino alcohol, dichlorfenuron, benzothiazole, imidazole acrylic acid, and glutamine, and 0.05g dextrin) and mix them evenly;

[0039] 2) Take 30ml of urine sample and place it in a 50ml centrifuge tube, add 3ml of balance solution (containing 0.60g of glucose, 0.30g of mannose, 0.15g of galactose and 0.15g of maltose, histamine, sphingosine, diazophos, benzoin Thiazole, imidazole acrylic acid, glutamine each 0.0015g, 0.15g dextrin) mixed evenly;

[0040] 3) Mix the mixed solution obtained in step 2) and the magnetic beads obtained in st...

Embodiment 2

[0043] Disposable urine sampler, after taking a urine sample containing Escherichia coli, send it to the inspector within 2 hours. The solvent is 0.9% medical saline.

[0044] Detection process

[0045] 1) Dissolve 50 mg of magnetic beads in 1 ml of balanced solution (containing 0.20 g glucose, 0.10 g mannose, 0.05 g galactose and 0.05 g maltose, histamine, sphingosine, diazophos, benzothiazole, imidazole acrylic acid) , 0.0005g of glutamine, 0.05g of dextrin) and mix well;

[0046] 2) Take 30ml of urine sample and place it in a 50ml centrifuge tube, add 3ml of balance solution (containing 0.60g glucose, 0.30g mannose, 0.15g galactose and 0.15g maltose, histamine, sphingosine, diazophos, benzoin Thiazole, imidazole acrylic acid, glutamine each 0.0015g, 0.15g dextrin) mixed evenly;

[0047] 3) Mix the mixed solution obtained in step 2) and the magnetic beads obtained in step 1) in a volume ratio of 1500:1, and let stand for 10 min;

[0048] 4) Place the mixed solution obtai...

Embodiment 3

[0052] Stability test

[0053] 1) Thermal stability test

[0054] The balance solution in Example 1 and the magnetic beads treated with the balance solution were placed in a water bath at 40°C in the dark for 3 days, no delamination was found with the naked eye, and no crystals were precipitated under the microscope, and then the test was carried out according to the operation steps of Example 1, It was found that the enrichment effect was not diminished.

[0055] 2) Cold stability test

[0056] The balance solution in Example 1 and the magnetic beads treated with the balance solution were placed in a -20°C refrigerator to avoid light for 1 month, during which time the freeze-thaw was repeated several times, taken out, no delamination was found with the naked eye, and no crystals were precipitated under the microscope. The operation steps of Example 1 were tested, and it was found that the enrichment effect was not weakened.

[0057] 3) Normal temperature stability test

...

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Abstract

The present application discloses a bacterial detection kit, comprising magnetic beads and a balance solution, wherein the balance solution includes glucose, mannose, galactose and maltose, and the ratio of the magnetic beads to the balance solution is 50mg-100mg: 1ml. The present application also provides a method of enriching bacteria. Using the bacterial detection kit of the present application to enrich bacteria is fast, convenient, and low in cost; the enrichment effect is obvious; repeated centrifugation is avoided.

Description

technical field [0001] The present application relates to the field of bacterial detection, in particular to a bacterial detection kit and application thereof. Background technique [0002] Clinical samples come from various sources, such as tissue fluid, blood, cerebrospinal fluid, pleural and ascites, urine, sputum, etc. Every year, due to the complex matrix of clinical samples, the complex types of pathogens, and the low content of pathogenic bacteria, the rapid detection of bacteria is difficult, resulting in missed diagnosis and untimely treatment, resulting in poor prognosis of patients. Most of the detection of bacteria is to improve the detection sensitivity by means of proliferation and culture, but because some bacteria are difficult to culture, and the culture process is likely to cause secondary pollution, and it is very time-consuming (24-48h), which greatly reduces the detection rate. The timeliness of the results, even missing the period of optimal treatment....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04
CPCC12Q1/04Y02A50/30
Inventor 沈弢张浩吕琳婷王誉雅
Owner PEKING UNIV
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