Preparation and immunochromatography application of magnetic composite nano material with double-layer quantum dot shell structure

A magnetic composite nanometer and quantum dot technology, which is applied in material excitation analysis, material analysis through optical means, nanotechnology, etc., can solve the problems of the advent of magnetic nanostructures and the lack of multi-layer quantum dot shells, etc., to achieve convenient operation, Facilitate rapid magnetic recovery and strong magnetic response

Pending Publication Date: 2020-03-17
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, only the magnetic nanostructure of the single-layer quantum dot shell has been reported, and there is no magnetic nanostructure of the multi-layer quantum dot shell.

Method used

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  • Preparation and immunochromatography application of magnetic composite nano material with double-layer quantum dot shell structure
  • Preparation and immunochromatography application of magnetic composite nano material with double-layer quantum dot shell structure
  • Preparation and immunochromatography application of magnetic composite nano material with double-layer quantum dot shell structure

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Embodiment 1

[0039] A double-layer quantum dot shell structure magnetic composite nano material prepared in the present invention, comprising Fe 3 O 4 Magnetic core, two layers of PEI sandwich and two layers of carboxylated quantum dots composed of quantum dot shell, magnetic Fe 3 O 4 The particle size is 150 nanometers, the thickness of the PEI self-assembly layer is 5nm, and the adsorbed carboxylated quantum dots (CdSe / ZnS-COOH) have a particle size of 10nm. The final particle size of the magnetic quantum dots is about 220nm, which has a good Dispersibility and magnetic responsiveness, the magnetic saturation value is 40.1emu / g.

[0040] A method for preparing the magnetic composite nano material with a double-layer quantum dot shell structure of the present embodiment, such as figure 1 As shown, including the following steps:

[0041] (1) Preparation of superparamagnetic 150 nanometer Fe 3 O 4 Magnetic core:

[0042] Take 0.54g FeCl 3 ·6H 2 O was dissolved in a mixed solution of 10 mL of ethyl...

Embodiment 2

[0056] The outermost layer of the double-layer quantum dot shell structure magnetic composite nano material provided by the present invention is a carboxylized quantum dot structure, and the surface has a large number of free carboxyl groups which can be used for antibody coupling, and the surface functionalization of the nano material can be realized very simply. The steps of modifying the surface of the double-layer quantum dot shell structure magnetic composite nano material of the present invention are as follows Image 6 As shown, including the following steps:

[0057] 1mg Fe 3 O 4 @QDs@QDs powder is dissolved in 1mL 2-(N-morpholine)ethanesulfonic acid solution (0.1M, pH5.5), and then 100μL carbodiimide solution (0.01M) and 20μL N-hydroxysuccinimide are added Amine solution (0.1M), ultrasonic reaction for 15min to activate Fe 3 O 4 @QDs@QDs surface carboxyl groups; then magnetic enrichment and recovery of Fe 3 O 4 @QDs@QDs, resuspend in 200μL PBST solution (0.01M, pH7.4); ad...

Embodiment 3

[0060] The double-layer quantum dot shell structure magnetic composite nano material provided by the present invention can be used as a multifunctional magnetic quantum dot label after surface modification with antibodies, which can effectively capture the target and quickly separate it by magnetic enrichment, and can also be used as a high-performance fluorescent nano The label is used for the detection of immunochromatographic system. In this embodiment, a double-layer magnetic quantum dot label modified with a Staphylococcus aureus antibody is combined with an immunochromatographic system to detect Staphylococcus aureus at different concentrations in a complex solution. Picture 8 This example shows the experimental flow chart of the double-layer magnetic quantum dot label used as a magnetic fluorescent label in combination with an immunochromatographic system for rapid detection of Staphylococcus aureus. Picture 9 It is based on the double-layer magnetic quantum dot immunoch...

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Abstract

The invention discloses preparation and immunochromatography application of a magnetic composite nano material with a double-layer quantum dot shell structure. The preparation method of the magnetic composite nano material with the double-layer quantum dot shell structure comprises the following steps: modifying Fe3O4 magnetic nano-particles with polyethyleneimine and adsorbing a layer of carboxylated quantum dots to form a magnetic compound Fe3O4 @QDs with a single-layer quantum dot shell; and then modifying the obtained Fe3O4 @QDs again by using PEI and then adsorbing a second layer of carboxylated quantum dots to form the magnetic composite nano material Fe3O4 @QDs@QDs with the double-layer quantum dot shell structure. The invention also discloses application of the magnetic quantum dottag prepared from the magnetic composite nano material with the double-layer quantum dot shell structure in fluorescence immunochromatography detection. The magnetic quantum dot tag is good in dispersity, high in magnetic responsiveness, adjustable in particle size, high in fluorescence intensity and easy to prepare on a large scale and can be used for capturing, enriching and high-sensitivity detection of target substances in a complex system.

Description

Technical field [0001] The invention relates to the field of novel magnetic nanomaterials and quantum dots, in particular to the preparation and immunochromatographic application of a novel double-layer quantum dot shell structure magnetic composite nanomaterial. Background technique [0002] Semiconductor quantum dots (QDs) have unique optical and chemical properties such as strong fluorescence, high light stability, size dependence, wide excitation spectrum, and long fluorescence lifetime. In recent years, they have been widely used in biological analysis and biological imaging. In recent years, quantum dots have been widely used in immunochromatographic detection systems as new fluorescent labels. These quantum dot-based fluorescent immunochromatography have achieved rapid detection of a variety of biochemical molecules. Quantum dot tags produce light-stable, strong, and readable fluorescent signals for quantitative detection of target substances, so they can be a good substit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/533G01N33/569G01N21/64B82Y40/00B82Y20/00
CPCG01N33/558G01N33/533G01N33/56938G01N21/6428B82Y40/00B82Y20/00
Inventor 王升启汪崇文肖瑞
Owner ACADEMY OF MILITARY MEDICAL SCI
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