1 results about "Measurement reproducibility" patented technology

To provide reagents and measurement methods that exhibit excellent measurement reproducibility. [Solution] A reagent for measuring a target substance contained in a sample, wherein the target substance is ferritin, and the reagent comprises a first reagent containing a buffer and a second reagent containing particles on which a ligand that specifically binds to the target substance is immobilized, wherein a first mixing step is performed to obtain a first mixed solution by mixing a sample containing 1000 ng / mL of the target substance with the first reagent in a volume ratio of sample:first reagent = 15:60, and a second mixing step is performed 280 seconds or more after mixing in the first mixing step to obtain a second mixed solution by mixing the first mixed solution and the second reagent in a volume ratio of first mixed solution:second reagent = 75:30, wherein the absorbance change of the second mixed solution becomes 0.15 or more within 50 seconds after mixing in the second mixing step.