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Method for detecting quinolone antibiotics by electrochemiluminescence sensor

A quinolone, sensor technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, etc., can solve the problems of complicated operation, expensive instruments, and the sensitivity needs to be improved.

Inactive Publication Date: 2014-04-30
周亚民
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have disadvantages such as complex operation, expensive instruments, use of toxic reagents, and sensitivity to be improved.
Electrochemiluminescence is the electroactive substance produced by direct oxidation on the electrode surface undergoes an electron transfer reaction to form an excited state, and then the excited state energy is released in the form of light. Electrochemiluminescence detection method has the advantages of high sensitivity, wide linear range, and easy operation of the instrument. , currently based on the Nafion membrane through the nano-gold and Ru(bpy) 3 2+ Fixed on the surface of the electrode and purchased as an electrochemiluminescent sensor to detect quinolone antibiotics has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The electrode is a GC electrode, the content of Nafion in Nafion ethanol solution is 0.1%, Ru(bpy) 3 2+ The content of the complex in Nafion ethanol solution is 1%, the content of nano gold in Nafion ethanol solution is 0.02%, containing Ru(bpy) 3 2+ The spreading amount of Nafion ethanol solution of the complex and gold nanoparticles on the electrode surface is 0.2ml / cm 2 , the buffer system is 0.1mol / L phosphate buffer with pH=6.5. The electrochemiluminescence intensity after adding levofloxacin was higher than that before levofloxacin was added, and the detection range of levofloxacin was 8.0×10 -6 ~8.5×10 -9 mol / L, the correlation coefficient is 0.978.

Embodiment 2

[0032] The electrode is a GC electrode, the content of Nafion in Nafion ethanol solution is 0.5%, Ru(bpy) 3 2+ The content of the complex in Nafion ethanol solution is 2%, the content of nano gold in Nafion ethanol solution is 0.1%, and contains Ru(bpy) 3 2+ The amount of Nafion ethanol solution of the complex and nano-gold spread on the electrode surface is 3ml / cm 2 , the buffer system is 0.1mol / L phosphate buffer with pH=7.2. The electrochemiluminescence intensity after adding norfloxacin was higher than that before adding norfloxacin, and the detection range of norfloxacin was 2.0×10 -5 ~6.0×10 -9 mol / L, the correlation coefficient is 0.960.

Embodiment 3

[0034] The electrode is a gold electrode, the Nafion content in the Nafion ethanol solution is 0.2%, Ru(bpy) 3 2+ The content of complex in Nafion ethanol solution is 5%, the content of nano gold in Nafion ethanol solution is 0.08%, contains Ru(bpy)3 2+ The spreading amount of Nafion ethanol solution of the complex and gold nanoparticles on the electrode surface is 0.2ml / cm 2 , the buffer system is 0.1mol / L phosphate buffer with pH=6.5. The electrochemiluminescent intensity after adding ofloxacin was higher than that before adding ofloxacin, and the detection range of ofloxacin was 1.5×10 -5 ~3.5×10 -8 mol / L, the correlation coefficient is 0.981.

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Abstract

The invention discloses a method for detecting quinolone antibiotics by an electrochemiluminescence sensor. According to the method, nanogold and Ru(bpy)[3]<2+> are fixed on the surface of a glassy carbon electrode through a Nafion membrane, a Ru(bpy)[3]<2+> complex has an oxidation reaction for oxidizing divalent Ru into trivalent Ru, the generated trivalent Ru is reduced into the divalent Ru by the quinolone antibiotics, and fluorescent light is emitted in the reduction reaction process; the Nafion membrane can generate a static attraction force to ions and especially the hydrophobic organic positive ions can be always preferentially bonded with the Nafion membrane to be fixed in the membrane; the nanogold improves the electrochemical oxidation site of Ru(bpy)[3]<2+>; the Nafion membrane also has an enrichment effect on the quinolone antibiotics in a detected sample, so that the detection sensitivity is greatly improved; the logarithm value of the change of the electrochemiluminescence intensity of a system and the logarithm value of the concentration of the quinolone antibiotics in the system before and after the addition of the quinolone antibiotics form a good linear relationship; the content of the quinolone antibiotics in the detection system can be determined through a standard curve.

Description

technical field [0001] The invention belongs to the technical field of electrochemiluminescence detection, and in particular relates to a method for detecting quinolone antibiotics by using an electrochemiluminescence sensor. Background technique [0002] Quinolones antibiotics have the advantages of good antibacterial effect, unique mechanism of action, and flexible molecular structure modification. After decades of development, it has now developed to the fourth generation, and is widely used in the treatment of various bacterial infections of the human body and organisms. The most commonly used quinolone antibiotics are: levofloxacin, norfloxacin, ofloxacin, ciprofloxacin and enrofloxacin. With the widespread use of quinolone antibiotics, their possible adverse effects and side effects have also received increasing attention. Evidence shows that the residual quinolone antibiotics in the human body and organisms will cause bacteria to develop drug resistance, thereby aff...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 周亚民
Owner 周亚民
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