Molecular imprinting polymer for roxarsone detection, and preparation method thereof

A molecular imprinting and polymer technology, applied in the field of materials chemistry, can solve the problems of weak specificity, lack of selectivity, affecting the sensitivity and accurate quantification of detection methods, and achieve strong specific recognition ability, high affinity and selection. sex, improve specificity

Inactive Publication Date: 2015-04-29
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, all current pretreatment methods for roxarsone detection are purified by traditional solid-phase extraction cartridges (such as MAX), which lack selectivity and specificity, thus affecting the sensitivity and accurate quantification of the entire detection method.

Method used

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  • Molecular imprinting polymer for roxarsone detection, and preparation method thereof
  • Molecular imprinting polymer for roxarsone detection, and preparation method thereof
  • Molecular imprinting polymer for roxarsone detection, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Weigh 0.166g (1mmoL) of ethyl p-hydroxybenzoate into a test tube, add 1mL of acetonitrile, vortex to dissolve, then add 0.434mL (4mmoL) of 2-vinylpyridine functional monomer, sonicate for 5min, and let stand for 1h , forming a prepolymer.

[0049] (2) Add 4mL (20mmoL) of ethylene glycol dimethacrylate and 60mg (0.365mmol) of azobisisobutyronitrile to the above prepolymer, sonicate for 5min, pass nitrogen gas for 5min under ice bath, seal, and vacuum at 60°C Polymerized in a dry box for 24 hours to obtain block polymer.

[0050] (3) Grind the above-mentioned massive polymer, pass through a 200-mesh sieve, and repeatedly settle with methanol to remove fine particles, put it into a 15mL solid-phase extraction column, and first use a mixed solution of acetic acid and methanol (the volume ratio of acetic acid and methanol is 1 : 9) Wash with 200mL to remove the template ethyl p-hydroxybenzoate, control the flow rate below 1mL / min, wash with 40mL of methanol to remove ac...

Embodiment 2

[0053] (1) Weigh 0.217g (1mmoL) of arsanic acid into a test tube, add 6mL of methanol-acetonitrile solution (the volume ratio of methanol to acetonitrile is 1:1), vortex to dissolve, then add 0.434mL (4mmoL) of 2-ethylene Pyridine functional monomer, ultrasonic 5min, stand for 1h to form a pre-polymer.

[0054] (2) Add 4mL (20mmoL) of ethylene glycol dimethacrylate and 60mg (0.365mmol) of azobisisobutyronitrile to the above prepolymer, sonicate for 5min, pass nitrogen gas for 5min under ice bath, seal, and vacuum at 60°C Polymerized in a dry box for 24 hours to obtain block polymer.

[0055] (3) Grind the above-mentioned massive polymer, pass through a 200-mesh sieve, and repeatedly settle with methanol to remove fine particles, put it into a 15mL solid-phase extraction column, and first use a mixed solution of acetic acid and methanol (the volume ratio of acetic acid and methanol is 1 : 9) Wash with 200mL to remove the template arsanic acid, control the flow rate below 1mL / m...

Embodiment 3

[0058] (1) Weigh 0.217g (1mmoL) of arsanic acid into a test tube, add 6mL of methanol-acetonitrile (1:1, V / V) solution, vortex to dissolve, then add 0.171mL (2mmoL) of methacrylic acid functional monomer , sonicated for 5 minutes, and allowed to stand for 1 hour to form a prepolymer.

[0059] (2) Add 3.19mL (10mmoL) of trimethylolpropane trimethacrylate and 10mg (0.06mmoL) of azobisisobutyronitrile to the above prepolymer, sonicate for 5min, blow nitrogen gas for 5min under ice bath, seal, Polymerize in a vacuum oven at 50°C for 48 hours to obtain block polymer.

[0060] (3) Grind the above-mentioned massive polymer, pass through a 200-mesh sieve, and repeatedly settle with methanol to remove fine particles, put it into a 15 mL solid-phase extraction column, and first use a mixed solution of acetic acid and methanol (1:9, V / V ) 200mL to remove the template arsanic acid, the flow rate was controlled below 1mL / min, then washed with 40mL methanol to remove acetic acid, and dried...

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Abstract

The invention belongs to the technical field of material chemistry, and discloses a molecular imprinting polymer for roxarsone detection, and a preparation method thereof. The molecular imprinting polymer preparation method comprises the following steps: carrying out polymerization of a template, a pore forming agent, a functional monomer, a cross-linking agent and an initiator for 8-48 h at a temperature of 50-80 DEG C, and removing the template to obtain the molecular imprinting polymer for roxarsone detection. According to the present invention, a roxarsone recovery rate of the prepared molecular imprinting polymer is more than 90%, and the prepared molecular imprinting polymer shows a high roxarsone cross reaction, has high selectivity and high specificity, and provides broad application prospects in the field of application of the prepared molecular imprinting polymer as a sample purification pretreatment material for roxarsone analysis in feeds, animal tissues, environment water and other matrixes.

Description

technical field [0001] The invention belongs to the technical field of material chemistry, and in particular relates to a molecularly imprinted polymer for detecting roxarsone and a preparation method thereof. Background technique [0002] Roxarsone, chemical name: 3-nitro-4-hydroxy-phenylarsine acid, and arsanilic acid (P-Arsanilic acid) are two arsenic-containing feed additives widely used in countries around the world for many years , and roxarsone is more widely used than arsanic acid. Roxarsone is the most economical multifunctional organic arsine preparation, which can promote the growth of livestock and poultry, improve the feed conversion rate, promote the deposition of livestock pigment, and increase the effect of other anticoccidial drugs on poultry; it can treat dysentery and enteritis of pigs, Increase the laying rate of laying hens. The Ministry of Agriculture of my country approved the use of the drug in 1996, and then domestic production gradually began, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F226/06C08F220/06C08F212/14C08F220/56C08F222/14C08F212/36C08J9/26C08J9/28B01J20/26B01J20/30G01N1/34
Inventor 贺利民张高奎刘雅红方炳虎杨建文王宗楠杨海翠
Owner SOUTH CHINA AGRI UNIV
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