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Protein blot (Western) A protein blot, also known as the immunoblot or Western blot, is a method of semiquantitative determination of protein expression. Crude cell lysates are loaded into a polyacrylamide gel containing a denaturing agent which give all the proteins a net negative charge.
A protein blotting device
This invention discloses a protein blotting device, belonging to the technical field of immunoblotting equipment. It includes: a base, an incubation tray placement section on the base; an incubation tray placed on the incubation tray placement section, with multiple incubation cells spaced apart on the incubation tray; a liquid addition device for respectively introducing detection solutions into the incubation cells and mounted on the base; a liquid aspiration device for respectively aspirating the detection solutions from the incubation cells and mounted on the base; and a blowing and agitating device for blowing air into the incubation cells to cause the detection solutions in the incubation cells to flow relative to the membrane strip placed in the incubation cells. The blowing and agitating device is mounted on the base and has an air outlet structure, which is located above the incubation tray placement section, with the air outlet of the air outlet structure facing the incubation cells of the incubation tray horizontally placed on the incubation tray placement section. The protein blotting device of this invention helps ensure sufficient contact and reaction between the membrane strip and the detection solution while preventing spillage of the detection solution.
Owner:GUIZHOU JINYU MEDICAL LAB CENT CO LTD