11 results about "Protein blotting" patented technology

The present invention provides a multiomics approach, which integrate single-cell RNA-sequencing (scRNA-seq) and spatiotemporal transcriptomics (ST) offering potential for dissecting transcriptional networks and revealing cell-cell interactions involved in biomolecular pathomechanisms. The present invention also provides a multimodal approach combining high-throughput scRNA-seq and ST to elucidate XLRS-specific transcriptomic signatures in two XLRS-like models with retinal splitting phenotypes, including genetically engineered (Rs1emR209C) mice and patient-derived retinal organoids harboring the same patient-specific p.R209C mutation. Through multiomics transcriptomic analysis, the endoplasmic reticulum (ER) stress / eIF2 signaling, mTOR pathway, and the regulation of eIF4 and p70S6K pathways as chronically enriched and highly conserved disease pathways between two XLRS-like models are identified. Western blots and proteomics analysis validated the occurrence of unfolded protein responses, chronic eIF2α signaling activation, and chronic ER stress-induced apoptosis. Furthermore, therapeutic targeting of the chronic ER stress / eIF2α pathway activation synergistically enhanced the efficacy of AAV mediated RS1 gene delivery, ultimately improving bipolar cell integrity, postsynaptic transmission, disorganized retinal architecture and electrophysiological responses. Collectively, the complex transcriptomic signatures obtained from Rs1emR209C mice and patient-derived retinal organoids using the multiomics approach provide opportunities to unravel potential therapeutic targets for incurable retinal diseases, such as XLRS.

This invention discloses a protein blotting device, belonging to the technical field of immunoblotting equipment. It includes: a base, an incubation tray placement section on the base; an incubation tray placed on the incubation tray placement section, with multiple incubation cells spaced apart on the incubation tray; a liquid addition device for respectively introducing detection solutions into the incubation cells and mounted on the base; a liquid aspiration device for respectively aspirating the detection solutions from the incubation cells and mounted on the base; and a blowing and agitating device for blowing air into the incubation cells to cause the detection solutions in the incubation cells to flow relative to the membrane strip placed in the incubation cells. The blowing and agitating device is mounted on the base and has an air outlet structure, which is located above the incubation tray placement section, with the air outlet of the air outlet structure facing the incubation cells of the incubation tray horizontally placed on the incubation tray placement section. The protein blotting device of this invention helps ensure sufficient contact and reaction between the membrane strip and the detection solution while preventing spillage of the detection solution.

The invention relates to the technical field of western blot membranes, in particular to a high-strength western blot membrane based on nanofiber network interpenetration and a preparation method thereof.The high-strength western blot membrane comprises the following raw materials: a polyvinylidene fluoride membrane matrix, a nanofiber skeleton, a pore-foaming agent, an N, N-dimethylacetamide cosolvent and coagulating bath; the preparation method comprises the following steps: selecting a cellulose nanofibril aqueous dispersion with the concentration of 2wt%, carrying out hydrophobic modification on the cellulose nanofibril aqueous dispersion through octyl triethoxy silane, and taking the modified cellulose nanofibril aqueous dispersion as a nanofiber skeleton; the pore-foaming agent is a compound mixed solution of polyvinylpyrrolidone and PEG-400 (Polyethylene Glycol). The cellulose nanofibrils are subjected to surface hydrophobization modification, and a compound pore-foaming agent system is introduced, so that the mechanical property and the porous structure of the membrane are considered, and the good porosity of the membrane is maintained while the overall strength of the membrane is enhanced.

Systems, methods, and components for automated processing and analyzing a biological sample are disclosed herein. Such components include pre-filled gel cards, gel card cassettes and blotting cartridges for use in automated electrophoresis and protein transfer. Systems includes automated electrophoresis and transfer modules that automatically separate proteins from the biological sample into a plurality of bands and transfer the plurality of bands to a membrane using the gel card cassettes and blotting cartridges herein. Compared to conventional methods, the disclosed system and method provide improvements in reduced experiment time, reduced error, improved precision, and reduced consumption of consumables and chemicals.

The present invention relates to the field of biotechnology and materials, in particular to an improved Western blotting membrane, its method of preparation and use. Specifically, the present invention relates to a chemically modified polyvinylidene fluoride membrane, said membrane being surface modified with a group according to Formula I: Formula I. After transfer of a protein to the chemically modified polyvinylidene fluoride membrane of the present invention, 365 nm UV light can be used to covalently crosslink the protein to the membrane. The present invention also relates to a method of preparation of the chemically modified polyvinylidene fluoride membrane, and its use in Western blotting.

This invention belongs to the field of protein antibody incubation technology and provides a protein blotting processing device, comprising: a base; a limiting post fixedly disposed on the base, one end of the limiting post having a cam component, the cam component having at least one protrusion and / or one concave portion; a turntable rotatably disposed relative to the limiting post, comprising a first fixed plate and a second fixed plate, the cam component being located between the first fixed plate and the second fixed plate, and the cam component and the second fixed plate having a preset distance; at least one first push rod movably disposed on the lower side of the second fixed plate, one end of which can abut against the cam component, the cam component being able to drive the first push rod to reciprocate along its own axis; a reaction tank movably disposed on the upper side of the second fixed plate, and passing through the second fixed plate and movably connected to the other end of the first push rod. By adopting the above structure, the reaction tank can be oscillated simultaneously with the rotation of the turntable, aided by the cam component and the first push rod.

This invention provides a polyvinylidene fluoride (PVDF) membrane, its preparation method, and its applications, relating to the field of materials technology. The preparation method of the PVDF membrane provided by this invention includes: coating a casting solution onto a substrate to form a liquid film; then, holding the carrier in an environment of 30-80°C for 1-5 seconds; subsequently, immersing the carrier in a coagulation bath to solidify it into a film; and finally, washing with water to obtain the PVDF membrane. This preparation method is environmentally friendly, simple, and low-cost. The obtained PVDF membrane has a dendritic-spherulitic alternating porous structure with controllable pore size, and can be used in biotechnology applications such as protein imprinting and sterilization filtration.

The application discloses a membrane strip incubation method for protein blotting and a detection method containing the same, and belongs to the technical field of immunoblotting. The membrane strip incubation method comprises the following steps: incubating a membrane strip by using a membrane strip incubation device, and placing the membrane strip into an incubation groove; adding buffer washing liquid and blocking liquid to the incubation groove, and blowing the liquid to flow to perform membrane blocking; adding a sample to be detected to the incubation groove, and blowing the liquid to flow to perform sample incubation; adding buffer washing liquid to the incubation groove, and blowing the liquid to flow to perform membrane washing; adding buffer washing liquid and enzyme combination to the incubation groove, and blowing the liquid to flow to perform enzyme combination; adding buffer washing liquid to the incubation groove, and blowing the liquid to flow to perform membrane washing; adding distilled water and color developing liquid to the incubation groove, shielding the membrane strip from light, and blowing the liquid to flow to perform light-proof color developing; adding distilled water to the incubation groove, and blowing the liquid to flow to perform color developing termination; and after each operation process is completed, liquid in the incubation groove is absorbed. The membrane strip incubation method ensures that the membrane strip is fully contacted with detection liquid and reacts.

A microfluidic Western blot method and system including a microfluidic western blot method for immunoassay of proteins, the method including introducing a sample including the proteins onto a chip; electrophoretically separating the proteins; binding the separated proteins to beads to form protein-attached beads, the beads being magnetic; flowing the protein-attached beads into a magnetic holding region; applying a magnetic field to the magnetic holding region to fix the protein-attached beads in place within the magnetic holding region; binding primary antibodies to target proteins on the protein-attached beads; binding secondary antibodies to the bound primary antibodies; and detecting the bound secondary antibodies.

This application provides an application of a reagent for quantitative detection of pyroglutamyl aminopeptidase in the diagnosis of rheumatoid arthritis (RA), where the reagent is a reagent for quantitative detection of pyroglutamyl aminopeptidase gene mRNA, a reagent for quantitative detection of pyroglutamyl aminopeptidase protein or a combination thereof. The quantitative detection is performed by enzyme-linked immunosorbent assay, Western blot, immunohistochemistry, a quantitative fluorescent polymerase chain reaction, mass spectrometry, or a combination thereof. A system for diagnosis of rheumatoid arthritis is also provided, including a sample collection module, a sample detection module and a data analysis module. The sample collection module is configured to collect a biological sample. The sample detection module is configured to quantitatively detect an expression level of pyroglutamyl aminopeptidase in vitro. The data analysis module is configured to compare a detection result with a diagnostic threshold, and generate a diagnosis report.

The invention provides a protein expression detection method based on an improved western blotting method, and the method comprises the following steps: carrying out pixel signal identification on a plurality of protein bands in a secondary antibody detection blotting membrane, and determining a detection limit value, background pixel intensity of a corresponding background signal in a set range of a target protein band, and a detection limit value of a target protein band; dividing the target pixel intensity by the background pixel intensity, generating a signal-to-noise ratio parameter of a target protein stripe, determining a pixel merging proportion of a pixel signal corresponding to the target protein stripe, identifying a pixel point location corresponding to the target protein stripe, performing pixel value merging on pixel points of the pixel point location corresponding to the target protein stripe according to the pixel merging proportion, and obtaining a pixel value merging result; and generating a western blot detection image of the target protein band, identifying lane size information of pixel points corresponding to the set protein band in the western blot detection image, and determining a protein expression detection result of the target protein band according to the linear relationship and the second lane size information. Therefore, the accuracy of protein quantitative detection is improved.