A kind of preparation method and adsorption device of protein blot polymer adsorbent

A technology of western blotting and adsorbent, which is applied in chemical instruments and methods, pharmaceutical equipment, and other chemical processes, and can solve problems such as unfavorable industrialization, lack of specificity of protein-binding toxins, and complicated removal process of template molecules. Strong permeability, wide applicability and low cost

Active Publication Date: 2021-03-12
JAFRON BIOMEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the adsorbents used in the above-mentioned blood purification system lack specificity for the adsorption of protein-bound toxins, or the removal process of template molecules is relatively complicated, which is not conducive to industrialization

Method used

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  • A kind of preparation method and adsorption device of protein blot polymer adsorbent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation method of the western blotting polymer adsorbent of this embodiment comprises the following steps:

[0036] (1) Mix and disperse 90mL of N-(β-aminoethyl)-γ-aminopropylmethyldimethoxysilane and 110mL tetraethoxysilane at 5°C under nitrogen;

[0037] (2) Weigh 40g with an average pore size of 5nm to 6nm, an average particle size of 500μm to 600μm, and a specific surface area of ​​600m 2 / g to 700m 2 / g of cellulose microspheres, add 80mL of 1mol / L NaOH aqueous solution and 26.67mL epichlorohydrin, stir for 4h; then add 133.33mL of 0.035mol / L ethylenediamine aqueous solution and 26.67mL of 1mol / L NaOH aqueous solution, reacted at 60°C for 3h, washed with deionized water until neutral; added 66.67mL of 25% glutaraldehyde solution, reacted for 4h, washed with deionized water until neutral; under normal pressure, soaked the microspheres Add 133.33mL of 4mol / L calcium carbonate aqueous solution, vacuumize, repeat 4 times, filter and dry;

[0038] (3) Accordi...

Embodiment 2

[0041] The preparation method of the western blotting polymer adsorbent of this embodiment comprises the following steps:

[0042] (1) Mix and disperse 50mL of N-(β-aminoethyl)-γ-aminopropylmethyldimethoxysilane and 150mL tetraethoxysilane at -5°C under nitrogen;

[0043] (2) Weigh 60g with an average pore size of 3nm to 4nm, an average particle size of 500μm to 600μm, and a specific surface area of ​​700m 2 / g to 800m 2 / g of cellulose microspheres, add 120mL of 1mol / L NaOH aqueous solution and 40mL epichlorohydrin, stir and react for 4h; then add 200mL of 0.035mol / L ethylenediamine aqueous solution and 40mL of 1mol / L NaOH aqueous solution , react at 60°C for 4h, wash with deionized water until neutral; add 100mL of 10% glutaraldehyde solution, react for 4h, wash with deionized water until neutral; under normal pressure, soak the microspheres in 150mL of 2mol / L calcium carbonate aqueous solution, vacuumize, repeat 3 times, filter and dry;

[0044] (3) The mass volume rati...

Embodiment 3

[0047] The preparation method of the western blotting polymer adsorbent of this embodiment comprises the following steps:

[0048] (1) 66.67mL of N-(β-aminoethyl)-γ-aminopropylmethyldimethoxysilane and 133.33mL of tetraethoxysilane were mixed and dispersed uniformly at 0°C under nitrogen;

[0049] (2) Weigh 80g with an average pore size of 7nm to 8nm, an average particle size of 500μm to 600μm, and a specific surface area of ​​500m 2 / g to 600m 2 / g of cellulose microspheres, add 160mL of 1mol / L NaOH aqueous solution and 53.33mL epichlorohydrin, and stir for 4h; then add 266.67mL of 0.035mol / L ethylenediamine aqueous solution and 53.33mL of 1mol / L NaOH aqueous solution, reacted at 60°C for 2h, washed with deionized water until neutral; added 133.33mL of 17% glutaraldehyde solution, reacted for 4h, washed with deionized water until neutral; under normal pressure, soaked the microspheres Add 266.67mL of 6mol / L calcium carbonate aqueous solution, vacuumize, repeat 5 times, filt...

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Abstract

The invention relates to a preparation method and an adsorption device of a protein imprinted polymer adsorbent. The preparation method comprises the following steps: step 1: mixing and dispersing siloxane monomers uniformly under conditions lower than normal temperature and nitrogen atmosphere; step 2 : amination and aldehydelation of polymer microspheres, and then loading porogen; step 3: uniformly dispersing the protein aqueous solution and the mixture obtained in step 2 in the mixture obtained in step 1, adding acid, and carrying out condensation reaction; step 4: The mixture obtained in Step 3 was filtered, washed until neutral and dried to prepare a polymer adsorbent for western blotting. The preparation method is simple to operate, and the obtained western blotting polymer adsorbent has a high adsorption rate of toxin, and is especially suitable for uremic hemoperfusion.

Description

technical field [0001] The invention relates to the field of blood purification adsorbent, in particular to a preparation method and an adsorption device of a protein imprinted polymer adsorbent especially suitable for uremic hemoperfusion. Background technique [0002] It is estimated that more than 1.2 million people worldwide suffer from end-stage renal disease (ESRD), and the number of patients is increasing at a rate of 6% to 7% per year. Uremic syndrome is directly related to patient disability and mortality. Protein-bound toxins account for approximately 24% of known uremic toxins. A large number of studies have shown that protein-bound toxins are involved in the progression of chronic renal failure (CKD), and are the basis of renal interstitial fibrosis and cardiovascular complications of CKD. For example, indoxyl sulfate (IS) and p-cresol sulfate (PCS) are involved in the pathophysiological process of renal interstitial fibrosis, atherosclerosis, and vascular calc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/26B01J20/30A61M1/38
CPCA61M1/38A61M2202/0413A61M2202/0445B01J20/268A61M2202/0085A61M2202/0021
Inventor 董凡许为康陈晓峰
Owner JAFRON BIOMEDICAL
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