Big molecular engram calcium orthophosphate/calcium alginate hybridized micro-balloon and method for preparing the same

A technology of hybrid microspheres and calcium alginate, applied in the field of material science, to achieve simple and easy preparation methods, easy control of the reaction process, and solve the effects of low mechanical strength and imprinting efficiency

Inactive Publication Date: 2009-10-28
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[Qiu Zehao, Ye Qiaoming, Preparation and application of organic / inorganic hybrid materials by sol-gel method, Guangzhou Chemistry, 2006, 31(1): 40-45] There is no material for hybridization of phosphate and alginate See related applications and reports

Method used

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  • Big molecular engram calcium orthophosphate/calcium alginate hybridized micro-balloon and method for preparing the same
  • Big molecular engram calcium orthophosphate/calcium alginate hybridized micro-balloon and method for preparing the same
  • Big molecular engram calcium orthophosphate/calcium alginate hybridized micro-balloon and method for preparing the same

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1. Preparation of bovine serum albumin surface imprinted calcium phosphate / calcium alginate hybrid microspheres, comprising the following steps:

[0034] 1) Add 0.5128g of sodium alginate and 0.15g of diammonium hydrogen phosphate solid into 20ml of deionized water, stir to fully dissolve, and obtain solution A;

[0035] 2) Measure 40ml of organic phase toluene, pour it into a beaker, add 0.8g sorbitan monooleate to it, stir for 5min to fully dissolve it; slowly pour solution A into the beaker containing the organic phase while stirring , stirred for 10 minutes to disperse the viscous solution A into microspheres;

[0036] 3) Add bovine serum albumin with a mass percentage concentration of 0.134% to 1.34% and a calcium chloride mixed aqueous solution B with a mass percentage concentration of 5% to the above mixing system under stirring for gelation reaction, and continue stirring for 45 minutes to obtain Microspheres; the protein template is eluted by the metho...

Embodiment 2

[0038] Example 2. Preparation of bovine serum albumin-embedded imprinted calcium phosphate / calcium alginate hybrid microspheres, comprising the following steps:

[0039] 1) Add 0.5128 g of sodium alginate and 0.15 g of diammonium hydrogen phosphate solid to 20 μmol / L bovine serum albumin solution dissolved in 20 ml of deionized water, stir to fully dissolve, and obtain solution A1;

[0040] 2) Measure 40ml of the organic phase composed of 20ml of chloroform and 20ml of n-hexane, pour it into a beaker, add 0.8g of sorbitan monooleate to it, stir for 5min to fully dissolve it; slowly dissolve the solution A1 under stirring Pour it into the beaker containing the organic phase, and stir for 15 minutes to disperse the viscous solution A1 into microspheres;

[0041] 3) Add calcium chloride mixed aqueous solution B1 with a mass percentage concentration of 5% to the above mixing system under stirring for gelation reaction, continue stirring for 45 minutes to obtain microspheres; use t...

Embodiment 3

[0043] Example 3. Preparation of phosphoric acid / calcium alginate hybrid microspheres imprinted on the surface of ovalbumin, comprising the following steps:

[0044] 1) Add 0.6186g of sodium alginate and 0.15g of diammonium hydrogen phosphate solid into 20ml of deionized water, stir to dissolve it fully, and obtain solution A; 15-30ml of butyl acetate, 25-50ml of castor oil;

[0045] 2) Measure 40ml of the organic phase composed of 15ml butyl acetate and 25ml castor oil, pour it into a beaker, add 0.04g ethyl cellulose to it, stir for 5min to fully dissolve it; slowly pour solution A into In the beaker containing the organic phase, stir for 10-15 minutes to disperse the viscous solution A into microspheres;

[0046] 3) Add ovalbumin with a mass percentage concentration of 0.134% to 1.34% and a calcium chloride mixed aqueous solution B with a mass percentage concentration of 5% to the above mixing system under stirring for gelation reaction, and continue stirring for 50 minutes...

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Abstract

The invention relates to a protein macromolecule imprinted calcium phosphate / calcium alginate hybrid microsphere and a preparation method thereof. The composition and weight percentage of calcium phosphate / calcium alginate hybrid microspheres for western blotting are as follows: sodium alginate 20%-40%, diammonium hydrogen phosphate 1%-10%, calcium chloride 50%-65%, Protein 0.5-10%. The calcium phosphate / calcium alginate hybrid microspheres prepared by the present invention show that the adsorption capacity of the imprinted microspheres is 1.8 to 5 times that of the non-imprinted microspheres, that is, the microspheres prepared without adding protein, according to the protein adsorption test results. Conductivity titration and infrared spectroscopy tests show that diammonium hydrogen phosphate and sodium alginate can interact with CaCl2 to produce new hybrid components. The invention has the advantages of simple preparation method, easy control of the reaction process, etc., solves the shortcomings of pure calcium alginate such as low mechanical strength and imprinting efficiency, unstable performance, difficult diffusion of protein macromolecules, and the like, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of material science, and in particular relates to a protein macromolecule imprinted calcium phosphate / calcium alginate hybrid microsphere and a preparation method thereof. Background technique [0002] At present, molecular imprinting technology has been successfully used in the imprinting of low molecular weight compounds. However, due to some characteristics of protein molecules, such as bulky size, easy denaturation due to environmental influences, complex chemical structure, and unclear recognition mechanism, the research on large molecular weight protein imprinting is difficult. It is far less systematic and in-depth than the imprinting research of small molecules. Traditionally prepared molecularly imprinted polymers are highly cross-linked and rigid materials that do not have sufficient flexibility and internal pore size to allow bulky protein templates to easily enter and exit the polymer; proteins a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L5/04C08K3/32C08J3/12C08J9/26
Inventor 成国祥赵孔银黄建军张立广
Owner TIANJIN UNIV
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