Immune colloidal gold reagent for detecting chloromycetin and preparation method thereof

A technology of chloramphenicol and colloidal gold, applied in the direction of antibodies, measuring devices, instruments, etc., can solve the problems of long detection time, complicated operation process, high detection cost, etc., and achieve easy promotion and use, simple production process and detection speed fast effect

Inactive Publication Date: 2004-11-17
博顿生物检验技术(杭州)有限公司
View PDF0 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of the enzyme-linked immunosorbent (EIA) method are: special instruments and equipment such as microplate readers are required for use; detection operators need to undergo professional training; the operation process is relatively complicated, and the time required for detection is relatively long; the cost of detection High, unable to achieve single-person detection
The disadvantages of radioimmunoassay are: professional equipment is needed, and the equipment is expensive; operators need professional training; the operation process is complicated and demanding; reagents need to be stored at low temperature; because the test involves radioactive Rarely used; high testing cost; long testing time
The disadvantages of immunoaffinity chromatography (IAC) are: it is necessary to prepare a special immunoaffinity column; operators need to undergo professional training; the operation process is complicated and demanding; the detection cost is high; the detection takes a long time; it is mainly used for sample processing , the

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0007] Adhere one end of the PVC backing to the sample pad, the binding pad in turn, the nitrocellulose membrane in the middle, and the other end to the absorbent pad. The binding pad is coated with anti-chloramphenicol specific monoclonal antibody-colloidal gold label. The nitrocellulose membrane was coated with chloramphenicol-BSA conjugate and goat (rabbit) anti-mouse IgG.

[0008] Prepare according to the following steps: (1) prepare chloramphenicol-BSA conjugate: chloramphenicol and BSA are mixed by 1: 5~50 (mol / mol) ratio, make chloramphenicol and BSA form stable particle, Form chloramphenicol-BSA conjugates by purification; (2) prepare anti-chloramphenicol-specific monoclonal antibodies: immunize Balb / c pure line mice with chloramphenicol-BSA conjugates several times, and take mouse spleen Cells and myeloma cells are fused in vitro to form hybridoma cells, and positive hybridoma cell lines can be obtained through selective medium screening. A large amount of anti-chlo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention is a kind of immune colloidal gold reagent for measuring the oxymycin and the manufacturing method. The reagent includes a sample cushion, a colloidal gold combination cushion, aeotic acid cellulose film, a suction cushion and a PVC back lining, one end of the PVC back lining is adhered with sample cushion, colloidal gold combination cushion in order, the middle part is adhered with aeotic acid cellulose film, the other end is adhered with a suction cushion. The character lies in: the colloidal gold combination cushion is enclosed with a kind of oxymycin resisting specificity single clone antibody-colloidal gold label, the aeotic acid cellulose film is enclosed by oxymycin-BSA couplings and sheep (rabbit) antiplague IgG. The reagent has a strong specificity, high sensitivity, quick and simple, cheap, and convenient.

Description

technical field [0001] The invention relates to an immune colloidal gold reagent for detecting antibodies, and also relates to a preparation method of the reagent. Background technique [0002] Existing methods for detecting chloramphenicol mainly include enzyme-linked immunosorbent assay (EIA), radioimmunoassay (RIA), immunoaffinity chromatography (IAC), and gas / mass spectrometry. The disadvantages of the enzyme-linked immunosorbent (EIA) method are: special instruments and equipment such as microplate readers are required for use; detection operators need to undergo professional training; the operation process is relatively complicated, and the time required for detection is relatively long; the cost of detection Higher, single-person detection cannot be realized. The disadvantages of radioimmunoassay are: professional equipment is needed, and the equipment is expensive; operators need professional training; the operation process is complicated and demanding; reagents nee...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K39/395G01N33/532G01N33/558G01N33/569
Inventor 张少恩
Owner 博顿生物检验技术(杭州)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap