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Pretreatment method for detecting chloromycetin in animal product

An animal product, chloramphenicol technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of many pretreatment steps, different antibody batches, and different determination results, etc., to achieve low operating costs and low detection limits. , the effect of reducing the cost of testing

Inactive Publication Date: 2015-01-14
CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microbiological method is easy to operate, low cost, but low sensitivity and poor specificity; immunoassay has the advantages of high sensitivity, strong specificity, low requirements for equipment and personnel quality, and simple sample pretreatment, which is suitable for on-site monitoring and large-scale sample screening , but there are many influencing factors, false positive results are prone to occur, different antibody batches, and the measurement results will also vary. Therefore, there is an urgent need for a pretreatment method with low operating cost, high reproducibility, high accuracy, and high sensitivity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A pretreatment method for detection of chloramphenicol in animal products, consisting of the following steps:

[0019] (1) Extraction: Weigh 5.0g of homogenized meat or eggs into a 100mL centrifuge tube, add 20mL of ethyl acetate, homogenize for 1min, vortex for 20min, and centrifuge at 3000r / min for 5min; take the supernatant and spin Put it into another 100mL centrifuge tube, use 15mL ethyl acetate to extract the sample again, combine the supernatant, and blow dry with nitrogen;

[0020] (2) Purification: add 1 mL of methanol to dissolve the residue by ultrasonication, then add 20 mL of 5% sodium chloride and 10 mL of n-hexane in turn; vortex and shake for 10 min. Discard the n-hexane layer; add 15 mL of ethyl acetate, vortex and shake for 10 minutes, let stand to separate layers, absorb the upper layer and transfer it to a 15 mL centrifuge tube with a stopper, and blow nitrogen to 1 mL;

[0021] (3) Derivatization: transfer the above liquid into a 5 mL stoppered cen...

Embodiment 2

[0023] A pretreatment method for detection of chloramphenicol in animal products, consisting of the following steps:

[0024] (1) Extraction: Weigh 3g of homogenized meat or eggs into a 100mL centrifuge tube, add 25mL of ethyl acetate, homogenize for 0.5min, vortex for 25min, and centrifuge at 2500r / min for 8min; take the supernatant and spin Put it into another 100mL centrifuge tube, use 10mL ethyl acetate to extract the sample again, combine the supernatant, and blow dry with nitrogen;

[0025] (2) Purification; add 1.5 mL of methanol to dissolve the residue by ultrasonication, then add 15 mL of 8% sodium chloride and 5 mL of n-hexane in sequence; vortex and shake for 15 minutes, discard the n-hexane layer; then add 10 mL of ethyl acetate, vortex and shake for 15 minutes, and Set the layers, absorb the upper layer and transfer it to a 15mL centrifuge tube with a stopper, blow with nitrogen to 0.5mL;

[0026] (3) Derivatization: transfer the above liquid into a 5 mL stoppere...

Embodiment 3

[0028] A pretreatment method for detection of chloramphenicol in animal products, consisting of the following steps:

[0029] (1) Extraction: Weigh 8g of homogenized meat or eggs into a 100mL centrifuge tube, add 15mL of ethyl acetate, homogenize for 1.5min, vortex for 15min, and centrifuge at 3500r / min for 3min; take the supernatant and spin Put it into another 100mL centrifuge tube, use 20mL ethyl acetate to extract the sample again, combine the supernatant, and blow dry with nitrogen;

[0030] (2) Purification; add 0.5mL methanol to ultrasonically dissolve the residue, add 25mL sodium chloride with a concentration of 3% and 15mL n-hexane in turn; vortex and shake for 5min, discard the n-hexane layer; then add 20mL ethyl acetate, vortex and shake for 5min, static Set the layers, absorb the upper layer and transfer it to a 15mL centrifuge tube with a stopper, blow with nitrogen to 1.5mL;

[0031] (3) Derivatization; transfer the above liquid into a 5 mL stoppered centrifuge ...

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Abstract

The invention belongs to the technical field of food safety, and particularly relates to a pretreatment method for detecting chloromycetin in an animal product. The pretreatment method disclosed by the invention comprises the following operations: weighing of a to-be-detected sample, extraction, cyclotron oscillation, centrifugation, purification, derivatization and the like, wherein the used apparatus comprises a centrifugal machine, an oscillator, a constant-temperature drying oven, a pressure blowing concentrator and the like. A sample solution prepared by using the pretreatment method can be directly detected by virtue of a gas chromatography-mass spectrometry method. Compared with an existing method, the pretreatment method has the advantages of being low in operation cost, high in repeatability, high in accuracy, high in sensitivity and the like, has strong operability, is expected to be popularized and applied on a large scale in the industries such as agriculture, quality inspection departments, and entry and exit inspection and quarantine, and has significant economic benefits. According to the pretreatment method, whether chloromycetin is contained in meat can be accurately detected, and the detected animal product is safe to eat and accords with the requirements of the national standard in China.

Description

technical field [0001] The invention belongs to the technical field of food safety, and in particular relates to a pretreatment method for detecting chloramphenicol in animal products. Background technique [0002] Chloramphenicol is an efficient and broad-spectrum antibiotic, which has been widely used in the prevention and treatment of various animal infectious diseases. Due to the serious side effects of chloramphenicol, long-term trace intake of chloramphenicol will not only cause Escherichia coli , Salmonella and other strains produce drug resistance, and will cause the normal flora imbalance of animals, lower resistance, and susceptibility to various diseases. The residues of chloramphenicol in food animals can be enriched in the human body through the food chain, thus posing a potential hazard to human health. Many countries and regions have successively banned or strictly restricted the use of chloramphenicol. On September 13, 1999, the Ministry of Agriculture of my...

Claims

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Application Information

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IPC IPC(8): G01N30/06
Inventor 路平
Owner CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENT
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