Mouse antichloromycetin monoclonal antibody and its use

A monoclonal antibody, chloramphenicol technology, applied in the field of detection, can solve problems such as poor specificity

Inactive Publication Date: 2003-07-16
上海中科伍佰豪生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But polyclonal antibody enzyme-linked immunosorbent technique sensitivity, specificity are relatively poor, therefore, this area urgen

Method used

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  • Mouse antichloromycetin monoclonal antibody and its use

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Experimental program
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Embodiment 1

[0037] Preparation and purification of anti-chloramphenicol monoclonal antibody

[0038] (A) Immunization antigen, detection antigen, preparation of chloramphenicol enzyme marker:

[0039] According to the method described in Domenici.C.et al.Anti-cap antibodies for application to a tirf immunosensor.Life chemistry Reports.1994, Vol 11 P 409, chloramphenicol hemisuccinate was mixed with BSA, rabbit IgG, and HRP respectively. The carbodiimide-activated lipid method was used for coupling to prepare CAP-HS-BSA as an immune antigen, CAP-HS-IgG as a detection antigen, and CAP-HS-HRP as a chloramphenicol enzyme marker.

[0040] (B) Establishment of anti-CAP hybridoma cell lines

[0041] Each mouse was injected intraperitoneally and at the nape of the neck with 50 μg of antigen emulsified for a total of 8 times, and then the splenocytes of the mice were fused with SP2 / 0. The serum titer of immunized BALB / c mice reached 1:15000, and the cell fusion rate was over 95%. A total of 46 p...

Embodiment 2

[0045] Characterization of Monoclonal Antibodies

[0046] (A) Subtype identification

[0047] The conventional double-sandwich ELISA method was used for subtype screening and identification.

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Abstract

A monoclonal antibody GA3 of chloromycetin for mouse is disclosed, which has strong specificity for binding with chloromycetin. The immunoglobulin 6A3, its fragment and immune conjugate, and a reagent kit for detecting the residue of chloromycetin are also disclosed.

Description

technical field [0001] The present invention relates to the field of detection. More specifically, the present invention relates to specific monoclonal antibodies against chloramphenicol and applications thereof. Background technique [0002] Chloramphenicol is a broad-spectrum antibiotic widely used in clinical bacterial disease control. Because of its toxic and side effects on the human hematopoietic system, especially the bone marrow suppression in children, it has been strictly controlled for clinical treatment. [0003] Due to the effectiveness and cheapness of chloramphenicol, it is still used to control various animal diseases. However, it will remain in animal tissues and secretions (for example: meat, eggs, honey, milk), causing people to ingest chloramphenicol indirectly through the food chain, endangering human health. (Domenici.C.et a1.Anti-cap antibodies for application to a tirf immunosensor.Lifechemistry Reports.1994, Vol 11 P 409; Everest, S.J.et al.Develo...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N5/18G01N33/68
Inventor 魏万贵陈登鸿罗荣生金玫蕾胡泰山赵国屏
Owner 上海中科伍佰豪生物工程有限公司
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