Preparation method of chloramphenicol molecular imprinted adsorbing material on surface of magnetic carbon microsphere

An adsorption material and molecular imprinting technology, which is applied in the direction of analytical materials, selective adsorption, chemical instruments and methods, etc., to achieve high selectivity, low cost and fast adsorption speed

Active Publication Date: 2015-07-01
HENAN UNIV OF URBAN CONSTR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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This preparation method

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  • Preparation method of chloramphenicol molecular imprinted adsorbing material on surface of magnetic carbon microsphere
  • Preparation method of chloramphenicol molecular imprinted adsorbing material on surface of magnetic carbon microsphere
  • Preparation method of chloramphenicol molecular imprinted adsorbing material on surface of magnetic carbon microsphere

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of magnetic carbon microspheres

[0025] Take the pomelo peel that has been cleaned of impurities on the inner and outer surfaces, cut it into small sections with a length and width of 2-10 cm, and dry it at 100°C for 24 hours to obtain dried grapefruit peel; press ferric nitrate nonahydrate, the dried grapefruit peel prepared above and ethanol Mix at a ratio of 1:1.5:200 (g / g / mL), ultrasonically disperse evenly, stir magnetically at room temperature for 20 hours, and dry in an oven at 60°C; then soak in propionic acid vapor at 80°C for 16 hours; then put the mixture In a tube furnace under a nitrogen atmosphere, the temperature was raised to 500°C at 5.0°C / min, and the temperature was maintained for 2.0h for calcination. The calcined product was washed with ethanol several times, and dried to constant weight to obtain magnetic carbon microspheres.

[0026] (2) Vinyl modification on the surface of magnetic carbon microspheres

[0027] According to magne...

Embodiment 2

[0034] Take 25ml of CAP solutions with initial concentrations of 5, 10, 20, 30, 50, 60, 80, 100, 150, and 200 μmol / L, respectively, and add them to centrifuge tubes, and add 10 mg of molecularly imprinted adsorption materials on the surface of magnetic carbon microspheres and non- Imprint the adsorption material, put the test solution in a 25°C water bath and let it stand for 12.0h, separate and collect the supernatant with a Nd-Fe-B permanent magnet, and measure the molecular concentration of unadsorbed chloramphenicol with a UV-visible spectrophotometer , to calculate the adsorption capacity, figure 2 The results show that the imprinted adsorption material has a saturated adsorption capacity of 51.85umol / g, which is higher than that of the non-imprinted adsorption material, which is 25.20umol / g, which proves that the imprinted adsorbent has a large number of imprinted holes and shows a good imprinting effect.

Embodiment 3

[0036] Take 25ml of CAP solution with an initial concentration of 100μmol / L and add it to a centrifuge tube, add 10mg of magnetic carbon microsphere surface molecularly imprinted adsorption material and non-imprinted adsorbent material, and place the test solution in a water bath at 25°C for 2 and 5 minutes respectively. , 10, 30, 45, 60, 90, 120 and 150min, after standing still, the supernatant was separated and collected by Nd-Fe-B permanent magnet, and the molecular concentration of unadsorbed chloramphenicol was measured by UV-visible spectrophotometer , to calculate the adsorption capacity from image 3 It can be seen that at the beginning, with the increase of adsorption time, the adsorption capacity increased rapidly, and the adsorption reached equilibrium after 30 min. In the whole adsorption time range, the adsorption capacity of magnetic molecularly imprinted adsorbents for chloramphenicol molecules was greater than that of non-imprinted adsorbents for chlorine Mycin...

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Abstract

The invention relates to a preparation method of a chloramphenicol molecular imprinted adsorbing material on the surface of a magnetic carbon microsphere. The magnetic carbon microsphere is prepared from shaddock peel by adopting a solvent thermal synthesis method, the surface of the magnetic carbon microsphere is subjected to ethylene functional modification by KH570, the treated magnetic carbon microsphere is used as a matrix material, chloramphenicol is used as a template, hydroxyethyl methylacrylate and 4-vinylpyridine are used as functional monomers, N-isopropyl acrylamide is used as a thermosensitive monomer, ethylene glycol dimethacrylate and N,N-methylene bisacrylamide are used as cross-linking agents, 2,2'-azobis[2-methylpropionamidine] dihydrochloride is used as an initiator, and the magnetic carbon microsphere surface imprinted thermosensitive adsorbing material is prepared in a methanol/water mixed system, and the adsorbing material is used for selective recognition and selective separation of chloramphenicol in a water environment. The prepared molecular imprinted adsorbing material on the surface of the magnetic carbon microsphere is low in cost, can be prepared easily, has the high recognition and selectivity properties, high separation and enrichment ability and high adsorption rate of target molecules, and is high in adsorption speed.

Description

technical field [0001] The invention relates to a preparation method of a chloramphenicol molecularly imprinted adsorption material on the surface of magnetic carbon microspheres, and belongs to the technical field of preparation of environmental functional materials. Background technique [0002] Chloramphenicols (CAPs) are broad-spectrum antibiotics of amide alcohols, which have strong inhibitory effects on Gram-negative bacteria and Gram-positive bacteria, and have fast absorption, good curative effect, and broad-spectrum antibacterial properties. And other advantages, it is widely used in intestinal, urinary tract and respiratory diseases caused by sensitive bacteria of poultry and livestock. Drug abuse and illegal use have caused drug residues in meat and aquatic products. At present, many countries, including China, have frequent detection of CAP in sewage treatment plant effluent, surface, and underground drinking water. CAP has serious toxic side effects and long-te...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/30B01J20/28B01D15/08G01N33/553
Inventor 毛艳丽康海彦罗世田王现丽吴迪梁峰朱慧杰王国贞王远红肖晓存
Owner HENAN UNIV OF URBAN CONSTR
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