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Citrobacter farmeri Citrobacter farmeriSC01 and application thereof

A technology of citric acid and bacillus, applied in the direction of bacteria, chemical instruments and methods, methods based on microorganisms, etc.

Inactive Publication Date: 2008-08-13
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is generally believed that the traditional biological treatment method is only suitable for the concentration of phenolic compounds below 500mg / L

Method used

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  • Citrobacter farmeri Citrobacter farmeriSC01 and application thereof
  • Citrobacter farmeri Citrobacter farmeriSC01 and application thereof
  • Citrobacter farmeri Citrobacter farmeriSC01 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Separation and Purification of Efficiently Degrading Phenols Strains

[0036] Take the sludge from the primary aerobic pool of the waste water treatment station of Shaogang Coking Plant in Guangdong, put the sludge in a triangular flask, aerate without adding nutrients for 48 hours, let it settle, filter, take the supernatant and insert it into phenol-degrading bacteria Selection medium (selection medium formula is: K 2 HPO 4 2.24g / L, KH 2 PO 4 2.75g / L, (NH 4 ) 2 SO 4 1g / L, MgCl 2 ·6H 2 O 0.2g / L, NaCl 0.1mg / L, FeCl 3 ·6H 2 O 0.02g / L, CaCl 2 0.01mg / L, adjust the pH value of the medium to 6.8-7.0, and sterilize at 121°C for 30 minutes), add 100mg / L phenol and 20mg / L m-cresol as carbon sources, and place in a constant temperature shaker at 30°C, Shaking culture at 130 rpm, stop the shaking culture after the solution is turbid, reconnect the culture medium with fresh selection medium, and increase the concentration of phenol in it appropriately, so t...

Embodiment 2

[0038] The property of embodiment 2 bacterial strain SC01

[0039] The pure culture m-cresol efficient degrading bacterium SC01 obtained by the separation and purification of Example 1 is identified in various properties, and the results are shown in Table 1:

[0040] Table 1 Morphological observation and physiological and biochemical experiment results of bacterial strain SC01

[0041] serial number

Test content

result

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Morphological observation

a

Catalase

Oxidase

anaerobic growth

glucose oxidative fermentation

V-P determination

Nitrate reduction

starch hydrolysis

gelatin liquefaction

Sugar and Alcohol Fermentation

Phenylalanine deaminase

citrate utilization

Lecithinase

indole

Methyl Red (MR)

...

Embodiment 3

[0044] The 16S rDNA sequence determination of embodiment 3 bacterial strain SC01

[0045] Pick the above-mentioned purely cultured high-efficiency m-cresol-degrading bacteria SC01 and put it into about 20 μL of sterile double-distilled water. After 5 minutes of boiling water bath, it will be used as a PCR template to amplify 16S rDNA to 1.5 kb. The PCR amplification reaction system is 50 μL:

[0046] Sterile double distilled water 36.5μL

[0047] 10×PCR buffer 5.0μL

[0048] 2.5mmol·L -1 dNTP5.0μL

[0049] 10μmol·L -1 F27 1.0 μL

[0050] 10μmol·L -1 R1522 1.0μL

[0051] 5U (μL) -1 Blend-Taq 0.5 μL

[0052] Template DNA 1.0 μL

[0053] The PCR reaction program is shown in Figure 1.

[0054] The amplified product was sequenced and identified, and the sequencing result is shown in SEQ ID NO: 1. According to GenBank comparison, the strain SC01 has the highest homology with Citrobacter farmeri, reaching 99%.

[0055] Based on the morphological observation, physiological ...

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PUM

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Abstract

The invention discloses a fashi citric acid rod Citrobacter farmeri SC01 and the application. The bacterium is obtained by artificial accumulation culture and separation cleansing from phenols cyanogen waste water treatment station one level aerobic pool sludge. The bacterium is preserved in china typical culture entreasure center of Wuhan University on January 7th 2008 year whose short form is CCTCC and preserving identification serial number is CCTCC NO: M208004. The fashi citric acid rod Citrobacter farmeri SC01 incorporating the invention can degrade phenyl hydrate, 2-methylphenol, m-cresol, 1-naphthyl hydroxide, 696mg / L m-cresol in 114 hours on aerobic condition, and the average degrading rate of speed ran up to 8.94mg / (L h) and the bacterium is sensitive to acheomycin, chloramphenicol, rifampicin and streptomycin, and the bacterium can be used safe and wide in harnessing and repairing wasted water and soil phenols pollution because the bacterium can not carryover drug-resistance factor spreading in aborigines bacterium when the bacterium is released into environment.

Description

technical field [0001] The invention relates to a Citrobacter farmeri SC01 capable of efficiently degrading phenols and an application thereof. Background technique [0002] Phenolic compounds are composed of a series of phenols and their derivatives, and those with a boiling point <230°C are called volatile phenols. After entering the water body, phenol seriously affects the quality of surface water and causes the death of aquatic organisms. Drinking water is contaminated with phenols, which alter the smell and taste. Phenol manufacturing, coking, oil refining, metallurgy, plastics, chemical fibers, insulating materials, phenolic resins, pharmaceuticals, explosives, pesticides and other industries will have a high concentration of phenol-containing wastewater. [0003] Phenolic compounds are a kind of protoplasmic poisons, which have a poisonous effect on all living individuals and can coagulate proteins, so they have a strong bactericidal effect. Phenolic substances ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/34
Inventor 任源韦朝海
Owner SOUTH CHINA UNIV OF TECH
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