Detection method of multiresidue of 5 nitrofuran metabolites and chloramphenicol in shrimp

A technology of nitrofuran and detection method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as toxic and side effects, and achieve the effects of improving work efficiency, improving extraction efficiency, and shortening working hours
CN106124653AActive Publication Date: 2016-11-16青岛菲优特检测有限公司
6 Cites 24 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
青岛菲优特检测有限公司
Publication Date
2016-11-16

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

A detection method of a nitrofuran metabolites and chloramphenicol multiresidue in shrimp belongs to the technical field of aquatic products detection. The method is as below: hydrolyzing a sample with hydrochloric acid, subjecting nitrofuran metabolites to derivatization by using 2-nitrobenzaldehyde, adjusting the pH value to 6.5-7.5, adding acetonitrile, adding an extraction salt package; conducting liquid-liquid extraction of a target compound by using acetonitrile; purifying and concentrating a supernatant; then determining by a liquid chromatography-tandem mass spectrometer, and quantifying by an internal standard method. The method uses a novel sample extraction and purification mode for simultaneous analysis and determination of two prohibited drugs with the highest detection rate in shrimp, overcomes the limitations of separate determination of two drugs in the detection method of the prior art, improves the extraction efficiency of chloramphenicol in actual positive samples, greatly improves the work efficiency, shortens the work time and saves reagent consumption and labor costs. The method adopts the isotope internal standard method for quantification, the determination result is more accurate and reliable, has high sensitivity, and good reproducibility and quantitative accuracy.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of aquatic product detection, and relates to a method for detecting five kinds of nitrofuran metabolites and multiple residues of chloramphenicol in shrimp by using high performance liquid chromatography-tandem mass spectrometry (LC / -MS / MS). Background technique

[0002] Nitrofuran drugs are a class of synthetic broad-spectrum antibacterial drugs with 5-nitro structure, mainly including furazolidone, furaltadone, nitrofurazone, nitrofurantoin and nifursol, etc., which have been widely used in the treatment and prevention of Gastrointestinal diseases of livestock, poultry and aquatic animals caused by Escherichia and Salmonella. The half-life of nitrofuran drugs in animals is short, but their metabolites can be tightly combined with proteins and remain for a long time. Studies have shown that nitrofuran drugs and their metabolites can induce genetic mutations in organisms, especially their metabolites are hi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More