Detection method of multiresidue of 5 nitrofuran metabolites and chloramphenicol in shrimp

A technology of nitrofuran and detection method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as toxic and side effects, and achieve the effects of improving work efficiency, improving extraction efficiency, and shortening working hours

Active Publication Date: 2016-11-16
青岛菲优特检测有限公司
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Problems solved by technology

Chloramphenicol (Chloramphenicol, CAP) is also called levomycin, which has broad-spectrum antibacterial ability and is widely used in aquaculture. I

Method used

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  • Detection method of multiresidue of 5 nitrofuran metabolites and chloramphenicol in shrimp
  • Detection method of multiresidue of 5 nitrofuran metabolites and chloramphenicol in shrimp
  • Detection method of multiresidue of 5 nitrofuran metabolites and chloramphenicol in shrimp

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Embodiment 1

[0063] Determination of nitrofuran metabolites and chloramphenicol residues in Example 1 Penaeus vannamei

[0064] 1. Instruments and equipment used in this embodiment: LC-MS / MS liquid chromatography-tandem mass spectrometer

[0065] (1) HPLC part (Shimadzu Corporation, model LC-20A), using Kinetex EVO C 18 Reverse-phase chromatographic column, 100mm×2.1mm, 2.6μm.

[0066] (2) AB Qtrap 5500 mass spectrometer (AB company in the United States): equipped with ESI ion source, triple quadrupole, collision cell, vacuum system, and gas circuit control system;

[0067] (3) Ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd., model KQ-600DE);

[0068] (4) high-speed centrifuge: 8000r / min (Thermo Fisher company);

[0069] (5) Vortex mixer (U.S. Talboys company);

[0070] (6) Nitrogen blowing instrument (Organomation Company of the United States, model N-EVAP 112);

[0071] (7) Small high-speed centrifuge: 14000r / min (Germany Sigma);

[0072] (8) Analytical balance: Sensiti...

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Abstract

A detection method of a nitrofuran metabolites and chloramphenicol multiresidue in shrimp belongs to the technical field of aquatic products detection. The method is as below: hydrolyzing a sample with hydrochloric acid, subjecting nitrofuran metabolites to derivatization by using 2-nitrobenzaldehyde, adjusting the pH value to 6.5-7.5, adding acetonitrile, adding an extraction salt package; conducting liquid-liquid extraction of a target compound by using acetonitrile; purifying and concentrating a supernatant; then determining by a liquid chromatography-tandem mass spectrometer, and quantifying by an internal standard method. The method uses a novel sample extraction and purification mode for simultaneous analysis and determination of two prohibited drugs with the highest detection rate in shrimp, overcomes the limitations of separate determination of two drugs in the detection method of the prior art, improves the extraction efficiency of chloramphenicol in actual positive samples, greatly improves the work efficiency, shortens the work time and saves reagent consumption and labor costs. The method adopts the isotope internal standard method for quantification, the determination result is more accurate and reliable, has high sensitivity, and good reproducibility and quantitative accuracy.

Description

technical field [0001] The invention belongs to the technical field of aquatic product detection, and relates to a method for detecting five kinds of nitrofuran metabolites and multiple residues of chloramphenicol in shrimp by using high performance liquid chromatography-tandem mass spectrometry (LC / -MS / MS). Background technique [0002] Nitrofuran drugs are a class of synthetic broad-spectrum antibacterial drugs with 5-nitro structure, mainly including furazolidone, furaltadone, nitrofurazone, nitrofurantoin and nifursol, etc., which have been widely used in the treatment and prevention of Gastrointestinal diseases of livestock, poultry and aquatic animals caused by Escherichia and Salmonella. The half-life of nitrofuran drugs in animals is short, but their metabolites can be tightly combined with proteins and remain for a long time. Studies have shown that nitrofuran drugs and their metabolites can induce genetic mutations in organisms, especially their metabolites are hi...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/14
CPCG01N30/02G01N30/14G01N2030/027
Inventor 邢丽红孙伟红付树林李兆新郭萌萌孙晓杰郑关超翟毓秀
Owner 青岛菲优特检测有限公司
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