Verifying test paper of chloromycetin gel by gold method and production thereof

A technology for detecting test strips and chloramphenicol, which is applied in the direction of biological testing, measuring devices, and material inspection products. Effects that are easy and cheap to make

Inactive Publication Date: 2006-08-16
YUNNAN UNIV +2
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Problems solved by technology

At present, the method for measuring chloramphenicol mainly contains methods such as high performance liquid chromatography (HPLC) and enzyme-linked immunosorbent immunoassay, and HPLC method requires special instruments and equipment and professional technicians, and sample screening amount is few, needs the pretreatment of sample, therefore Its detection cost is high and the detection time is long. These factors limit the development of HPLC detection items for chloramphenicol, and cannot fully meet the current food safety needs for detection of chloramphenicol residues.
However, enzyme-linked immunosorbent reagents mainly rely on imports, and the detection cost is relatively high.

Method used

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  • Verifying test paper of chloromycetin gel by gold method and production thereof
  • Verifying test paper of chloromycetin gel by gold method and production thereof
  • Verifying test paper of chloromycetin gel by gold method and production thereof

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Embodiment Construction

[0026] Further description will be made below in conjunction with the accompanying drawings.

[0027] 1. Chloramphenicol test paper

[0028] Including lower absorbent pad 1, gold-labeled anti-chloramphenicol monoclonal antibody 2, chloramphenicol-conjugated antigen 3, anti-mouse IgG antibody 4, upper absorbent pad 5, nitrocellulose membrane 6, and bottom plate 7.

[0029] Among them, chloramphenicol-conjugated antigen 3 was used as the detection band, and anti-mouse IgG antibody 4 was used as the reference band.

[0030] The width of the film strip is not less than 2.5mm, and the appearance is flat, the material is firmly attached, and the liquid migration speed is not less than 5mm / min.

[0031] 2. Preparation of chloramphenicol test paper

[0032] Combining chloramphenicol and bovine serum albumin molecules to form chloramphenicol-coupled antigen 3, the clone of mouse cells immunized with antigen 3 was prepared in the form of mouse ascites, and the antibody titer was 15,00...

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Abstract

A method for preparing test paper of chloromycetin colloidal gold detection technique includes anchoring chloromycetin single resistance being labeled with colloidal gold and chloromycetin coupled antigen as well as resisting mouse IgG antibody on nitrocellulose film and on base plate, using chloromycetin coupled antigen as detectionbelt and using resisting mouse IgG antibody as reference belt .The prepared test paper can detect out residual amount of chloromycetin in serum sample or in urine sample quickly and accurately.

Description

Technical field [0001] The invention belongs to a measuring test paper and a preparation method for detecting a test substance by a biological immunological method. Background technique [0002] Chloramphenicol is a class of antimicrobial drugs with broad-spectrum antibacterial activity that inhibits bacterial protein synthesis. Due to its good antibacterial and pharmacokinetic properties, it is widely used as a feed additive and therapeutic drug for bacterial diseases. Chloramphenicol is highly toxic to bone marrow and newborns, and can easily cause blood poisoning in humans, leading to irreversible aplastic anemia. Long-term intake of chloramphenicol residues in animal-derived foods such as animal muscle, viscera, milk, etc. will cause chronic or cumulative toxicity. Therefore, it directly or indirectly threatens the health of consumers and is listed in the Ministry of Agriculture " "List of Veterinary Drugs and Other Compounds Prohibited in Food A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/544G01N33/558G01N33/52
Inventor 马岚袁航葛长荣张曦
Owner YUNNAN UNIV
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