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Magnetic separation-signal amplification integrated chloramphenicol detection biosensor and method

A biosensor and signal amplification technology, applied in the field of biosensors for antibiotic detection, can solve problems such as increased cost, interference factors, and cumbersome detection steps.

Inactive Publication Date: 2017-08-11
ZHEJIANG UNIV
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  • Claims
  • Application Information

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Problems solved by technology

However, magnetic nanoparticles generally do not participate in signal output after performing the functions of separation and enrichment, resulting in the need to introduce additional signal markers, thereby increasing costs and interference factors, and making detection steps cumbersome

Method used

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  • Magnetic separation-signal amplification integrated chloramphenicol detection biosensor and method
  • Magnetic separation-signal amplification integrated chloramphenicol detection biosensor and method
  • Magnetic separation-signal amplification integrated chloramphenicol detection biosensor and method

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Embodiment Construction

[0068] The present invention will be further described below in conjunction with embodiment, but the present invention is not limited by embodiment.

[0069] Embodiments of the present invention are as follows:

[0070] 1) Preparation of gold electrodes labeled with aptamer-modified magnetic nanoparticles

[0071] 1.1) Preparation of aqueous magnetic nanoparticles (MNPs) by co-precipitation chemical synthesis method:

[0072] 1.1.1) FeCl 3 and FeSO 4 Dissolve in pH 3 hydrochloric acid diluent and continue to fill with nitrogen for 20min to remove oxygen, meanwhile prepare NaOH solution and continue to fill with argon for 20min to remove oxygen; FeCl 3 and FeSO 4 The ratio of the amount of the substance is 2:1, and the concentration of the substance in the NaOH solution is 1.25M.

[0073] 1.1.2) the above step 1.1.1) containing FeCl 3 and FeSO 4 The mixed solution was mixed rapidly with NaOH solution, continuously stirred, and filled with argon for 2 hours to obtain ferr...

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Abstract

The invention discloses a magnetic separation-signal amplification integrated chloramphenicol detection biosensor and method. Chloramphenicol aptamer modified magnetic nanoparticles are combined with chloramphenicol in a sample, rapid separation and enrichment are achieved through magnetic separation operation, one section of capture probes complementary with chloramphenicol aptamer sequence portions are modified on the surface of a gold electrode and used for combining aptamer loci without being occupied by the chloramphenicol, the magnetic nanoparticles are marked, markers of the magnetic nanoparticles are converted into Prussian blue with electrochemical activity by the aid of an electrochemical method, and output signals inversely proportional to the concentration of the chloramphenicol are acquired according to current of electrochemical reduction peaks. According to the sensor, magnetic separation, magnetic enrichment and signal amplification are integrated, time and reagent cost are saved, detection steps and use processes of experimental instruments are simplified, the sensor is rapid, low in cost and high in operability, and sensitive detection of the chloramphenicol is achieved.

Description

technical field [0001] The invention relates to the technical field of biosensors for antibiotic detection, in particular to a magnetic separation-signal amplification integrated chloramphenicol detection biosensor and a method. Background technique [0002] Antibiotics can inhibit and kill bacteria and microorganisms, and are widely used in agriculture, animal husbandry, biological science and food engineering and other fields. Chloramphenicol is the world's first broad-spectrum antibiotic produced in large quantities by synthetic methods, with low cost and good antibacterial effect, and is currently one of the most widely used antibiotics. However, chloramphenicol can remain in a large amount in animals, which has repeatedly caused serious food safety incidents, posed a great threat to human health, and has attracted the attention of the society and the government. Therefore, it is very important to develop a reliable and simple method for the detection of chloramphenicol...

Claims

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Application Information

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IPC IPC(8): G01N27/416G01N27/327
CPCG01N27/3278G01N27/416
Inventor 李延斌傅迎春曹露露
Owner ZHEJIANG UNIV
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