Method for preparing multi-layer bio-based vesica capable of releasing insulin

A technology of insulin and bio-based macromolecules, applied in the field of functional materials, can solve the problems of poor biocompatibility and limited application, and achieve the effect of improving stability

Active Publication Date: 2015-11-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing research reports, in some studies, polymers with poor biocompatibility such as non-degrad

Method used

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  • Method for preparing multi-layer bio-based vesica capable of releasing insulin
  • Method for preparing multi-layer bio-based vesica capable of releasing insulin
  • Method for preparing multi-layer bio-based vesica capable of releasing insulin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation of embodiment 1, CS-g-CPBA

[0021] Take 1.790g CPBA (5mmol) and dissolve it in 200ml deionized water, add co-catalyst 1.725g N-hydroxysuccinimide (NHS, 15mmol) and 0.960g 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide Amine (EDC, 5mmol), activated under magnetic stirring for 0.5h. Then oligochitosan (5 mmol) in the amount of CPBA and the like was added to the solution, and after reacting for 72 hours at 25° C., the sample was dialyzed for one week and freeze-dried.

Embodiment 2

[0022] The preparation of embodiment 2, Alg-g-APBA

[0023] Dissolve Alg (6 mmol) in 150 ml of water, add co-catalysts 2.070 g NHS (18 mmol) and 1.152 g EDC (6 mmol), and activate for 0.5 h under magnetic stirring. Then APBA (4 mmol) was added to the solution, and after reacting at 25° C. for 72 h, the sample was dialyzed for 7 days and freeze-dried.

Embodiment 3

[0024] Embodiment 3, ((Alg-g-APBA / CS-g-CPBA) 5 ) SiO 2 preparation of

[0025] Prepare Alg-g-APBA, CS-g-CPBA, and insulin into a solution with a concentration of 2mg / ml at pH=5, SiO 2 -NH 2 Prepare a suspension with pH=5 and a concentration of 2 mg / ml, and disperse evenly after ultrasonic treatment for 30 minutes. Take 20ml prepared SiO 2 -NH 2 The suspension was first slowly added dropwise to 20ml of insulin solution, reacted for 30min, then slowly added dropwise with 20ml of Alg-g-APBA solution, reacted for 30min, centrifuged, washed with pH=5 aqueous solution for 3 times, and the nanoparticles were redispersed to pH= 5 solution (20ml) to complete the first layer of assembly. The first layer of assembly suspension was added dropwise to 20ml of CS-g-CPBA solution, centrifuged after 30min of reaction, washed 3 times with an aqueous solution of pH=5, and the nanoparticles were redispersed in an aqueous solution of pH=5 (20ml), Complete the second layer assembly. The ass...

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Abstract

The invention provides a method for preparing a multi-layer bio-based vesica capable of releasing insulin, and belongs to the field of functional materials. The method uses a bio-based macro-molecule as a main raw material, and comprises the following steps: respectively modifying sodium alginate (Alg) and chitosan oligosaccharide (CS) by function monomer 3-aminobenzeneboronic acid (APBA) and 3-carboxyphenylboronic acid (CPBA) to synthesize Alg-g-CPBA grafted copolymer and CS-g-CPBA grafted copolymer; preparing a multi-layer bio-based macromolecular microsphere by using insulin pre-embedded silicon dioxide nano-particles as a template by virtue of layer-by-layer self-assembly; adding CaCl2 to enable the Alg layer to perform physical crosslinking so as to improve the microsphere stability; and finally, removing the template to prepare the multi-layer bio-based vesica capable of releasing insulin. The prepared vesica has high stability, unique hollow structure and excellent biocompatibility, and has a covering amount on insulin being 60 percent; and controllable release of insulin can be realized under the stimulating action of glucose with different concentrations, and the release capacity is 80 percent. The vesica has a potential application value in the field of drug loading, controllable release and the like.

Description

technical field [0001] The invention discloses a method for preparing insulin-releasing multilayer bio-based vesicles, which belongs to the field of functional materials. Background technique [0002] Diabetes is one of the major diseases that endanger human health. Traditional treatment methods have brought great pain to patients. Intelligent stimulus-responsive drug delivery polymers have the functions of controllability and intelligent control of drug delivery, which can avoid traditional treatment. The shortcomings of the method provide a new method for the treatment of diabetes. Among these methods, phenylboronic acid and its derivatives have been widely studied for their stable system, controllable regulation of insulin delivery and continuous detection of blood glucose. Phenylboronic acid and its derivatives exist in two different forms: when pH < phenylboronic acid pKa value, it is the unionized hydrophobic form; when pH > phenylboronic acid pKa value, it is t...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K38/28A61K47/36A61P3/10C08B37/04C08B37/08C08J3/24C08J3/12
CPCY02P20/582
Inventor 施冬健冉茂双倪忠斌李小杰陈明清
Owner JIANGNAN UNIV
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