Method for isolating extracellular vesicles using cations

A technology for separating cells and cations, which is applied in the field of separating extracellular vesicles by using cations, which can solve the problems of difficult and efficient development of antibodies and protein ligands, low efficiency, and high cost
CN111148828APending Publication Date: 2020-05-12ROSETTA EXOSOME CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ROSETTA EXOSOME CO LTD
Publication Date
2020-05-12

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Abstract

The present invention relates to a method for isolating extracellular vesicles using cations, and more particularly, to a method for isolating extracellular vesicles from various samples by using theaffinity between the extracellular vesicles and cations. A method for isolating extracellular vesicles according to the present invention does not require expensive equipment, can be applied irrespective of sample amount, and has the advantage of being capable of efficiently isolating the extracellular vesicles while preserving the shape or characteristics thereof. Moreover, the method according to the present invention can be combined with existing isolation methods to maximize extracellular vesicle isolation efficiency, and can be applied to disease diagnosis, disease treatment, and multi-omics research using isolated extracellular vesicles, as well as to research on the properties of extracellular vesicles.
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Description

technical field

[0001] The invention relates to a method for separating extracellular vesicles by using cations, in particular to a method for separating extracellular vesicles from various samples by using the affinity of extracellular vesicles to various types of cations. Background technique

[0002] Extracellular vesicles are nano-sized vesicles that are naturally released by all living organisms or cells, from humans to bacteria, through pervasive cellular mechanisms. Extracellular vesicles derived from eukaryotic cells are involved in the differentiation of erythrocytes, the regulation of immune responses, etc., especially in the microenvironment of cancer cells, which play an important role in the development, metastasis or angiogenesis of cancer. Therefore, the application of extracellular vesicles as diagnostic labels in various diseases including cancer has received much attention.

[0003] Similar to extracellular vesicles of eukaryotic cells, extracellular vesic...

Claims

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