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Biological ink for 3D (Three Dimensional) printing and preparation method thereof

A bio-ink, 3D printing technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of unsatisfactory biological activity, chemical residue, unsatisfactory effect, etc., to achieve individual targeting and immunogenicity Low, safe effect

Active Publication Date: 2018-07-24
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] (1) When synthetic gels such as polydihydroxypropyl fumarate (PPF), poly D, L-lactide (PDLLA), polyε-caprolactone (PCL), polycarbonate, etc. are used as printing inks The molding speed is fast, but photosensitive resin (mainly composed of oligomers, active monomers, photoinitiators and other additives) needs to be added during the preparation, so the safety needs to be further carefully evaluated;
[0004] (2) Synthetic polymer materials including polymers listed in "(1)", in addition to polyurethane (PU), polyethylene (PE), polyacrylate (Acrylic), which have the advantages of safety and easy mass production, However, some materials have chemical residues, and when applied to 3D printing bio-inks, there are many problems such as poor degradability, unknown toxicity of degradation product fragments and unsatisfactory biological activity;
Therefore, the final effect of this method is often unsatisfactory

Method used

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  • Biological ink for 3D (Three Dimensional) printing and preparation method thereof
  • Biological ink for 3D (Three Dimensional) printing and preparation method thereof
  • Biological ink for 3D (Three Dimensional) printing and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A kind of 3D printing biological ink, comprises the raw material of following mass percentage and is made of:

[0035] Extracellular Vesicle Suspension 10%

[0036] Pig dermal soft tissue derived extracellular matrix nanofiber powder 5%

[0037] Hydrochloric acid 1%

[0038] Ultrapure water balance.

[0039] The 3D printing bio-ink is prepared according to the following method:

[0040] Mix pig dermis and soft tissue-derived extracellular matrix nanofiber micropowder, hydrochloric acid and ultrapure water, stir until completely dissolved, adjust the pH to 6.5 with sodium bicarbonate solution, add extracellular vesicle suspension, mix evenly, centrifuge, and bioink material.

[0041] 3D printing forming method, comprising the steps of:

[0042] (1) 3D bioprinting: use the 3D bioprinter to print the composite ink material from the ink injection cylinder at 15°C through the needle to the receiving platform at 8°C according to the established digital model, and store i...

Embodiment 2

[0045] A kind of 3D printing biological ink, comprises the raw material of following mass percentage and is made of:

[0046] Extracellular Vesicle Suspension 30%

[0047] Pig dermal soft tissue-derived extracellular matrix nanofiber powder 9%

[0048] Acetic acid 1%

[0049] Ultrapure water balance.

[0050] The 3D printing bio-ink is prepared according to the following method:

[0051] Mix pig dermis soft tissue-derived extracellular matrix nanofiber micropowder, acetic acid and ultrapure water, stir until completely dissolved, adjust the pH to 6.9 with sodium bicarbonate, add extracellular vesicle suspension, mix well, and centrifuge. bioink material.

[0052] 3D printing forming method, comprising the steps of:

[0053] 3D printing was carried out according to the 3D printing molding method of Example 1, and then washed with water and cross-linked to finalize the shape.

Embodiment 3

[0055] A kind of 3D printing biological ink, comprises the raw material of following mass percentage and is made of:

[0056] Extracellular vesicle suspension 20%;

[0057] Bovine dermis soft tissue-derived extracellular matrix nanofiber powder 15%;

[0058] Phosphoric acid 0.1%;

[0059] Ultrapure water balance.

[0060] The 3D printing bio-ink is prepared according to the following method:

[0061] Mix bovine soft tissue-derived extracellular matrix nanofiber powder, phosphoric acid and ultrapure water, stir until completely dissolved, adjust the pH to 6 with sodium phosphate, add extracellular vesicle suspension, mix well, and centrifuge to obtain bioink materials.

[0062] 3D printing forming method, comprising the steps of:

[0063] Carry out 3D printing according to the 3D printing molding method of Example 1, then wash with water, 0.5% (according to the amount of 3D printing bio-ink) glutaraldehyde cross-linking to set the shape.

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Abstract

The invention discloses biological ink for 3D (Three Dimensional) printing and a preparation method thereof. The biological ink for the 3D printing is prepared from the following raw materials in percentage by mass: 10 to 30 percent of an extracellular vesicle mixed suspension solution, 5 to 15 percent of a biological macromolecular material, 0.1 to 1 percent of dissolution promoter and the balance of water. The biological ink disclosed by the invention can be used for simulating extracellular matrix component, structure and biological activity characteristics better by utilizing advantages ofextracellular vesicles and characteristics of the biological macromolecular material and hydrogel.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to the field of 3D bioprinting technology, in particular to a 3D printing bioink and a preparation method thereof. Background technique [0002] The cases of 3D printing technology used in clinical medicine are no longer uncommon. The combination of this technology and medical treatment mainly includes the following types: ①preoperative diagnosis and surgical rehearsal model; ②implant preparation (bones, teeth, soft tissue organs, etc.) ; ③ External medical consumables (maxillofacial shaping braces, etc.). At present, the printing technology of hard tissue scaffolds such as bones and teeth is relatively mature, but the printing of soft tissue scaffolds still needs to be further improved. Most of the existing bio-inks available for soft tissue printing are polymer hydrogels, including synthetic polymer materials and natural hydrogels such as collagen: [0003] (1) When syntheti...

Claims

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Application Information

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IPC IPC(8): A61L27/36B33Y70/00
CPCA61L27/3604A61L27/3633A61L2430/34B33Y70/00
Inventor 陈蕾李志勇祁少海陈永明吴军傅炀
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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