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Method for layer-layer self-assembling and fixing biological active factor on tissue engineering material

A bioactive factor and tissue engineering technology, applied in medical science, prosthesis, etc., can solve the problems of unable to achieve stable fixation of bioactive factors, loss of biological activity of bioactive factors, etc., and achieve the effect of simple and feasible process and good repeatability.

Inactive Publication Date: 2005-12-28
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Simple physical adsorption cannot achieve the purpose of stably immobilizing bioactive factors, while chemical immobilization may cause bioactive factors to lose their biological activity.

Method used

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  • Method for layer-layer self-assembling and fixing biological active factor on tissue engineering material
  • Method for layer-layer self-assembling and fixing biological active factor on tissue engineering material
  • Method for layer-layer self-assembling and fixing biological active factor on tissue engineering material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: UV-Vis spectrum characterizes the process of quartz surface layer-layer self-assembly basic fibroblast growth factor (bFGF) / chondroitin sulfate (CS)

[0028] Soak the quartz glass grafted with KH-550 in 3% acetic acid solution for 15 minutes to protonate (ie cationize) the amino groups on the surface of the quartz glass, and then put it into 0.1M chondroitin sulfate containing 1mg / ml Soak in NaCl solution for 15 minutes. The quartz glass assembled with a layer of chondroitin sulfate was put into 0.1M NaCl solution and rinsed 3 times for 2 minutes each time. After rinsing, the quartz glass was soaked in a PBS solution (pH 7.4) of 10 μg / ml rhodamine-labeled bFGF (Rd-bFGF) for 15 minutes, and then rinsed 3 times with PBS (pH 7.4), each time Rinse for 2 minutes. By repeating the above steps, a quartz glass surface assembled with multilayer bFGF / CS can be obtained. After each double layer was assembled, the absorption of Rd-bFGF at 560 nm was detected by UV-...

Embodiment 2

[0029] Example 2: The contact angle measurement instrument characterizes the process of quartz surface layer-layer self-assembly basic fibroblast growth factor (bFGF) / chondroitin sulfate (CS)

[0030] Soak the quartz glass grafted with KH-550 in 3% acetic acid solution for 15 minutes to protonate (ie cationize) the amino groups on the surface of the quartz glass, and then put it into 0.1M chondroitin sulfate containing 1mg / ml Soak in NaCl solution for 15 minutes. The quartz glass assembled with a layer of chondroitin sulfate was put into 0.1M NaCl solution and rinsed 3 times for 2 minutes each time. After rinsing, the quartz glass was soaked in 10 μg / ml bFGF in PBS solution (pH value 7.4) for 15 minutes, and then rinsed with PBS solution (pH value 7.4) for 3 times, each time for 2 minutes. By repeating the above steps, a quartz glass surface assembled with multilayer bFGF / CS can be obtained. Each assembled layer was measured with a surface tensiometer to measure the change o...

Embodiment 3

[0031] Embodiment 3: UV-visible spectrum evaluates the stability of basic fibroblast growth factor (bFGF) / chondroitin sulfate (CS) multilayer film on quartz surface

[0032] Soak the quartz glass grafted with KH-550 in 3% acetic acid solution for 15 minutes to protonate (ie cationize) the amino groups on the surface of the quartz glass, and then put it into 0.1M chondroitin sulfate containing 1mg / ml NaCl solution for 15 minutes. The quartz glass assembled with a layer of chondroitin sulfate was put into 0.1M NaCl solution and rinsed 3 times for 2 minutes each time. After rinsing, the quartz glass was soaked in a PBS solution (pH 7.4) of 10 μg / ml rhodamine-labeled bFGF (Rd-bFGF) for 15 minutes, and then rinsed 3 times with PBS (pH 7.4), each time Rinse for 2 minutes. Repeat the above steps to obtain a quartz glass surface with 10 layers of Rd-bFGF / CS assembled. It was soaked in PBS buffer solution, and the ultraviolet-visible absorption of Rd-bFGF on the quartz glass surface...

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Abstract

The invention discloses a method for immobilizing bioactive factors on tissue engineering materials by adopting layer-layer self-assembly. This method utilizes the charging characteristics of natural bioactive factors in a certain pH environment, and through self-assembly with other polyelectrolyte layers with opposite charges, the bioactive factors are assembled on the surface of ionized materials to obtain biologically active tissues. Engineering Materials. The method of the invention does not destroy the biological activity of biological active factors, and does not affect the specific microstructure of tissue engineering materials, and is suitable for the modification of tissue engineering scaffolds with complex structures. The process of the invention is simple and feasible, and has good repeatability, and the constructed bioactive scaffold can be widely used in various tissue engineering fields such as skin, cartilage, bone, blood vessel, nerve, tendon, and heart valve.

Description

technical field [0001] The invention relates to bioactivation modification of tissue engineering materials, in particular to a method for immobilizing bioactive factors on tissue engineering materials by layer-layer self-assembly technology. Background technique [0002] Tissue engineering is an interdisciplinary research topic involving medicine, chemistry, biology, materials science and other disciplines and fields. In the study of tissue engineering, the effective fixation of bioactive factors to the surface of tissue engineering materials and the construction of bioactive scaffold materials are important links, which directly affect the proliferation and functional expression of target cells. Biologically active factors are a class of polypeptide substances that bind to specific, high-affinity receptors on cell membranes and ultimately stimulate cell proliferation and other biological effects. Their effects on target cells appear to be multifun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/54
Inventor 高长有马列周杰毛峥伟沈家骢
Owner ZHEJIANG UNIV
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