Collagen coated microcarrier for animal cell culture and preparation method thereof

A collagen and microcarrier technology, applied in animal cells, biochemical equipment and methods, tissue culture, etc., can solve the problems of weak protein connection, biocompatibility and unsatisfactory cell culture effect, and achieve optimal cell culture. effect, reduction in coating amount, cost reduction effect
CN103215217BActive Publication Date: 2015-08-19INST OF PROCESS ENG CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF PROCESS ENG CHINESE ACAD OF SCI
Publication Date
2015-08-19

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Abstract

The invention discloses a collagen-coated micro-carrier used in animal cell culture, and a preparation method thereof. According to the micro-carrier provided by the invention, konjac glucomannan microspheres are adopted as a substrate, and are coated by using collagen. The micro-carrier preparation method comprises the steps that: an activating reagent and a catalyst are added into the substrate microspheres, and an activation reaction is carried out in an alkaline environment; the prepared microspheres are cleaned and are vacuum-dried; the microspheres are added into a buffering solution, and collagen is added, such that a coupling reaction is carried out; the system after reaction is well mixed, and is added into water, such that collagen is coated and agglomerated on the surfaces of the microspheres; and the microspheres are washed; the microspheres are added into a reaction vessel; a crosslinking reagent and a PBS buffering solution are added, and the mixture is stirred, such that a reaction is carried out; after reaction, the product is washed, such that the micro-carrier product is obtained. The micro-carrier has uniform collagen coating on the surface, and the collagen is firmly connected, such that cell rapid adhesion and high-density growth can be facilitated. Also, the prepared micro-carrier has the advantages of low cost, short period, and suitability for industrialized productions.
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Description

technical field

[0001] The method relates to a microcarrier for animal cell culture and a preparation method thereof, in particular to a collagen-coated microcarrier for animal cell culture and a preparation method thereof, belonging to the field of biochemical engineering. Background technique

[0002] Microcarriers are a type of non-toxic, non-rigid, uniform density, and usually transparent small particles used in cell culture, which can enable anchorage-dependent cells to grow in a single layer on the particle surface during suspension culture, thereby increasing the number of cells. The attached growth area is conducive to the large-scale cultivation and collection of cells. Microcarrier culture is currently the main technology used for large-scale culture of animal cells. Large-scale cultivation of anchorage-dependent animal cells using microcarrier systems is the main production method adopted by biopharmaceutical companies at home and abroad, and is used for the prod...

Claims

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