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PLGA-PEG-PLGA inlaid mineralized collagen coating and its preparation method

A PLGA-PEG-PLGA, mineralized collagen technology, applied in chemical instruments and methods, layered products, metal layered products, etc., can solve the problem of undiscovered composite coatings, and achieve high-efficiency loading and slow release. , wide application prospects, the effect of improving sustained release behavior

Inactive Publication Date: 2012-04-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Relevant studies (Song ZM, et al. Journal of Colloid and Interface Science, 2011, 354:116-123) reported that the temperature-sensitive properties of PLGA-PEG-PLGA enable in-situ structure changes with temperature to achieve effective drug loading and release. The purpose of medicine, but it is generally used in the state of its block hydrogel, and there is no report of compounding it with other materials to make a coating

Method used

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  • PLGA-PEG-PLGA inlaid mineralized collagen coating and its preparation method
  • PLGA-PEG-PLGA inlaid mineralized collagen coating and its preparation method

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preparation example Construction

[0015] The preparation method of the mineralized collagen coating inlaid with the PLGA-PEG-PLGA copolymer involved in the present invention comprises the following steps:

[0016] 1. Surface pretreatment of the metal substrate: including cleaning the surface of the metal substrate, controlling the roughness, and treating the oxide layer, etc.

[0017] 2. Prepare the electrolyte, and use electrochemical deposition to obtain a porous mineralized collagen coating on the surface of the metal implant.

[0018] In the present invention, the prepared electrolyte contains Ca 2+ 、H 2 PO 4 - 、H 2 o 2 and collagen, their concentrations are 4~8mmol / L, 8~16 mmol / L, 15~20 mmol / L and 0.1~0.6g / L respectively, and the pH of the electrolyte is 4.3~4.6 (use 0.25~1mol / L adjusted by NaOH solution). The metal substrate is the cathode, the platinum electrode is the anode, the electrolyte is placed in a water bath at a temperature of 37 °C, the distance between the cathode and the anode is 1-2...

Embodiment 1

[0027] Dissolve collagen in 0.005mol / L acetic acid, stir to form a solution with a collagen concentration of 0.5g / L, add Ca(NO 3 ) 2 ·6H 2 O and NH 4 h 2 PO 4 Dissolve in deionized water, mix with collagen solution and a small amount of hydrogen peroxide to form calcium, phosphorus, H 2 o 2, and collagen concentrations of 8 mmol / L, 16 mmol / L, 15 mmol / L, and 0.6 g / L electrolytes, and the pH of the electrolyte was adjusted to 4.3 with NaOH solution. The titanium plate after surface pretreatment was used as the cathode, the platinum electrode was used as the anode, and the distance between the cathode and the anode was 1cm. Take 15ml of the electrolyte and place it in a water bath at 37°C, using a constant voltage deposition mode with a voltage of 2.5V. After 30 minutes of deposition, the titanium substrate was taken out, washed with deionized water, and then air-dried naturally to obtain a porous mineralized collagen gradient coating. PLGA-PEG-PLGA was added to deionized ...

Embodiment 2

[0029] Dissolve collagen in 0.005mol / L acetic acid, stir to form a solution with a collagen concentration of 0.5g / L, add Ca(NO 3 ) 2 ·6H 2 O and NaH 2 PO 4 Dissolve in deionized water, mix with collagen solution and a small amount of hydrogen peroxide to form calcium, phosphorus, H 2 o 2 , collagen concentrations of 4 mmol / L, 8 mmol / L, 20 mmol / L, and 0.1 g / L electrolytes, and adjust the pH of the electrolyte to 4.6 with NaOH solution. The titanium plate after surface pretreatment was used as the cathode, and the platinum electrode was used as the anode. The distance between the cathode and the anode was 2cm. Take 15ml of electrolyte and place it in a water bath at 37°C. The constant voltage deposition mode was used with a voltage of 2.5V. After 45 minutes of deposition, the titanium substrate was taken out, washed with deionized water, and then air-dried naturally to obtain a porous mineralized collagen gradient coating. PLGA-PEG-PLGA was added to deionized water to prep...

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Abstract

The invention discloses a PLGA (poly(lactic-co-glycolic acid))-PEG (poly(ethylene glycol))-PLGA inlaid mineralized collagen coating and its preparation method. The method of the invention conducts electrochemical deposition, adjusts deposition parameters, constructs a mineralized collagen coating with a porous structure on a metal implant surface, and inlays a temperature sensitive polymer PLGA-PEG-PLGA into the structure, so that the coating becomes an excellent drug slow release system and can be used for controlled release of biotic factors, antibiotics and other drugs in vivo. PLGA-PEG-PLGA can make sol-gel conversion with temperature variation. On account of the characteristic, biotic factors and antibiotics, etc. are loaded under a sol state of PLGA-PEG-PLGA, under the gel state of which the drugs can be released, thus optimizing the slow release behavior of the coating. The coating prepared by the method of the invention can make use of mineralized collagen to transmit biological signals and the temperature sensitive polymer to control drug release effectively, so that the coating can has high biological responsiveness as well as a good drug slow release function simultaneously, thus boasting wide application prospects in hard bone tissue repair field.

Description

technical field [0001] The invention relates to a metal implant surface PLGA-PEG-PLGA (the English full name of PLGA is poly(lactic-co-glycolic acid), and the Chinese standard name is polylactic acid - glycolic acid copolymer; PEG's English full name is poly (ethylene glycol), and the Chinese standard name is polyethylene glycol) copolymer-embedded mineralized collagen coating and its preparation method. Background technique [0002] Mineralized collagen is a complex of calcium phosphate salts and collagen, similar in structure and composition to natural bone. Studies have shown (Dawson JI, et al. Biomaterials, 2009, 29(21): 3105-3116) that the composite material has excellent biomimetic properties, which can effectively promote the process of osseointegration and improve the ability of new bone formation. Collagen molecules and phosphate can be assembled on the metal substrate by means of electrochemical deposition, spin coating, and biomimetic methods to form a mineralize...

Claims

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Application Information

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IPC IPC(8): B32B9/04B32B15/00C25D9/02
Inventor 翁文剑凌婷程逵杜丕一韩高荣
Owner ZHEJIANG UNIV
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