Preparation method of collagen coating embedded with chitosan microspheres on medical metal implant surface

A technology of chitosan and implants, which is applied in the field of preparation of chitosan microsphere collagen coating embedded on the surface of medical metal implants, to achieve the effect of improving slow-release behavior, wide application prospects, high-efficiency loading and slow release

Inactive Publication Date: 2013-05-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although there have been studies on embedding chitosan molecules into the coating for sustained release of drugs, there has been no report on the study of embedding chitosan microspheres in mineralized collagen coatings for sustained release of drugs / biomolecules

Method used

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  • Preparation method of collagen coating embedded with chitosan microspheres on medical metal implant surface
  • Preparation method of collagen coating embedded with chitosan microspheres on medical metal implant surface
  • Preparation method of collagen coating embedded with chitosan microspheres on medical metal implant surface

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Chitosan is dissolved in the acetic acid solution of 166.7mM, prepares the chitosan acetic acid solution of 12.5g / L, adjusts the pH value of chitosan acetic acid solution to be 5.0;

[0026] Dissolve sodium tripolyphosphate (TPP) in deionized water to prepare a 12.5 g / L sodium tripolyphosphate solution. Under the stirring of a magnetic stirrer, use a syringe to drop the sodium tripolyphosphate solution into the chitosan acetic acid solution drop by drop, the volume ratio of the chitosan acetic acid solution to the sodium tripolyphosphate solution is 3:2, keep stirring for 1h, Obtain suspension. Centrifuge the suspension liquid at high speed (3000r / min) for 15min in a high-speed centrifuge, filter the upper layer liquid, collect the lower layer sediment, and dry it in an oven at 37°C. The prepared chitosan microspheres have the following appearance: figure 1 shown.

[0027] Preparation of electrolyte: Ca(NO 3 ) 2 ·6H 2 O was dissolved in water and prepared as Ca(NO ...

Embodiment 2

[0031] Chitosan was dissolved in 166.7mM acetic acid solution to prepare 12.5g / L chitosan acetic acid solution. Regulate the pH value of chitosan acetic acid solution to be 5.0;

[0032] Dissolve sodium tripolyphosphate (TPP) in deionized water to prepare a 12.5 g / L sodium tripolyphosphate solution. Under stirring with a magnetic stirrer, use a syringe to drop the sodium tripolyphosphate solution into the chitosan acetic acid solution drop by drop, the volume ratio of the chitosan acetic acid solution to the sodium tripolyphosphate solution is 3:2, keep stirring for 1h, Obtain suspension. Centrifuge the suspension at a high speed (3000r / min) for 15 minutes in a high-speed centrifuge, filter off the upper layer, collect the lower layer of sediment, and dry it in an oven at 37°C to obtain chitosan microspheres;

[0033] Preparation of electrolyte: CaCl 2 Dissolved in water, formulated as CaCl with a calcium concentration of 80mM 2 aqueous solution, the NaH 2 PO 4 Dissolved...

Embodiment 3

[0037] Chitosan was dissolved in 166.7mM acetic acid solution to prepare 12.5g / L chitosan acetic acid solution. The pH value of regulating chitosan acetic acid solution is 6.0;

[0038] Dissolve sodium tripolyphosphate (TPP) in deionized water to prepare a 12.5 g / L sodium tripolyphosphate solution. Under stirring with a magnetic stirrer, use a syringe to drop the sodium tripolyphosphate solution into the chitosan acetic acid solution drop by drop, the volume ratio of the chitosan acetic acid solution to the sodium tripolyphosphate solution is 3:2, keep stirring for 1h, Obtain suspension. Centrifuge the suspension at a high speed (3000r / min) for 15 minutes in a high-speed centrifuge, filter off the upper layer, collect the lower layer of sediment, and dry it in an oven at 37°C to obtain chitosan microspheres;

[0039] Preparation of electrolyte: Ca(NO 3 ) 2 ·6H 2 O was dissolved in water and prepared as Ca(NO 3 ) 2 aqueous solution, the NH 4 h 2 PO 4 Dissolved in wate...

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Abstract

The invention discloses a preparation method of a collagen coating embedded with chitosan microspheres on a medical metal implant surface. The mineralized collagen coating embedded with the chitosan microspheres is formed on a substrate by adopting an electrochemical deposition method and adjusting parameters such as a pH (potential of hydrogen) value and on-load voltage of an electrolytic solution. The method is simple in technology; the prepared coating is embedded with the chitosan microspheres; and as the chitosan microspheres has an effect of effective controlled release of biological factors or drugs such as antibiotics, the coating has high biological responsiveness, a good drug sustained release function and a wide application prospect in the field of hard-bone tissue repair.

Description

technical field [0001] The invention relates to a preparation method of a collagen coating embedded with chitosan microspheres (Chitosan nanospheres) on the surface of a medical metal implant. Background technique [0002] Based on the concept of bionics, using collagen as a template, the mineralization is modulated in the calcium-phosphorus salt solution to obtain a composite coating with a hierarchical structure imitating natural bone, which is composed of calcium phosphate mineral phase and collagen self-assembly and mineralization. The reactivity of the collagen has received extensive attention (Michael Maas, et al. Nano Lett.2011, 11, 1383–1388). At present, the methods for preparing mineralized collagen coatings on metal substrates mainly include electrochemical deposition, spin coating, and Biomimetic deposition method. These methods all yield a uniformly distributed coating of mineralized collagen. Among them, electrochemical deposition has attracted the attention ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C25D9/08C25D15/00
Inventor 翁文剑孔子强程逵王慧明林军
Owner ZHEJIANG UNIV
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