The invention provides a programmable control micro-fluidic
chip based on a liquid-liquid
electrowetting effect. The programmable control micro-fluidic
chip comprises a lower substrate, a drive
electrode array, a lower substrate hydrophobic insulating layer, at least one
liquid drop, an upper substrate hydrophobic layer, a conductive layer and an upper substrate from bottom to top, wherein cofferdams are added to the peripheries of the upper substrate and the lower substrate to form a
closed cavity. The hydrophobic insulating layer is formed by a porous
polymer film and a liquid capable of improving electric wettability and has the advantages of being good in electric
wetting reversibility, reusable and resistant to
voltage breakdown, high temperature and chemical reagents, the effect of the hydrophobic insulating layer is not affected after long-time storage, so that the service life of a device is prolonged, the efficiency of the device is improved, and the application range of the device is broadened; besides, the pressing mode of the drive
electrode array can be programmed, therefore, operation processes such as rapid movement, combination, reaction and the like of different liquid drops are executed in different positions according to preset parameters.