Preparation method for medical metal implant surface chitosan quaternary ammonium salt/collagen composite coating

A chitosan quaternary ammonium salt and composite coating technology, which can be used in coatings, prostheses, medical science, etc., can solve the problems of limited coating control, limited improvement of bearing capacity, and decreased electric field responsiveness, and achieve release behavior. Controllable adjustment, increased capacity, the effect of wide application prospects

Active Publication Date: 2015-10-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to the deposition mechanism of mineralized collagen, a pH gradient will be generated near the substrate during the electrochemical deposition process, and chitosan will lose protons at a certain depth deposited to the coating as the pH increases, and the chitosan that loses protons responds to the electric field However, the property of chitosan decreased sharply, and the enrichmen

Method used

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  • Preparation method for medical metal implant surface chitosan quaternary ammonium salt/collagen composite coating
  • Preparation method for medical metal implant surface chitosan quaternary ammonium salt/collagen composite coating
  • Preparation method for medical metal implant surface chitosan quaternary ammonium salt/collagen composite coating

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Embodiment 1

[0023] 1) quaternary ammonium root substitution degree is 98%, molecular mass is 30000 2-hydroxypropyltrimethylammonium chloride chitosan is dissolved in distilled water, preparation concentration is the chitosan quaternary ammonium salt aqueous solution of 0.5g / L, put Stand-by at 4°C; dissolve Beiling liquid type I collagen in 0.005mol / L acetic acid to prepare a collagen acetic acid solution with a concentration of 0.5g / L, and place it at 4°C for use;

[0024] 2) Preparation of electrolyte: Ca(NO 3 ) 2 ·6H 2 O is dissolved in water and prepared as Ca(NO 3 ) 2 aqueous solution, the NH 4 h 2 PO 4 Dissolved in water, prepared as NH with a phosphorus concentration of 80mmol / L 4 h 2 PO 4 aqueous solution, the Ca(NO 3 ) 2 Aqueous solution and NH 4 h 2 PO 4 The aqueous solution was stirred and mixed to obtain A solution. Mix Beiling liquid type I collagen, 2-hydroxypropyltrimethylammonium chloride chitosan solution with solution A to make the quaternary ammonium root ...

Embodiment 2

[0028] 1) quaternary ammonium root substitution degree is 98%, molecular mass is 30000 2-hydroxypropyltrimethylammonium chloride chitosan is dissolved in distilled water, preparation concentration is the chitosan quaternary ammonium salt aqueous solution of 0.5g / L, put Stand-by at 4°C; dissolve Beiling liquid type I collagen in 0.005mol / L acetic acid to prepare a collagen acetic acid solution with a concentration of 0.5g / L, and place it at 4°C for use;

[0029] 2) Preparation of electrolyte: Ca(NO 3 ) 2 ·6H 2 O is dissolved in water and prepared as Ca(NO 3 ) 2 aqueous solution, the NH 4 h 2 PO4 Dissolved in water, prepared as NH with a phosphorus concentration of 80mmol / L 4 h 2 PO 4 aqueous solution, the Ca(NO 3 ) 2 Aqueous solution and NH 4 h 2 PO 4 The aqueous solution was stirred and mixed to obtain A solution. Mix Beiling liquid type I collagen, 2-hydroxypropyltrimethylammonium chloride chitosan solution with solution A to make the quaternary ammonium root an...

Embodiment 3

[0033] 1) quaternary ammonium root substitution degree is 98%, molecular mass is 30000 2-hydroxypropyltrimethylammonium chloride chitosan is dissolved in distilled water, preparation concentration is the chitosan quaternary ammonium salt aqueous solution of 0.5g / L, put Stand-by at 4°C; dissolve Beiling liquid type I collagen in 0.005mol / L acetic acid to prepare a collagen acetic acid solution with a concentration of 0.5g / L, and place it at 4°C for use;

[0034] 2) Preparation of electrolyte: Ca(NO 3 ) 2 ·6H 2 O is dissolved in water and prepared as Ca(NO 3 ) 2 aqueous solution, the NH 4 h 2 PO 4 Dissolved in water, prepared as NH with a phosphorus concentration of 80mmol / L 4 h 2 PO 4 aqueous solution, the Ca(NO 3 ) 2 Aqueous solution and NH 4 h 2 PO 4 The aqueous solution was stirred and mixed to obtain A solution. Mix Beiling liquid type I collagen, 2-hydroxypropyltrimethylammonium chloride chitosan solution with solution A to make the quaternary ammonium root ...

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Abstract

The invention discloses a preparation method for a medical metal implant surface chitosan quaternary ammonium salt/collagen composite coating. The method includes the steps that an electrolyte containing calcium, phosphorus, collagen and chitosan quaternary ammonium salt is prepared; a platinum sheet serves as a counter electrode; a metal substrate washed through acid serves as a work electrode; and electrochemical deposition is performed in a multi-potential-step manner. The positive and negative condition and the amplitude of each step work electrode potential and deposition time of each segment are adjusted, so that deposition does not need to be performed multiple times, the purpose of controllable space distribution of the chitosan quaternary ammonium salt in a mineralized collagen coating on the surface of the substrate can be quickly and easily achieved, and in other words, the inner layer and the outer layer of the collagen coating are rich in chitosan quaternary ammonium salt. The feature that the chitosan quaternary ammonium salt is provided with positive charges and biological growth factors are carried in different space positions of the collagen coating, so that the biological growth factor release behavior can be controlled and adjusted. Meanwhile, the capacity of the coating for carrying biological growth factors is improved and the method has the wide application prospect in hard-bone tissue regeneration field.

Description

technical field [0001] The invention relates to a method for preparing a surface coating of a medical metal implant, in particular to a method for preparing a mineralized collagen coating on the surface of a medical metal implant. Background technique [0002] In the process of bone repair, the body's own cells produce different growth factors at different stages to regulate the smooth progress of osseointegration, and the release behavior of growth factors often determines the success of bone repair. Therefore, for new metal implant materials, it should not only have good biocompatibility, but also have good growth factor or drug loading capacity and controllable release behavior of the growth factors it carries. Usually, a layer of organic substances with slow-release function is coated on the surface of metal implants and growth factor agents are embedded to meet the requirements of biocompatibility, growth and bearing capacity, and release behavior control. However, coa...

Claims

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Application Information

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IPC IPC(8): A61L27/34A61L27/54C25D9/02C25D5/00
Inventor 翁文剑陈潞程逵王慧明林军
Owner ZHEJIANG UNIV
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