Method for constructing anti-blood coagulation blood vessel scaffold by utilizing endogenous NO donor
A vascular stent and anticoagulant technology, which is applied in the field of constructing anticoagulant vascular stent materials by using endogenous NO donors, can solve the problems of affecting the persistence of NO release, fast release rate, and limited application, and achieve inhibition of smooth muscle cells. Proliferation, prevention of vascular restenosis, wide selection of effects
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Embodiment 1
[0017] Embodiment 1: Utilize endogenous NO donor to construct the method for anticoagulant artificial vascular stent material, comprise the following steps in turn:
[0018] ① Preparation of polyethylenimine (SePEI) with organic selenium immobilized:
[0019] Dipropionyl diselenide (SeDPA) was first activated with EDC and NHS. Then mix the mixture with PEI(M w 25kD) in MES buffer (pH 6.0) for 2 hours. The product SePEI was separated by centrifugation. The Se content in SePEI polymer was quantified by ICP-MS.
[0020] ②L-b-L self-assembly method forms a multilayer bimolecular film on the surface of a porous vascular stent material formed by electrospinning polycaprolactone (PCL):
[0021] First, SePEI and polyglutamic acid (PGA) were formulated into 1 mg / ml solutions in PBS (pH 7.4), and polydiallyldimethylammonium chloride (PDDA) was dissolved in biological buffer CHES (2 -Cyclohexylaminoethanesulfonic acid) (pH 9.3) to make a 1 mg / ml solution. The surface of the PCL por...
Embodiment 2
[0022] Embodiment 2 The method for constructing an anticoagulant metal vascular stent material by using an endogenous NO donor comprises the following steps in sequence:
[0023] ①Preparation of polylysine (SePLL) loaded with organic selenium:
[0024] Dipropionyl diselenide (SeDPA) was first activated with EDC and NHS. Then mix the mixture with polylysine (PLL) (M w 25kD) in MES buffer (pH 6.0) for 2 hours. The product SePLL was isolated by centrifugation. The Se content in the SePLL polymer was quantified by ICP-MS.
[0025] ②L-b-L self-assembly method forms a multi-layer bimolecular film on the surface of the metal vascular stent material:
[0026] SePLL and polyalginic acid (ALG, M w 30kD) were prepared in PBS (pH 7.4) to form a 1mg / ml solution, and polydiallyldimethylammonium chloride (PDDA) was dissolved in CHES (pH 9.3) to form a 1mg / m1 solution. The surface of the finished metal vascular stent material is first coated with PDDA and ALG for 10 minutes each to fo...
Embodiment 3
[0027] Example 3 Method for Constructing Anticoagulant Co-spun Fiber Porous Artificial Vascular Stent Material Using Endogenous NO Donor
[0028] Co-spun fiber (PHA MCL / PLA) The inner surface of the porous artificial vascular stent material will be organic selenized polycation polymer——water-soluble chitosan (SeCS, M w 30kD) and polyanionic high polymer - polymalic acid (PMA, M w 10kD) is alternately coated by a layer-by-layer self-assembly method to form a multilayer bimolecular film of water-soluble chitosan / polymalic acid (SeCS / PMA); the number of L-b-L layers is 12 layers.
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