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Silk artificial blood vessel and preparation method thereof

An artificial blood vessel and blood vessel technology, applied in the field of biomedical engineering, can solve the problems of inability to meet the requirement of having a complete endothelial layer, slow degradation, etc., and achieve the effects of improving anticoagulant effect, reducing immunogenicity, and improving rigidity

Active Publication Date: 2015-07-22
CHONGQING ACAD OF ANIMAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are two problems in the preparation of artificial blood vessels using silk: (1) silk is composed of silk fibroin and sericin, which can be slowly degraded in vitro and in vivo
At present, none of the existing artificial blood vessels can meet the requirement of having a complete endothelial layer

Method used

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  • Silk artificial blood vessel and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043] The preparation of embodiment 1 artificial blood vessel

[0044] The preparation method of artificial blood vessel comprises the following steps:

[0045] (1) Preparation of vascular body

[0046] Weaving a tube with an inner diameter of 2-20mm and a length of 50-500mm with boiled silk to obtain the blood vessel body;

[0047] (2) Glycosylation of Sericin

[0048] Take a certain amount of sericin, dissolve it in triple distilled water to make the concentration 1-10g / 100mL, adjust the pH to 11 with dilute alkali, heat to about 30-70°C, stir for about 30-120min, and cool at room temperature. Then add dextran or mannan, adjust the pH to 10-12 with dilute alkali, stir for about 30-120 minutes, and freeze-dry to obtain a mixture freeze-dried powder of sericin and polysaccharide. Place the freeze-dried powder of the mixture in a desiccator, and react for 12-120 hours at 45-70°C and a relative humidity of 50-80% to obtain glycosylated sericin, and the obtained glycosylated ...

Embodiment 2

[0055] Application of embodiment 2 artificial blood vessels

[0056] (1) The preparation of artificial blood vessels refers to the method in Example 1. The selected artery for endothelial pre-lining is a rat artery; an artificial blood vessel with an inner diameter of 2 mm and a length of 20 mm is transplanted into the abdominal aorta of a rat, and the , the long-term patency rate is 100%;

[0057] (2) The preparation of artificial blood vessels is referring to the method of embodiment 1, and the selected artery of endothelial lining is porcine femoral artery; The artificial blood vessel with inner diameter is 5mm, long 120mm is transplanted in porcine femoral aorta, in the time of 1 year, long-term The patency rate was 86%;

[0058] (3) The preparation of the artificial blood vessel is referring to the method in Example 1. The selected artery of the endothelial pre-line is the porcine brachial artery; the artificial blood vessel with an internal diameter of 4 mm and a length...

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Abstract

Belonging to the field of biomedical engineering, the invention specifically related to a silk artificial blood vessel and a preparation method thereof. The artificial blood vessel includes a silk woven blood vessel body and glycosylated cross-linking sericin filling blood vessel body fiber gaps. The preparation method includes the steps of: preparation of the blood vessel body, compounding of the blood vessel body and glycosylated sericin, blood vessel surface modification and endothelial pre-coating. In the invention, glycosylated sericin is compounded with silk, then aldehyde is employed to crosslink sericin so as to reduce water solubility, the gaps between artificial blood vessel silk fiber are filled to prevent blood leakage, and the rigidity of the blood vessel is improved. The glycosylated sericin is located on the artificial blood vessel surface, can reduce immunogenicity and improve the anticoagulation effect, and makes the artificial blood vessel difficult to degrade, thus overcoming the two defects of easy degradation and strong immunogenicity in the prior art. And the silk artificial blood vessel has the advantages of simplicity and practicability, good quality and low price.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and in particular relates to a silk artificial blood vessel and a preparation method thereof. Background technique [0002] A large number of vascular grafts are required in surgical procedures such as coronary artery bypass grafting, vascular replacement, and endovascular fistula. Autologous blood vessels are the best choice. In the transplantation of autologous blood vessels, certain damages will be caused to the donor area, so people use allogeneic blood vessels such as human umbilical vein with mesh and xenografts such as calf internal jugular vein and thoracic main artery. Arteries and the like serve as vascular grafts. The source of allogeneic blood vessels is limited and has certain immunogenicity, so it must be treated with glutaraldehyde to weaken the antigenicity. Although the source of xenograft blood vessels is abundant, they also have certain immunogenicity and must be treated...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/50A61L33/12
Inventor 周婵曾秀黄勇马群忠王介平吕金凤王海燕刘彬
Owner CHONGQING ACAD OF ANIMAL SCI
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