Anticoagulation coating material and preparation method thereof
A coating material and anti-coagulation technology, applied in the field of anti-coagulation coating material and its preparation, can solve the problems of not lasting, unstable anti-coagulation effect, poor biocompatibility, etc. Anticoagulant effect, structural stabilization effect
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[0036] The method for preparing an anticoagulant coating material provided by the present invention includes the following steps:
[0037] (1) Prepare 2-(N-morpholine) ethanesulfonic acid (MES) buffer solution with a concentration of 0.1 mol / L and adjust the pH value to 6.4.
[0038] (2) Prepare sodium hyaluronate solution:
[0039] Take sodium hyaluronate (HA, molecular weight about 2 million) in 2-(N-morpholine) ethanesulfonic acid buffer solution (MES, 0.1M, pH=6.4) to fully dissolve it to obtain sodium hyaluronate solution. The concentration of sodium hyaluronate solution is 1mg / ml to 20mg / ml. Magnetic stirring can be used to accelerate the dissolution of sodium hyaluronate, stirring for 12 hours. The prepared sodium hyaluronate solution can be sealed and frozen for later use.
[0040] (3) Prepare hirudin solution:
[0041] Take the hirudin in the MES buffer, so that the hirudin is evenly dispersed in the MES buffer. To increase the solubility of hirudin, a surfactant can be add...
Example Embodiment
[0049] Example 1
[0050] An anticoagulant coating material includes sodium hyaluronate and hirudin. The sodium hyaluronate forms a gel network, and the hirudin is covalently bound to the sodium hyaluronate through an amide bond. The mass ratio of the hirudin to the sodium hyaluronate is 1:1. The anticoagulant coating material also includes a crosslinking agent, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) .
[0051] The preparation method of the anticoagulant coating material includes the following steps:
[0052] (1) Prepare 2-(N-morpholine) ethanesulfonic acid (MES) buffer solution with a concentration of 0.1 mol / L and adjust the pH value to 6.4.
[0053] (2) Prepare sodium hyaluronate solution.
[0054] Take sodium hyaluronate (HA, molecular weight about 2 million) in 2-(N-morpholine) ethanesulfonic acid buffer solution (MES, 0.1M, pH=6.4) to fully dissolve it to obtain sodium hyaluronate solution. The concentration of sodium hyaluronate so...
Example Embodiment
[0065] Example 2
[0066] An anticoagulant coating material includes sodium hyaluronate and hirudin. The sodium hyaluronate forms a gel network, and the hirudin is covalently bound to the sodium hyaluronate through an amide bond. The mass ratio of the hirudin to the sodium hyaluronate is 1:3. The anticoagulant coating material also includes a crosslinking agent, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) .
[0067] The preparation method of the anticoagulant coating material includes the following steps:
[0068] (1) Prepare 2-(N-morpholine) ethanesulfonic acid (MES) buffer solution with a concentration of 0.1 mol / L and adjust the pH value to 6.4.
[0069] (2) Prepare sodium hyaluronate solution.
[0070] Take sodium hyaluronate (HA, molecular weight about 2 million) in 2-(N-morpholine) ethanesulfonic acid buffer solution (MES, 0.1M, pH=6.4) to fully dissolve it to obtain sodium hyaluronate solution. The concentration of sodium hyaluronate so...
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