Method for perparing anticoagulant composed of chitosan-arginine

A technology of arginine and chitosan, which is applied in the field of preparation of blood-compatible materials, can solve the problems of unsatisfactory anti-coagulation properties of anti-coagulation materials, limited application of cytotoxicity, etc., and achieves improved anti-coagulation performance, The effect of reducing cytotoxicity and simple preparation process

Inactive Publication Date: 2004-08-11
TIANJIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the anticoagulant properties of anticoagulant materials developed at present are not very satisfactory, and chitosan and its derivatives are often crosslinked with glutaraldehyde to make membrane materials.
However, the cytotoxicity of glutaraldehyde limits its application in the biomedical field to some extent.

Method used

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  • Method for perparing anticoagulant composed of chitosan-arginine
  • Method for perparing anticoagulant composed of chitosan-arginine
  • Method for perparing anticoagulant composed of chitosan-arginine

Examples

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Embodiment 1

[0017] Weigh 13 grams of sodium periodate, dissolve it in 100 grams of deionized water, add 10 grams of glucose, place in a dark box, add 10 μl of hydrochloric acid dropwise to adjust the pH to acidity, react at room temperature for 12 hours, add barium acetate and sodium sulfate in turn, React with unreacted excess sodium periodate and barium ions respectively, centrifuge to remove the precipitate, and freeze-dry the supernatant to obtain oxidized glucuronide.

[0018] Get 2.0 grams of chitosan with a degree of deacetylation of 80% and a molecular weight of 5000, add it to the TEMED solution that is adjusted to a pH of 4.8 with HCl, add 0.0906 grams of 1-ethyl-3-(3-dimethylaminopropyl) Carbodiimide and 0.0546 g of N-hydroxy-succinimide were stirred evenly, then 0.082 g of arginine was added, reacted at room temperature for 8 hours, dialyzed in deionized water for 5 days, and dried at room temperature to obtain the final product. Dissolve 200 mg of refined chitosan-arginine co...

Embodiment 2

[0024] Get 2.0 grams of chitosan with a degree of deacetylation of 80% and a molecular weight of 5000, add it to the TEMED solution that is adjusted to a pH of 4.8 with HCl, add 0.3624 grams of 1-ethyl-3-(3-dimethylaminopropyl) Carbodiimide and 0.2184 g of N-hydroxy-succinimide were stirred evenly, then 0.328 g of arginine was added, reacted at room temperature for 8 hours, dialyzed in deionized water for 5 days, and dried at room temperature to obtain the final product. Dissolve 200 mg of refined chitosan-arginine conjugate in deionized water, add 10 mg of glucosin oxide, stir evenly, pour it into a plastic petri dish with a smooth surface, and react in a vacuum oven at 37 °C for 24 hours, and finally A crosslinked film was obtained. Cut the film sample into 5mm×10mm rectangular pieces, soak it in 0.9% normal saline for 24 hours, and use it as a sample for anticoagulant performance testing. Deacetylation degree 80%, and molecular weight 50,000, deacetylation degree 80% chito...

Embodiment 3

[0029] Get 2.0 grams of chitosan with a deacetylation degree of 80% and a molecular weight of 50,000, join in the TEMED / HCl buffer solution of pH 4.8, add 0.5436 grams of 1-ethyl-3-(3-dimethylaminopropyl) carbon Diimine and 0.3276 g of N-hydroxy-succinimide were stirred evenly, then 0.492 g of arginine was added, reacted at room temperature for 8 hours, neutralized, dialyzed in deionized water for 5 days, and freeze-dried to obtain the final product. Dissolve 200 mg of refined chitosan-arginine conjugate in deionized water, add 10 mg of glucosin oxide, stir evenly, pour it into a plastic petri dish with a smooth surface, and react in a vacuum oven at 37 °C for 24 hours, and finally A crosslinked film was obtained. The film sample was cut into 5 mm×10 mm rectangular pieces, soaked in 0.9% saline for 24 h, and used as a sample for anticoagulant performance testing; the obtained sample was designated as CS1-ArgC3-1. According to the same preparation steps and conditions, samples...

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Abstract

A Chitosan-arginine conjugate as anticoagulant material is prepared through dissolving chitosan in the solution of N, N, N'-tetramethyl ethanediamine, adding 1-ethyl-3-(3-dimethylamino propyl) carbodiimine, N-hydroxy-butanediimide and arginine, reacting while magnetic stirring, dialyzing, drying to obtain said conjugate, adding glucosyl aldehyde oxide, and cross-linking reaction to become film. Its advantages are high effect and low poison to cell.

Description

technical field [0001] The invention relates to a preparation method of a chitosan-arginine conjugate anticoagulant material, which belongs to the preparation technology of blood compatibility materials in the field of biomedical engineering. Background technique [0002] The main material basis of artificial organs is polymer biomaterials. Polymer biomaterials are often used for implantation in vivo, so as a polymer biomaterial, its biocompatibility must be considered first. Biocompatibility includes hemocompatibility and cell compatibility. For hemocompatibility, the most prominent problem is the coagulation of blood after the material comes into contact with blood. When many synthetic polymer materials are in contact with blood, a series of complex interactions will occur at the interface between the two, leading to blood coagulation reaction and thrombus formation, which limits its application in the medical field. Moreover, long-term contact b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/34A61L31/10A61L33/08
Inventor 刘文广张建荣姚康德
Owner TIANJIN UNIV
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