Biological material with functions of catalytic releasing NO and capturing EPCs and preparation method thereof

A biomaterial and functional technology, applied in the field of medical devices, can solve the problems of inflammation, difficulty in clinical treatment of stents, coagulation, etc., and achieve the effect of mild reaction conditions

Active Publication Date: 2019-05-10
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The applicant found that in the prior art, problems such as inflammation, blood coagulation, and hyperplasia often occur at the implantation site of the stent when the above-mentioned stent is implanted.

Method used

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  • Biological material with functions of catalytic releasing NO and capturing EPCs and preparation method thereof
  • Biological material with functions of catalytic releasing NO and capturing EPCs and preparation method thereof
  • Biological material with functions of catalytic releasing NO and capturing EPCs and preparation method thereof

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preparation example Construction

[0023] The embodiment of the present invention provides a method for preparing a biological material with NO catalytic release and EPCs capture function, which includes the following steps:

[0024] S1. Deposition of metal oxides

[0025] A metal oxide film is deposited on the surface of the material to be modified, and the deposition of the metal oxide film is conducive to increasing the strength and biocompatibility of the material, and is also conducive to the fixation of the azidated ortho-phenolic compound on the surface of the material.

[0026] The materials to be modified can be cardiovascular stents, artificial blood vessels, osteoinductive materials, etc. The introduction of functional biomolecules on the surface of such materials improves their anticoagulant properties, making them capable of inhibiting smooth muscle hyperplasia and promoting in situ re-endothelialization. Very broad application space.

[0027] Metal oxides are metal oxides with excellent biocompat...

Embodiment 1

[0050] The present embodiment provides a method for preparing a biological material with NO catalytic release and EPCs capture functions, which includes the following steps:

[0051] A layer of TiO thin film was deposited on the surface of the cobalt-nickel bare stent by magnetron sputtering. The deposition power was 600 W, the deposition time was 8 mins, and the film thickness was about 50 nm.

[0052] with N 2 After deoxygenation for 30 mins, PBS buffer with a pH of about 5 was used as a solvent, and the concentration was 0.01 mg / mL Ac-(DOPA)-Gly-(DOPA)-Lys(PEG5-Azido)-(DOPA)-Gly-(DOPA ) (abbreviated as ((DOPA) 4 -N 3 ), the same below), the scaffold was immersed in the solution and reacted for 12 h under anaerobic conditions at 25°C.

[0053] The alkynyl selenocystine (DBCO-SeCA) and PSLEQRTVYAKGGGGC-DBCO (abbreviated as DBCO-Peptide, the same below) were dissolved in PBS (PH=5) in a volume ratio of 0.01 mg / mL respectively: DMSO= In a 3:1 solution, the scaffold obtained...

Embodiment 2

[0055] The present embodiment provides a method for preparing a biological material with NO catalytic release and EPCs capture functions, which includes the following steps:

[0056] The titanium foil after heat treatment at 450°C was cleaned by oxygen plasma sputtering, the sputtering power was 300W, and the sputtering time was 10mins. A layer of TiO thin film was deposited on the surface of the material by magnetron sputtering. The deposition power was 600 W, the deposition time was 8 mins, and the film thickness was about 50 nm.

[0057] with N 2 After deoxygenation for 30 mins, PBS buffer with a pH of about 5 was used as a solvent, and the concentration was 0.01 mg / mL Ac-(DOPA)-Gly-(DOPA)-Lys(PEG5-Azido)-(DOPA)-Gly-(DOPA ) (abbreviated as ((DOPA) 4 -N 3 ), the same below), the scaffold was immersed in the solution and reacted for 12 h under anaerobic conditions at 25°C.

[0058] Alkynated selenocystine (DBCO-SeCA) and PSLEQRTVYAKGGGGC-DBCO (abbreviated as DBCO-Peptide,...

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Abstract

The invention discloses a biological material with functions of catalytic releasing NO and capturing EPCs and a preparation method thereof, and relates to the technical field of medical apparatus andinstruments. The preparation method includes steps of depositing a metal oxide film on the surface of a material to be modified, immersing the material in an azide o-phenol compound solution, and thenimmersing the material in a functionalized modification solution, specifically, the functionalized modification solution contains alkyne-based biomolecules capable of catalytic releasing NO and alkyne-based biomolecules with function of capturing EPCs. The prepared biological material comprises a substrate material, wherein the substrate material is sequentially deposited with a metallic oxide, the azide o-phenol compound and the alkyne-based functional biomolecules, and the functional biomolecules contain the biomolecules capable of catalytic releasing NO and the biomolecules with function of capturing EPCs. The material has the function of catalytic releasing NO and the function of capturing EPCs as well, so that the blood anticoagulation property of the material is improved, the smoothmuscle hyperplasia is inhibited, and the stent-in-situ re-endothelialization is promoted.

Description

technical field [0001] The invention relates to the technical field of medical devices, and in particular to a biological material with functions of NO catalytic release and EPCs capture and a preparation method thereof. Background technique [0002] The interventional treatment of vascular stents is currently the most widely used and most effective means of treating cardiovascular diseases in clinical practice. When stents are implanted in diseased vessels, it will lead to in-stent restenosis and late thrombosis. A healthy vascular endothelium is a natural anticoagulant tissue, which can maintain the normal contraction and relaxation of blood vessels, maintain smooth blood flow, and inhibit the formation of thrombus. Therefore, the surface of an ideal stent should have the function of promoting the regeneration of the normal endothelial cell layer, and fundamentally solve the problems of intravascular stent restenosis and late thrombosis. [0003] At present, the ways to a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C23C14/35C23C14/08C23C14/58A61L31/02A61L31/08A61L31/16
Inventor 杨志禄潘国庆郝蕊黄楠涂秋芬熊开琴
Owner SOUTHWEST JIAOTONG UNIV
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