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Biodegradable polymer containing phosphorylcholine and polyethylene glycol and synthetic method thereof

A polyethylene glycol, biodegradable technology, applied in the direction of transportation and packaging, packaging item types, special packaging items, etc., can solve the problem of reducing the adsorption capacity of protein molecules

Inactive Publication Date: 2009-09-23
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The initial study found that the adsorption of protein molecules on the surface of the MPC-co-BMA copolymer material was reduced due to the competitive adsorption of phospholipid molecules.

Method used

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  • Biodegradable polymer containing phosphorylcholine and polyethylene glycol and synthetic method thereof
  • Biodegradable polymer containing phosphorylcholine and polyethylene glycol and synthetic method thereof
  • Biodegradable polymer containing phosphorylcholine and polyethylene glycol and synthetic method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Dissolve 0.4g of MPC (the weight ratio of MPC is approximately the weight ratio of PC, the same below) and 0.6g of PEG-PLA with double bonds in chloroform, add a free radical polymerization initiator, and react at 60°C for 8 hours , the product was precipitated with methanol and dried in vacuo. PEG-PLA with a double bond is prepared by reacting PEG at one end with acryloyl chloride to initiate ring-opening polymerization of lactide. The reaction process is as follows:

[0025]

experiment example 1

[0026] Determination of Experimental Example 1 Polymer Structure

[0027] Experimental instrument: NMR

[0028] Experimental method: take by weighing the experimental sample of embodiment 1, dissolve in deuterium reagent, make 1 H-NMR test. figure 1 For Example 1 polymer 1 H-NMR spectrum, its characteristic peak -N(CH 3 ) 3 ,-OCH 2 CH 2 OPOCH 2 -, -CH 2 N corresponds to 3.212, 3.985-4.363, 3.792 in the figure respectively, and δ=3.643 is in PEG-(CH 2 CH 2 O) 45 -中-CH 2 - the characteristic peak, A 3.630 / A 3.982 =2.67 / 1.13, it can be calculated that MPC:PEG-PLA=38:1 in the copolymer, that is, the copolymer contains 34.8% (wt%) MPC.

[0029] Experimental Example 2 Measurement of Static Contact Angle

[0030] Experimental reagents: deionized water, ethylene glycol, chloroform

[0031] Experimental Instrument: Contact Angle Meter

[0032] Experimental method: dissolve the polymer of Example 1 in chloroform, and coat a film on a glass slide. After the chloroform v...

experiment example 3

[0033] Experimental Example 3 Platelet Adsorption Test

[0034] Experimental reagents: absolute ethanol, platelet enrichment solution (PRP), PBS buffer, glutaraldehyde solution

[0035] Experimental instrument: SEM

[0036] Experimental method: the coating film of Example 1 was immersed in PBS buffer solution, taken out after equilibrating at room temperature for 1 hour, and dried after taking out. Drop PRP on the sample to cover it, wash it with PBS solution for 3 times after 1 hour at 37 °C, then immerse the polymer film in glutaraldehyde solution for 1.5 hours, wash it with triple distilled water three times, and dehydrate it with anhydrous ethanol gradient. Observed by SEM after drying. image 3 Photograph of platelet adsorption. Although the surface of P(MPC-co-PEG-PLA) is not smooth, it can be seen that the number of platelets is very small, indicating that the combined effect of PEG and more PC groups has a significant effect.

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Abstract

The invention relates to a biodegradable polymer containing phosphorylcholine (PC) and polyethylene glycol (PEG) and a synthetic method thereof. The synthetic method comprises: MPC with the mass ratio of 1-99% and PEG-PLA which is connected with double linkage and has the mass ratio of 99-1% are dissolved in trichloromethane and then added with free radical polymerization initiator to react for 6-24h at the temperature of 0-80 DEG C, and the products are precipitated by methyl alcohol and dried in vacuum; the PEG-PLA which is connected with double linkage is prepared by ring opening polymerization of lactide initiated by the PEG that reacts with acryloyl chloride at a single end. The phosphorylcholine group which has positive and negative charges and is introduced on the PLA of the polymer can be seen according to the static contact angle result, and then the contact angle is obviously reduced, so that hydrophilicity is greatly improved. The higher the MPC content is, the smaller the contact angle is, and the better the wettability of the material is. Test results of anticoagulation prove that the PEG can reduce coagulation of blood platelet, affects prothrombin time (PT) and effectively avoids the activation of an extrinsic coagulation system; the high content PC group can effectively reduce the conglutination of the blood platelet and has influence on anginal partial thromboplastin time (APTT) of the activation part.

Description

technical field [0001] The invention relates to a biodegradable polymer containing phosphorylcholine (PC) and polyethylene glycol (PEG) and a synthesis method, which can be used for surface modification of anticoagulant materials or medical devices. Background technique [0002] The cell membrane actually constitutes an ideal, non-coagulant interface, and the acquisition of such a non-coagulant interface comes from the perfect combination of specific physical and chemical properties of the cell membrane. Since the interaction between cells and receptors is very complex, and the signal transduction related to cell adhesion and proliferation inside the cell is also very complicated, so far, the work in this area has yet to be further improved, but it has shown good development prospect. Another potential alternative for endothelialization on artificial device surfaces is to construct a biomimetic surface that mimics cell membranes on the surface of medical devices. Since the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F290/06A61L33/06
Inventor 马桂秋原续波何斌刘霞盛京
Owner TIANJIN UNIV
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