Preparation method of composite nanometer fiber small-diameter intravascular tissue engineering stent material

A technology of composite nanofibers and vascular tissue, used in stents, medical science, prostheses, etc., can solve problems such as the inability to simulate the structure and function of the extracellular matrix, the difficulty of determining the elasticity and compliance of the construct, and the residual stent. Achieving good biocompatibility and anticoagulant properties, favorable adhesion and growth, good mechanical properties

Inactive Publication Date: 2010-02-24
FUJIAN NORMAL UNIV
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  • Abstract
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Problems solved by technology

However, the disadvantage of the casting-dipping method is that toxic solvents remain in the scaffold and the size and distribution of the pores cannot be well controlled; the cell self-assembly technology requires high preparation conditions and must be operated under sterile conditions. The long-term culture required for the constructed blood vessels is also considerably limited, and it is difficult to determine whether the constructs exhibit suitable elasticity and compliance after implantation; although the gel spinning method can prepare miniature vascular stents, the stent’s Microstructure cannot well mimic the structure and function of the extracellular matrix

Method used

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  • Preparation method of composite nanometer fiber small-diameter intravascular tissue engineering stent material
  • Preparation method of composite nanometer fiber small-diameter intravascular tissue engineering stent material
  • Preparation method of composite nanometer fiber small-diameter intravascular tissue engineering stent material

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Embodiment 1

[0028] 1. Preparation of electrospinning solution: Weigh and dissolve 0.1g pNSR16, 0.8gPCL and 0.1gCS in 5ml of 88% formic acid to obtain 20% (w / v) pNSR32 / PCL / CS mixed electrospinning solution.

[0029] 2. Shaft treatment: Coat a thin layer of polyethylene glycol (PEG) on the stainless steel shaft.

[0030] 3. Preparation of small-diameter vascular stent: draw 1.5ml of spinning solution into a 2ml syringe, place the syringe on the syringe pump, use a cylindrical shaft collector with a cross-sectional diameter of 4mm at a speed of 1000r / min Under the conditions of rotation, curing distance of 18cm, voltage of 80kV, extrusion speed of 5ml / h, and temperature of 45°C, a pNSR32 / PCL / CS composite nanofiber small diameter vascular stent with an inner diameter of 4mm was prepared.

[0031] 4. Post-processing of stent preparation: After rinsing the stent with PBS, immersing in 70% alcohol for 20 minutes, sterile water for 3 minutes and sterile PBS for 5 minutes, the residual polyethylene glyco...

Embodiment 2

[0033] 1. Electrospinning solution preparation: Weigh and dissolve 0.15g pNSR32, 1.2gPCL and 0.15gCS in 5ml 98% formic acid to obtain 30% (w / v) pNSR32 / PCL / CS mixed electrospinning solution.

[0034] 2. Shaft treatment: Coat a thin layer of polyethylene glycol (PEG) on the stainless steel shaft.

[0035] 3. Preparation of small-diameter vascular stent: Draw 2.0ml of spinning solution into a 2.5ml syringe, place the syringe on the syringe pump, and use a cylindrical shaft collector with a cross-sectional diameter of 6mm, with a speed of 1500r / min Under the conditions of speed rotation, curing distance of 20cm, voltage of 100kV, extrusion speed of 7ml / h, and temperature of 50℃, a pNSR32 / PCL / CS composite nanofiber small diameter vascular stent with an inner diameter of 6mm was prepared.

[0036] 4. Post-processing of stent preparation: After rinsing the stent with PBS, immersing in 70% alcohol for 20 minutes, sterile water for 3 minutes and sterile PBS for 5 minutes, the residual polyeth...

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Abstract

The invention relates to the field of tissue engineering, in particular to a preparation method of a composite nanometer fiber small-diameter intravascular tissue engineering stent material. The technical scheme is achieved as follows: formic acid is used as a common solvent; RGD-recombinant spider silk protein, polycaprolactone and chitosan are mixed in a mass ratio of 1:8:1 according to the requirements of an intravascular stent to prepare a spinning solution with the concentration of 20-30 percent (w/v); electrospinning process parameters are as follows: the diameter of a cross section of acylindrical rotating shaft is 3-6 mm, the rotating speed is 1,500-3,000rpm, the curing distance is 15-30 cm, the voltage is 50-150kV, the extrusion speed is 3-7ml/h and the temperature is 45-50 DEG C. The small-diameter intravascular stent has favorable cell adhesion-promoting capability, anticoagulant property and mechanical property for resisting physiological environment and can be clinicallyapplied as a small-diameter intravascular tissue engineering stent.

Description

Technical field [0001] The invention relates to the field of tissue engineering, in particular to a method for preparing a composite nanofiber small-diameter vascular tissue engineering scaffold material. Background technique [0002] Artificial synthetic blood vessels and autologous blood vessels are the main sources of small diameter vascular grafts in clinical practice. In practice, artificial synthetic blood vessels have problems such as thrombosis, intimal hyperplasia, and low long-term patency after implantation; autologous blood vessels are limited by source and size, and cannot meet the needs of small-diameter vascular grafts. Vascular tissue engineering provides an effective way to solve the source of small-diameter vascular grafts. Its basic principle is to co-culture normal vascular cells with biodegradable scaffold materials to form vascular tissues with specific morphology and functions to repair lesions and wounds. And the purpose of rebuilding the function. The c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/58A61F2/82A61L27/22A61L27/20A61L27/18
Inventor 薛正翔陈登龙李敏
Owner FUJIAN NORMAL UNIV
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