Method applicable to in-vitro mature culture of young rabbit oocytes and formulation of culture medium

A technology for in vitro maturation and culture of oocytes, which is applied to the field of in vitro maturation and culture of young rabbit oocytes, and can solve the problem that young animal oocytes are rare.

Inactive Publication Date: 2012-09-19
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still relatively few reports on the in vitro maturation of young animal oocytes.

Method used

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  • Method applicable to in-vitro mature culture of young rabbit oocytes and formulation of culture medium
  • Method applicable to in-vitro mature culture of young rabbit oocytes and formulation of culture medium
  • Method applicable to in-vitro mature culture of young rabbit oocytes and formulation of culture medium

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] 1. Experimental animals Healthy 60-day-old young New Zealand white rabbits were selected as the experimental rabbits (preliminary experiments have proved that 60-day-old is the best superovulation age for young rabbits).

[0026] 2. Experimental grouping Maturity fluid basal culture system: add 10% FBS, 0.11g / L sodium pyruvate, 10μg / ml LH, 10μg / ml FSH, 1μg / ml E to TCM-199 2 , 100IU / ml penicillin, 100IU / ml streptomycin sulfate. The experiment was divided into five groups: Group I was the control group, and Groups II, III, IV, and V were the test groups.

[0027] Ⅰ: Basal culture medium + 0 μmol / L EGCG

[0028] Ⅱ: Basal culture medium + 10μmol / L EGCG

[0029] Ⅲ: Basal culture medium + 15μmol / L EGCG

[0030] Ⅳ: Basal culture medium + 20μmol / L EGCG

[0031] Ⅴ: Basal culture medium + 25 μmol / L EGCG

[0032] 3. Superovulation treatment of young rabbits Young rabbits were injected intramuscularly with FSH twice a day, with an interval of 12 hours, for 3 consecutive days, ...

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Abstract

The invention belongs to the field of biology and particularly relates to a method applicable to in-vitro mature culture of young rabbit oocytes and a formulation of a culture medium. According to the method, a cumulus-oocytes complex is cultured in vitro by using a TCM-199 basic culture system; based on a TCM-199 culture medium, 10% of FBS, 0.11 g/L of sodium pyruvate, 10 Mug/ml of LH, 10 Mug/ml of FSH, 1 Mug/ml of E2, 100 IU/ml of penicillin, 100 IU/ml of streptomycin sulfate and 15-20 Mum of epigallocatechin gallate (EGCG). According to the invention, the EGCG is added into the TCM-199 culture medium to study the influence of the EGCG on in-vitro maturation of young New Zealand rabbit oocytes, so that a simple and practical method for in-vitro maturation of the young rabbit oocytes is established.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a method suitable for in vitro maturation and culture of young rabbit oocytes. Background technique [0002] JIVET technology (juvenile in vitro embryo transfer) is a biological high-tech system integrating superovulation and egg collection of young animals, in vitro maturation of oocytes, in vitro fertilization, in vitro culture of embryos and embryo transfer. It was first established in South Australia, Australia Research and development by the government's Reproduction and Development Research Center (South Australia Reproduction Institute, SARD). Using young animals as donors to perform superovulation on young animals can not only provide a large number of oocyte resources for in vitro production of embryos, meet the needs of people's scientific research and production, but also greatly shorten the intergenerational interval, speed up the breeding process, and It provides ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/075
Inventor 李拥军李太坤李文婷张光景张佳慧
Owner YANGZHOU UNIV
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