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Absorbable antibacterial hemostatic microsphere, preparation method and application thereof

A technology of microspheres and lysozyme, which is applied in the fields of application, pharmaceutical formulation, and medical science, can solve the problems of unsuitable hemostatic powder, difficult to degrade and absorb, etc., and achieve the effects of long storage time, convenient use, and fast degradation speed

Inactive Publication Date: 2016-09-28
ZHEJIANG SCI-TECH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of material hopes to use halloysite and chitosan to play the respective advantages of inorganic and organic materials to play a hemostatic and bactericidal effect, but as it says, halloysite nanotubes are inorganic materials, and the main components are silicon oxide, aluminum oxide and a small amount of other metals Oxides, such ingredients are difficult to be degraded and absorbed in the body, and cannot be hydrolyzed by lysozyme to exert a synergistic effect, so it is not suitable as a hemostatic powder for hemostasis in vivo

Method used

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  • Absorbable antibacterial hemostatic microsphere, preparation method and application thereof
  • Absorbable antibacterial hemostatic microsphere, preparation method and application thereof
  • Absorbable antibacterial hemostatic microsphere, preparation method and application thereof

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Embodiment 1

[0036] 1. Preparation of matrix solution: Add 1.5 g of sodium alginate, 0.5 g of carboxymethyl chitosan and 0.025 g of gelatin into 100 ml of water and dissolve to make a matrix solution; 2. Mixing of materials: 250 ml of liquid paraffin and 18 ml of spit Mix at 80°C, add matrix solution, and 10 ml of 2% w / w calcium chloride, accompanied by rapid stirring, to carry out cross-linking reaction to prepare microspheres. 3. Refining: After the material liquid is separated, pour out the upper oil phase, discard it, add 500ml of absolute ethanol to the lower liquid, stir, remove the supernatant, and repeat washing 2 to 3 times. Suction filter, add 30 ml of distilled water to dilute and spray dry to obtain microspheres of about 5-60 μm. Add 0.01 g of lysozyme to the microspheres, blend with air blowing, vacuum dry, aseptically treat and seal the package.

Embodiment 2

[0038] 1. Preparation of matrix solution: Add 1.5 g sodium alginate, 0.5 g carboxymethyl chitosan and 0.025 g gelatin to 100 ml water to dissolve to make matrix solution; 2. Material mixing: 100 g fatty acid and 18 ml Tween Mix at 80, add matrix solution, and 10 ml of 2% w / w calcium chloride, accompanied by rapid stirring, to carry out cross-linking reaction to prepare microspheres. 3. Refining: After the material liquid is separated, pour out the upper oil phase, discard it, add 500ml of absolute ethanol to the lower liquid, stir, remove the supernatant, and repeat washing 2 to 3 times. Suction filtration, adding 30 ml of distilled water to dilute and spray-dry to obtain microspheres of about 5-60 μm, vacuum-dried, aseptically treated, and then sealed and packaged (no lysozyme, blank control group).

Embodiment 3

[0040] 1. Preparation of matrix solution: Add 1.5 g of sodium alginate, 0.5 g of carboxymethyl chitosan and 0.025 g of gelatin to 100 ml of water and dissolve to make a matrix solution; 2. Mixing of materials: 200 ml of castor oil and 18 ml of spit Mix at 80°C, add matrix solution, and 10 ml of 2% w / w calcium chloride, accompanied by rapid stirring, to carry out cross-linking reaction to prepare microspheres. 3. Refining: After the material liquid is separated, pour out the upper oil phase, discard it, add 500ml of absolute ethanol to the lower liquid, stir, remove the supernatant, and repeat washing 2 to 3 times. Suction filter, add 50 ml of 5% ethanol to dilute and spray dry to obtain microspheres of about 5-60 μm. Add 0.01 g of lysozyme to the microspheres, blend with air blowing, vacuum dry, aseptically treat and seal the package.

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Abstract

The invention relates to an absorbable antibacterial hemostatic microsphere, a preparation method and application of the absorbable antibacterial hemostatic microsphere. The absorbable antibacterial hemostatic microsphere is prepared from carboxymethyl chitosan, sodium alginate, gelatin and lysozyme. The absorbable antibacterial hemostatic microsphere disclosed by the invention can be used for carrying out quick hemostasis on a massive haemorrhage wound and preventing the massive haemorrhage wound from being infected by bacteria; the absorbable antibacterial hemostatic microsphere has good biocompatibility, can be degraded and absorbed and is capable of promoting the wound healing. The invention also discloses the effect of the application of the composite absorbable antibacterial hemostatic microsphere in hemostasis of New Zealand rabbit hemihepatectomy.

Description

technical field [0001] The invention relates to an absorbable antibacterial hemostatic microsphere and a preparation method thereof. Background of the invention [0002] Hemostatic dressings have the largest variety of hemostatic agents and are most widely used. Among them, dry fibrin dressings, zeolite dressings, and chitosan dressings are particularly representative. [0003] Dry fibrin dressings are made primarily by adding fibrin and thrombin to gauze to improve the hemostatic effect of standard gauze. The mechanism of hemostasis is to provide exogenous substrates for the coagulation reaction. The material composition can be absorbed, and the hemostatic effect is stable, but the cost is expensive, and the price is 500-1000 US dollars. The source of ingredients, the fragility of the material itself, the problem of microbial inactivation technology and the high price limit the application of this type of dressing. [0004] Zeolite dressing is mainly made of molecular s...

Claims

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Application Information

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IPC IPC(8): A61L24/04A61L24/00
CPCA61L24/001A61L24/0015A61L24/0031A61L24/043A61L2300/254A61L2300/418A61L2300/404A61L2400/04
Inventor 金甲杨玉民叶飞
Owner ZHEJIANG SCI-TECH UNIV
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