Method for establishing animal mode of blood vessel sextual erection dys function

A technology of erectile dysfunction and animal models, applied in the fields of medicine and animal behavior, which can solve the problems of animal models without vascular ED

Inactive Publication Date: 2005-12-28
RENJI HOSPITAL ATTACHED TO SHANGHAI NO 2 MEDICALUNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Taking rabbits as experimental animals, if they are fed with high-cholesterol and high-fat feed at the same time, atherosclerosis can occur, but the problem is that this kind of pathological change is mainly due to the formation of atherosclerotic plaques in the aorta of rabbits, and the main The iliac artery, the blood vessel supplying the penis, is difficult to form atherosclerosis
Therefore, so far there is no satisfactory animal model of vascular ED

Method used

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  • Method for establishing animal mode of blood vessel sextual erection dys function
  • Method for establishing animal mode of blood vessel sextual erection dys function
  • Method for establishing animal mode of blood vessel sextual erection dys function

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Experimental program
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Effect test

Embodiment 1

[0043] The preparation of embodiment 1 experimental animal and the preparation of animal model

[0044] All New Zealand rabbits were housed in separate cages at an indoor temperature of 15-25°C. They were fed with conventional pellet feed for rabbits every day, once in the morning and once in the evening, and had free access to drinking water.

[0045] All 36 male New Zealand rabbits were randomly divided into two groups A and B, each with 18 rabbits. The two groups were randomly divided into high fat model group (A1 and B1) and control group (A2 and B2). The control group was always fed with conventional pellet feed; the model group was fed with cholesterol at a ratio of 1%-1.5% every day, and lard at a ratio of 4%-6%. feed. 5-7 days after the first feeding, the model group underwent bilateral balloon catheter dilatation of the internal iliac artery to damage the internal iliac artery endothelium. All animals were detected and identified on the 25th-30th day and the 50th-60...

Embodiment 2

[0056] Embodiment 2 mating experiment

[0057] The mating experiments were carried out in the 4th and 8th weeks after operation. The experimental animals were placed in self-made observation cages, the room was kept quiet, and the lights were slightly dimmed. After acclimating to the environment for a few minutes, gently put the female rabbit in estrus into the observation cage. Each male rabbit was observed for 30 minutes or until the first ejaculation. The observed parameters include: (1) mount latency (ML): the time required for the female rabbit to climb up for the first time in the same cage; (2) mount frequency (MF): climb before ejaculation High frequency, regardless of whether there is insertion or not; (3) insertion frequency (IF): the number of insertions before ejaculation; (4) hit rate (hit rate, HR): the ratio of the number of insertions to the number of climbs.

[0058] The mating conditions of each group of rabbits and female rabbits in estrus are shown in Ta...

Embodiment 3

[0061] Embodiment 3 rabbit penis blood flow 99m Technetium- 113m Indium dual nuclide detection

[0062] The dual-nuclides detection was carried out after the 4th postoperative or (Group A) 8th week (Group B) after the mating experiment ( Figure 5 ). The anesthesia method is the same as above. According to the preliminary experiment, generally New Zealand white rabbits can induce normal penile erection with 2-4mg papaverine hydrochloride. Therefore, 5 mg papaverine hydrochloride was used to induce penile erection in the treatment group and the control group rabbits. All experimental animals were injected into the corpus cavernosum The active drug volume is 0.17ml. See [Chen Bin et al., 99m Tc- 113m Application of In dual-nuclides technique in the diagnosis of vascular erectile dysfunction, Chinese Journal of Andrology 2000; 14(4): 297]. The main outcome measures were arterial imaging index (PIA) and venous imaging index (PIV).

[0063] At the 4th week, when the dual ra...

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Abstract

A method for setting up animal model of vascular erection dysfunction includes using New Zealand rabbit to add effective amount of cholesterol and lard in its feedstuff, feeding this for certain period then executing dilatation plasty of internal iliac artery at two sides of saccule catheters to damage its internal skin of internal iliac artery, carrying out detection and identification by mating test, dicaryon or injecting blood active medicine on cavernous body after operation.

Description

technical field [0001] The invention belongs to the fields of medicine and animal behavior, and relates to a method for establishing an animal model. Further, the invention relates to a method for establishing an animal model of vascular erectile dysfunction. Background technique [0002] Erectile dysfunction (ED) has always been a hot spot in urology and andrology. For a long time, it was believed that the causes of ED were primarily psychological. With the advancement of research methods and the collection of various clinical data, it has been found that most EDs have various organic causes. Because the etiology of ED is complex, so far, the pathogenesis of ED is still unclear, so finding a suitable animal model of ED is necessary for the occurrence, development, clinical prevention and development of new drugs of ED. [0003] Hyperlipidemia is one of the common diseases of middle-aged and elderly people. Current studies have found that with the improvement of living st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G09B23/28
Inventor 陈斌
Owner RENJI HOSPITAL ATTACHED TO SHANGHAI NO 2 MEDICALUNIV
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