The invention discloses an external preparation for preventing and treating male sexual dysfunction. The external preparation comprises mesenchymal stem cell supernatant, water-soluble laurocapram, arginine, taurine, peppermint oil, small molecular oyster peptide, ginsenoside extract, yohimbine extract, lubrajel and double distilled water. Every 200ml of finished product comprises the following components in percentage by volume/volume: 15% of mesenchymal stem cell supernatant, 2% of water-soluble laurocapram, 2% of peppermint oil and 6% of lubrajel. The external preparation further comprisesthe following components in percentage by mass/volume: 5% of arginine, 5% of taurine, 2.5% of small molecular oyster peptide, 2% of a ginsenoside extract and 2% of a yohimbine extract, and the balanceof double distilled water which is quantified to 200ml. The external preparation can promote the blood sinus filling of the cavernous body of the penis, has a direct effect on the aspects of erectionassistance, time delay, thickening and the like, and has the advantages of quick response, good curative effect and high safety. The externally-applied smearing formula reduces the risk caused by oral administration and penis local drug injection.