Method for establishing animal model infected with Acinetobacter baumannii pneumonia

Abstract

The invention discloses a method for establishing an animal model infected with Acinetobacter baumannii pneumonia. The method comprises the following steps: (1) preparing an immunosuppressive New Zealand rabbit; (2) preparing an Acinetobacter baumannii solution; (3) infecting the animal and establishing the model. The simple, rapid and efficient animal model infected with Acinetobacter baumannii pneumonia is established to help people research production and development processes of drug resistance of Acinetobacter baumannii and drug resistance related mechanisms of the Acinetobacter baumannii, assistance is provided for delaying of drug resistance of bacteria and better treatment of infection with the Acinetobacter baumannii, expense for related treatment of patients can be reduced, and human health can be promoted.

Description

technical field [0001] The invention relates to the technical field of medical animal models, in particular to a method for constructing an animal model of Acinetobacter baumannii pneumonia. Background technique [0002] In recent years, domestic and foreign reports on Acinetobacter baumannii have gradually increased. According to Keen and Robinson's report, the detection rate of Acinetobacter baumannii ranks first, as high as 53%. Studies by Mendes et al. have shown that the detection rate of Acinetobacter has been increasing year by year in recent years, and infections caused by Acinetobacter baumannii account for 80%-90% of Acinetobacter. In 2013, China CHINET Bacterial Resistance Surveillance showed that the detection rate of Acinetobacter baumannii in the hospital was as high as 11.97%, ranking third among all Gram-negative bacteria, and showing a trend of high drug resistance and multi-drug resistance , the resistance rates to imipenem and meropenem were 62.8% and 59...

AI Technical Summary

Problems solved by technology

The advantage is that the method is simple and easy to operate, but the disadvantage is that some parasitic bacteria in the respiratory tract may enter the lower respiratory tract, which will interfere with the experimental results; nasal drip is likely to cause sneezing reflex in animals, and the bacterial suspension is not easy to quantify bacteria, resulting in individual differences; and Nasal instillation has a high concentration of bacterial solution, which is more irritating to the airway mucous membrane than aerosol inhalation

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