Bacillussubtilis for decomposing aflatoxin and active protein secreted by same

A technology of Bacillus subtilis and aflatoxin, applied in the direction of microorganism-based methods, medical raw materials derived from bacteria, application, etc., can solve the problem of simultaneous degradation of aflatoxin B1 by Bacillus subtilis, the degradation efficiency is not high, and has not yet been found Degradation and other problems, to achieve good probiotics and stress resistance, high detoxification activity, specific effects

Active Publication Date: 2011-04-27
CHINA AGRI UNIV
View PDF2 Cites 33 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented bacilli are effective at reducing harmful substances from crops such as wheat or barley grapefruit (Citrus reptans). They also help improve food safety without affecting their taste qualities like flavor. These microorganisms produce enzymes that break down certain compounds found naturally occurring within crop plants called phytotrienols which cause them' symptoms including reduced growth rates due to lack of oxygen supply during storage periods. Additionally, these bacteria provide benefits related to improving digestibility when fed into animals through ingestion.

Problems solved by technology

This patent describes various ways for controlling the amount of certain substances present in crops like wheat germ meals without causing negative side impacts on animals and plants themselves. However, current techniques involve expensive processes involving multiple steps, which may result in reduced productivity due to increased risk from pesticides used in agricultural products.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillussubtilis for decomposing aflatoxin and active protein secreted by same
  • Bacillussubtilis for decomposing aflatoxin and active protein secreted by same
  • Bacillussubtilis for decomposing aflatoxin and active protein secreted by same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 The cultivation method of Bacillus subtilis of the present invention

[0065] Take Bacillus subtilis ANSB324 CGMCC No. 36091.5ml (the concentration of viable bacteria is 10 9 CFU / ml), inoculated in 80ml medium for shaking flask fermentation culture, fermentation temperature is 37℃, pH value is 7.0, rotation speed is 200r / min, and fermentation time is 24h.

[0066] Among them, the shake flask fermentation medium consists of the following components: tryptone 10g, yeast extract 2g, glucose 2g, beef extract 3g, sodium chloride 4g, disodium hydrogen phosphate 3g, magnesium sulfate heptahydrate 1.0g, manganese sulfate 1.0g, 1000mL distilled water, pH 7.2.

[0067] After fermentation, the fermentation broth is stored in a refrigerator at 4°C for later use.

Embodiment 2

[0068] Example 2 The cultivation method of Bacillus subtilis of the present invention

[0069] Take Bacillus subtilis ANSB324 CGMCC No. 36090.5ml (the concentration of viable bacteria is 10 9 CFU / ml), inoculated in 50ml medium for shaking flask fermentation culture, fermentation temperature is 30℃, pH value is 7.0, rotation speed is 180r / min, and fermentation time is 20h.

[0070] Among them, the shake flask fermentation medium consists of the following components: tryptone 8g, yeast extract 1.5g, glucose 1.5g, beef extract 2g, sodium chloride 3g, disodium hydrogen phosphate 2.5g, and magnesium sulfate heptahydrate 0.5g , 1.0g manganese sulfate, 800mL distilled water, pH 7.0.

[0071] After fermentation, the fermentation broth is stored in a refrigerator at 4°C for later use.

Embodiment 3

[0072] Example 3 The cultivation method of Bacillus subtilis of the present invention

[0073] Take Bacillus subtilis ANSB324 CGMCC No. 36092.5ml (the concentration of viable bacteria is 10 9 CFU / ml), inoculated in 100ml medium for shaking flask fermentation culture, fermentation temperature is 40℃, pH value is 7.4, rotation speed is 220r / min, and fermentation time is 30h.

[0074] Among them, the shake flask fermentation medium consists of the following components: tryptone 12g, yeast extract 3g, glucose 4g, beef extract 5g, sodium chloride 6g, disodium hydrogen phosphate 4g, magnesium sulfate heptahydrate 1.5g, manganese sulfate 1.5g, 1200mL distilled water, pH 7.4.

[0075] After fermentation, the fermentation broth is stored in a refrigerator at 4°C for later use.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a bacillussubtilis (Bacillussubtilis ANSB324 CGMCC No.3609) for decomposing aflatoxin, protein secreted by the same and an application thereof. The invention also provides a method for decomposing aflatoxin B1, G1 and M1 by using fermentation liquor of the strain or the protein secreted by the strain. By the method, after the bacillussubtilis and the aflatoxin B1 react for 48 hours, the decomposition rate of the B1 reaches 85%; after the bacillussubtilis and the aflatoxin G1 react for 72 hours, the decomposition rate of the G1 reaches about 66%; and after the bacillussubtilis and the aflatoxin M1 react for 72 hours, the decomposition rate of the M1 reaches about 68%. For the bacillussubtilis and the active protein secreted by the bacillussubtilis, the detoxifying activity is high, the specificity is strong, the action effect is mild, therefore, the bacillussubtilis provided by the invention is applicable to decomposing and removing the aflatoxin in feedstuff. Simultaneously, the bacillussubtilis provided by the invention has the advantages of strong bioactivity, good probiotics and stress resistance, and the like, and does not damage nutrient components in the feedstuff.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap