Screening method of banana aquaporin gene promoter transcription factor

A technology of channel protein and transcription factor, which is applied in the screening field of banana aquaporin gene promoter transcription factor, can solve the problems of reduced photosynthetic efficiency, influence of flower bud differentiation, yellowing and withering, etc., and achieves the effect of facilitating research and improving drought resistance

Pending Publication Date: 2019-09-13
HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI +1
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Problems solved by technology

When bananas encounter drought during the vegetative growth stage, the growth of banana vegetative organs will be stunted, and the growth rate and growth volume will be significantly reduced. Drought ...

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  • Screening method of banana aquaporin gene promoter transcription factor

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Embodiment Construction

[0014] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments.

[0015] refer to figure 1 , a screening method for a banana aquaporin gene promoter transcription factor, comprising the following steps:

[0016] 1. Promoter cloning and element analysis

[0017] The primers of the promoter were designed according to the banana A genome website, and the amplification length was 1362bp. Its cis-acting elements were analyzed by plantcare and PLACE.

[0018] The results show that there are 72 cis-acting elements in the 1362bp promoter sequence, including many TATA-box and CAAT-box core elements, including ABA response elements ABRE, MYB elements, MYC elements, ERE elements, MeJA response elements, BOX II, ...

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Abstract

The invention discloses a screening method of a banana aquaporin gene promoter transcription factor. The screening method includes the following steps: a core region of a promoter is judged, wherein according to a banana A genome, primers of the promoter are designed and amplified, the amplification length is 1362 bp, through plantcare and PLACE, cis-acting elements of the promoter are analyzed, GUS activity of the promoter is detected, and through dyeing conditions, the core region B of the promoter is judged; and yeast one-hybrid is conducted to screen the transcription factor interacting with the promoter, wherein the yeast one-hybrid method is used for verification, the core region B of the promoter serves as a bait section for building a bait carrier, and a banana one-hybrid library obtained through yeast drought treatment is screened. By adopting the screening method of the banana aquaporin gene promoter transcription factor, the transcription factor which is directly bonded to the MaPIP1;1 promoter under drought stress conditions is identified, therefore, research for banana aquaporin genes is more convenient, the draught resistance capability of transgenic bananas can be improved, and a draught resistance mechanism of MaPIP1;1 can be further understood.

Description

technical field [0001] The present invention relates to the technical field of promoter transcription factors, in particular to a screening method for the promoter transcription factors of banana aquaporin genes. Background technique [0002] In the early stage of banana, the plant is small, the root system is shallow, and it is susceptible to drought. Its leaf area is large and the amount of transpiration is large, especially in the high temperature season. The earth damages the normal physiological and metabolic activities of bananas, resulting in reduced production and quality of bananas. In banana cultivation and production, water consumption is large, and every 500 grams of dry matter produced requires 300 kilograms of water to be absorbed. In the vegetative growth stage of bananas, drought will cause poor growth and development of banana vegetative organs, and the growth rate and amount of growth will be significantly reduced. When the drought is severe, the leaves wi...

Claims

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Application Information

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IPC IPC(8): C12Q1/6897C40B30/04
CPCC12Q1/6897C40B30/04
Inventor 许奕宋顺李敬阳金志强徐碧玉王安邦李羽佳黄东梅魏卿
Owner HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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