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Establishing method of high-casein ration-induced type post-weaning piglet nutritional diarrhea model

A technology for weaning piglets and establishing methods, which is applied to weighing equipment, measuring devices, instruments and other directions of materials with special properties/forms, can solve problems such as insufficient protease secretion and low protein digestion ability, and achieve obvious diarrhea characteristics and digestion. High rate and good repeatability

Inactive Publication Date: 2019-04-23
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since most animal feeds require plant protein and anti-nutritional factors, when piglets are weaned, their digestive system is generally not fully developed, protease secretion is insufficient, and their ability to digest protein is extremely low

Method used

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  • Establishing method of high-casein ration-induced type post-weaning piglet nutritional diarrhea model
  • Establishing method of high-casein ration-induced type post-weaning piglet nutritional diarrhea model
  • Establishing method of high-casein ration-induced type post-weaning piglet nutritional diarrhea model

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Experimental program
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Effect test

Embodiment Construction

[0028] The method for establishing the nutritional diarrhea model of weaned piglets induced by high casein diet is characterized in that it comprises the following steps:

[0029] Step 1. Experimental animals and grouping: Select 14 weaned piglets at 21 days old, with a body weight of 7.98±0.14kg, and randomly divide them into two groups: control group A and high-protein group B, with 7 pigs in each group, and the control group is fed with crude protein Level is 17% diet, high protein group is fed with crude protein level is 30% diet, turn to step two; control group A and high protein group B diet formulations are shown in Table 1, control group and experimental group grouping situation See Table 2.

[0030] Table 1 Experimental diet formula (this formula is a semi-homogeneous diet)

[0031]

[0032] The premix provides per kg feed: sepiolite, 6.043g; pig vitamins, 750mg; iron, 150mg; copper, 150mg; manganese, 80mg; zinc, 120mg; selenium, 0.3mg; cobalt, 1mg; iodine , 0.3m...

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Abstract

The invention relates to a post-weaning piglet nutritional diarrhea model, specifically relates to the high-casein ration-induced type post-weaning piglet nutritional diarrhea model, and belongs to the field of establishment of animal models. A establishing method of the high-casein ration-induced type post-weaning piglet nutritional diarrhea model is characterized in that a 21-day post-weaning piglet is continuously fed for a high-casein ration for 15 days, casein in the high-casein ration is a unique source of protein, the adding amount of the casein is 33.72%, and the content of crude protein in the ration is 30%; and after a test is finished, a piglet diarrhea index, a serum biochemical index, an intestinal structure form and the expression amounts of an intestinal water channel protein marker molecule and a tight junction protein marker molecule in the piglet are measured. The establishing method involved in the invention has the beneficial effects that the casein is used as the unique source of the protein, the adding amount of the casein in the ration is determined, and the high-casein ration-induced type post-weaning piglet nutritional diarrhea model is established, so thata reliable model is provided for the deep exploration and prevention of post-weaning diarrhea of newborn animals.

Description

technical field [0001] The invention belongs to the field of animal model construction, and relates to a weaned piglet nutritional diarrhea model, in particular to a method for establishing a weaned piglet nutritional diarrhea model induced by a high-casein diet. Background technique [0002] my country has always been a big country in the pig industry, with roughly 690 million pigs being slaughtered every year. However, in modern pig production, weaning places enormous stress on piglet growth and is accompanied by significant changes in gastrointestinal physiology, microbiology and immunology. As a result of these changes, piglets have intestinal disturbances for a period of time after weaning, reduced growth performance, and are highly prone to post-weaning diarrhea. Growth retardation and intestinal diseases caused by post-weaning diarrhea are a major test for piglets during weaning, and even cause death of piglets, causing serious economic losses to the pig industry and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A23K50/60A23K50/30A23K20/147G01G17/08G01N33/50
CPCA01K67/02A23K20/147A23K50/30A23K50/60G01G17/08G01N33/50
Inventor 高晶李铁军印遇龙
Owner INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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