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Cultivated silkworm microsporidian polar tube protein PTP1 gene

A technology of microsporidia and polar silk protein, applied in genetic engineering, plant genetic improvement, animal/human peptides, etc., to achieve the effect of aggravating disease and accelerating death

Inactive Publication Date: 2008-03-12
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on the polar tube protein of Microsporidia is limited to a small number of Microsporidia such as Encephalitozoon, Glugea, Ameson and Nosema.

Method used

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Embodiment

[0074] Using Glimmer3.0 software combined with homology comparison to predict the genes of N. silkworm, and analyze the arrangement of genes on the genomes of N. silkworm and N. rabbit encephalitis. It is found that the arrangement of homologous genes on the genome has Strong collinearity, and the coded microsporidia polar tube protein PTP1 of Bombyx mori was obtained; a pair of specific primers were designed using Primer5.0 primer design software, respectively PTP1-F5, CGGGATCCATGGCATGTTCACCCGGAAAT-3′ and PTP1-R5′CGGTCGACGTTTAGCACACATGGATTATTGGC- 3. The amplified fragment was cloned into the pMD18-T vector. The sequence determination was completed by Shanghai Yingjun. The homology comparison was completed by BLAST on the NCBI site. Sequence analysis showed that it was a new protein gene.

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Abstract

The invention provides an important protein gene PTP1 relative with growth and infection of microsporidia of silkworms. The gene sequence CDS for coding the pole-tube protein PTP1 for microsporidia of silkworms is got based on the 9-magification gene data of microsporidia of silkworm and by collinear analysis. The pole-tube protein PTP1 got by the invention is of high importance for studying the breeding and infection mechanism of microsporidia, hence can play role in preventing and treating microsporidia in production practice. According RNAi interference, after the pole-tube protein PTP1 being interfered, the microsporidia loses the capability for infecting the host. The pole-tube protein PTP1 and its potential controlling gene of microsporidia will become a new factor for treating microsporidia infection. In addition, by means of transgenosis, the expression of the water-channel protein gene can be enriched, so that the water-channel protein gene can be used to control such destructive insects as locusts, etc.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a PTP1 gene derived from the silkworm microsporidia extreme silk protein related to the reproduction and infection of the silkworm microsporidia. Background technique [0002] Microsporidia are intracellular obligate parasitic protozoa commonly distributed in nature. There are nearly 150 genera and more than 1,200 species. They have a very wide range of hosts, including vertebrates and invertebrates. They are economic insects, fish, rabbits, hair-producing Deadly pathogen of animals, rodents and primates. Nosema bombycis belongs to the phylum Protozoa, the subphylum Monofilamenta, and the class Microsporidia in taxonomic status. Microsporidium parasitizes the economic insect Bombyx mori and causes severe infectious microparticle disease, and because Microsporidium can be transmitted to the next generation through the embryo, it has been listed as the legal quarantine object of seric...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/31C07K14/37C12N15/30C07K14/44
Inventor 周泽扬潘国庆李春峰向仲怀
Owner SOUTHWEST UNIV
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