Microelectronic sensor device for DNA detection

A sensor device, microelectronics technology, applied to measuring devices, laboratory containers, instruments, etc., can solve problems such as sample research

Active Publication Date: 2009-06-24
KONINKLIJKE PHILIPS ELECTRONICS NV
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  • Abstract
  • Description
  • Claims
  • Application Information

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However, with this known device it is not possible to carry o

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  • Microelectronic sensor device for DNA detection
  • Microelectronic sensor device for DNA detection
  • Microelectronic sensor device for DNA detection

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Embodiment Construction

[0055] Polymerase chain reaction (PCR) is a common technique used to amplify specific genetic elements from complex mixtures of DNA fragments. For example, it can be used for gene expression profiling, genetic analysis (eg SNP profiling), forensic analysis, or detection and identification of infectious agents. For this purpose, simultaneous analysis of a large number of DNA templates will be necessary. However, performing and analyzing multiple PCRs is a laborious and costly task.

[0056] It is therefore desirable to develop a reliable system that allows simultaneous performance and / or analysis of multiple PCRs. In order to use PCR for the identification of eg infectious agents such as microorganisms in body fluids or food, certain kinds of sequences must be analyzed. One approach to this is to amplify the DNA fragments with primers specific for each species that may be present. This is however a very laborious (and costly) protocol as each PCR must be run separately (the ...

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Abstract

The invention relates to a microelectronic sensor device and a method for the investigation of biological target substances (20), for example oligonucleotides like DNA fragments. In one embodiment, the device comprises a reaction surface (RS) to which target specific reactants (10) are attached and which lies between a sample chamber (SC) and an array of selectively controllable heating elements (HE). The temperature profile in the sample chamber (SC) can be controlled as desired to provide for example conditions for a PCR and/or for a controlled melting of hybridizations. The reactant (10) and/or the target substance (20) comprises a label (12) with an observable property, like fluorescence, that changes if the target substance (20) is bound to the reactant (10), said property being detected by an array of sensor elements, for example photosensors (SE). The fluorescence of the label (12) may preferably be transferred by FRET to a different fluorescent label (22) or quenched if the target substance (20) is bound.

Description

technical field [0001] The present invention relates to a microelectronic sensor device for the investigation of at least one biological target substance, in particular a nucleic acid molecule or a fragment thereof, comprising the use of target-specific reactants and sensor components. The invention also includes a method for the investigation of at least one biological target substance and the use of the microelectronic sensor device. Background technique [0002] US 2005 / 0009070A1 discloses a small biosensor comprising a multi-well plate in which the temperature in the reaction wells of the plate can be selectively controlled to provide optimal good condition. In US 6 864 140 B2 a microsensor is described having a localized heating element in the form of a thin film transistor formed on polysilicon on a substrate adjacent to the sample chamber where the (bio)chemical reaction takes place. However, further investigations of the sample in the sample chamber are not possibl...

Claims

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Application Information

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IPC IPC(8): C12Q1/68B01L3/00
CPCG01N21/6454B01L2300/1822G01N21/6428B01L2300/0636B01L2300/0819C12Q1/6825B01L3/5027G01N2021/6439B01L3/50851B01L2200/147B01L7/525B01L2300/1827B01L7/54G01N21/0332C12Q2565/1015C12Q2527/101C12Q2527/107
Inventor E·R·福森纳尔M·W·G·蓬吉H·R·施塔白特M·T·约翰逊
Owner KONINKLIJKE PHILIPS ELECTRONICS NV
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