16results about "Apparatus with spatial temperature gradients" patented technology

A centrifugal microfluidic technique for heat treating emulsion-divided independent reaction volumes (IRVs) within a centrifugal microfluidic chip, and displacing the emulsion into a monolayer presentation chamber (pc) for imaging. A deep treatment chamber (tc) is provided for the heat treatment, a nozzle having a hydrodynamic radius for forming the IRVs is provided for injecting a sample for the IRVs into the tc filled with a dense immiscible medium. The tc is adjacent a heat controlled element for collectively heat treating the IRVs within the tc, where the IRVs form a 3d packing arrangement. The tc is coupled to a presentation chamber (pc) by an opening through which the IRVs can be selectively displaced without collapsing. The pc is adjacent a window transparent to a wavelength for inspecting the pc.

A method for deparaffinizing formalin-fixed paraffin-embedded tissue (FFPE) in a centrifugal microfluidic biochip 10 with a fluidic system including a plurality of chambers 20, the method comprising the steps of: a. placing the formalin-fixed paraffin-embedded tissue (FFPE) in a first chamber 20 of the fluidic system of the centrifugal microfluidic biochip 10; b. introducing a fluid 30 into the first chamber 20; c. melting the paraffin 40 by adjusting the temperature of the first chamber 20 to a temperature above the melting temperature of the paraffin 40; d. separating the molten paraffin 40 by rotating the centrifugal microfluidic biochip 10 around a rotation axis 50; e. solidifying the separated liquid paraffin 40 by adjusting at least a sub-region of the first chamber 20 to a temperature below the melting temperature of the paraffin 40; and f. discharging a liquid phase containing the tissue from which the paraffin 40 has been removed from the first chamber 20.

The present subject matter relates generally to performing polymerase chain reactions (PCRs) in microfluidic devices, and more particularly to microfluidic systems, devices, and methods for performing a rapid polymerase chain reaction (PCR) protocol. In some embodiments, the presently disclosed subject matter provides a microfluidic system comprising a microfluidic instrument housing a microfluidic cartridge (or device) and any support component. In addition, the microfluidic cartridge may be, for example, any fluidic device or cartridge, a microfluidic device or cartridge, a DMF device or cartridge, a droplet actuator, a flow cell device or cartridge, etc.

A system for conducting one or more chemical, biochemical or biological reactions in a disposable comprising one or more wells at one or more set point reaction temperatures, comprising: - at least one disposable comprising the one or more wells in a body, wherein the wells are capable of acting as vessel for the one or more chemical, biochemical or biological reactions requiring one or more prescribed reaction temperatures according to a prescribed protocol, said body comprising a flat bottom side building a first heating surface capable of homogeneously conducting heat into the wells and a flat upper side comprising well openings, optionally sealed by means of a thin sealing foil, building a second heating surface capable of homogeneously conducting heat in the wells; - one or more gradient tempering unit GTUx, each comprising at least one tempering block comprising at least two temperable Peltier elements contacting a thermoconductive block, opposite to the Peltier elements a flat-surfaced area for positioning the at least one disposable on the thermoconductive block, said tempering block being configured to generate a set linear temperature profile in the wells of the disposable(s) when contacting the thermoconductive block; - a control unit comprising one or more processors configured to control the gradient tempering unit(s) GTUx for the implementation of the prescribed protocol for the one or more chemical, biochemical, or biological reactions; wherein the gradient tempering block with set linear temperature profile comprises at least one Peltier element temperable at a first temperature T1 and at least one Peltier element temperable at a second temperature T2, wherein T1 is higher than T2, and both Peltier elements are contacting the thermoconductive block outside of the positioning area for the disposable so that the linear temperature profile can be generated in the thermoconductive block between the two elements and conducted to the disposable while contacting the thermoconductive block. In particular, the solution can be used for cellular thermal shift assays (CETSA®), purified protein thermal shift assays (TSA) or any other protein denaturation or aggregation assays. The solution allows high throughput reactions or assays.

a disposable including a well; one or more gradient tempering units, each comprising a tempering block including a plurality of temperable Peltier elements contacting a thermally conductive block opposite a Peltier element, the gradient tempering units having a flat surface area for positioning the disposable on the thermally conductive block, the tempering blocks generating a set linear temperature profile for the well upon contacting the thermally conductive block; and a control unit for controlling the gradient tempering units; wherein the gradient tempering block includes a Peltier element capable of tempering at a temperature T1 and a Peltier element capable of tempering at a temperature T2, T1 being higher than T2, and both elements contacting the thermally conductive block outside of the positioning area for the disposable such that a linear temperature profile can be generated in the thermally conductive block between the two elements and conducted to the disposable through contact with the thermally conductive block.

The subject matter relates generally to performing polymerase chain reaction (PCR) in microfluidics devices and more particularly to a microfluidics system, device, and methods for performing rapid polymerase chain reaction (PCR) protocols. In some embodiments, the presently disclosed subject matter provides a microfluidics system including a microfluidics instrument housing a microfluidics cartridge (or device) along with any supporting components. Further, the microfluidics cartridge may be, for example, any fluidics device or cartridge, microfluidics device or cartridge, DMF device or cartridge, droplet actuator, flow cell device or cartridge, and the like.

Laboratory equipment (1) for incubating liquid laboratory samples contained in sample containers (130), in particular an incubator or incubation shaker, comprising: • a chamber (2) having at least one chamber wall (38) and a chamber opening (2a) for placing and removing the sample containers (130) into an interior (3) of the chamber, wherein the chamber opening can be closed by a door (120), • a heating device (190) for heating the chamber and / or the door, wherein the heating device has at least one heating coil arranged on the outside of at least one chamber wall (38) and / or a door wall (121) of the door (120), characterized in that • the at least one chamber wall (38) and / or the door wall (121) has at least one first surface area in which the heating power emitted by the at least one heating coil is greater than in a second surface area.

This disclosure describes various microfluidic devices that may be used in thermal cyclic fluid samples. Some of these devices may include a plurality of microwells that may be coupled by interconnected fluidic channels. These microwells may not be physically separated and yet may include features allowing for effective isolation of target molecules within each microwell. Other devices may include a plurality of microwells that may not be interconnected. The devices may also include mechanisms for causing a fluid to flow across the device. The devices may also include light-absorbing films for converting light energy to heat so as to allow for thermal cycling of samples within the microwells.

The subject matter relates generally to performing polymerase chain reaction (PCR) in microfluidics devices and more particularly to a microfluidics system, device, and methods for performing rapid polymerase chain reaction (PCR) protocols. In some embodiments, the presently disclosed subject matter provides a microfluidics system including a microfluidics instrument housing a microfluidics cartridge (or device) along with any supporting components. Further, the microfluidics cartridge may be, for example, any fluidics device or cartridge, microfluidics device or cartridge, DMF device or cartridge, droplet actuator, flow cell device or cartridge, and the like.

A fluid thermal processing device may include a substrate, a platform projecting from the substrate, a fluid heating element supported by the platform, a temperature sensing element, distinct from the fluid heating element, supported by the platform and an enclosure supported by and cooperating with the substrate to form a fluid chamber about the platform. The fluid chamber forms a volume of uniform thickness conforming to and about the platform.

The invention discloses a wide-temperature-range extremely-low-temperature storage test device which comprises a high-low-temperature storage box, a temperature monitoring control system and a heating / cooling system. The high-low temperature storage box is used for storing samples; the temperature monitoring control system is used for monitoring the temperature of the high-low temperature storage box in real time and controlling the heating / cooling system to heat or cool the high-low temperature storage box; the heating / cooling system is used for heating or cooling the high-low temperature storage box; the heating temperature is room temperature to 200 DEG C, and the cooling temperature is room temperature to-180 DEG C; the multi-temperature storage can be realized, the utilization rate is improved, the cost is reduced, and the application is wider.

The invention relates to a device for nucleic acid amplification, comprising: a channel; a temperature regulation system; a droplet manipulation mechanism configured to flow a droplet along the channel; wherein: the channel comprises portions in contact with heating zones of the temperature regulation system alternating with portions in contact with cooling zones of the temperature regulation system, the heating zones being configured to be at a higher temperature than the cooling zones, and wherein: each heating zone comprises an upstream section and a downstream section relative to the direction of flow in the channel, the downstream section is configured to be at a uniform temperature TH, the upstream section is configured to be at a temperature which is higher than TH; and / or: each cooling zone comprises an upstream section and a downstream section relative to the direction of flow in the channel, the downstream section is configured to be at a uniform temperature Tc, the upstream section is configured to be at a temperature which is lower than Tc.

A microfluidic chip includes multiple microfluidic channels each in fluid communication with a corresponding inlet. The inlets have a first spacing along a first linear path, wherein a central region of the microfluidic channels has a second spacing along a second linear path. The first spacing is greater than the second spacing. A microfluidic chip cassette is also disclosed.

To provide a microfluidic chip for presenting reaction volumes (IRVs) in discretization, processing, and a monolayer without collapsing the IRVs.SOLUTION: A deep treatment chamber (tc) is provided for heat treating emulsion-divided independent reaction volumes (IRVs) within a centrifugal microfluidic chip, and a nozzle having a hydrodynamic radius for forming the IRVs is provided for injecting a sample for the IRVs into the tc filled with a dense immiscible medium. The tc is adjacent to a heat controlled element for collectively heat treating the IRVs within the tc, where the IRVs form a 3d packing arrangement. The tc is coupled to a presentation chamber (pc) by an opening through which the IRVs can be selectively displaced without collapsing. The pc is adjacent to a window transparent to a wavelength for inspecting the pc.SELECTED DRAWING: None

The present invention relates to a sample carrier (1) for use in a rotation-based method for replicating or detecting DNA, comprising a disk-shaped substrate (2) and a number of cavities (4, 6, 8) formed in the substrate (2) for receiving a sample liquid, at least potentially containing DNA, during intended method steps (S1, ..., S4). The disk surface of the substrate (2) forms a heat input side (40), and the flat surface opposite the disk surface forms a heat output side. One cavity (6) or one of the cavities (4, 6, 8) is formed by an annular channel having first and second channel portions (50, 52) fluidly connected at both longitudinal ends by a connecting portion (54). The first channel portion (50) is offset in the thickness direction of the substrate (2) relative to the second channel portion (52).

The invention relates to a device for nucleic acid amplification, comprising: a channel; a temperature regulation system; a droplet manipulation mechanism configured to flow a droplet along the channel; wherein: the channel comprises portions in contact with heating zones of the temperature regulation system alternating with portions in contact with cooling zones of the temperature regulation system, the heating zones being configured to be at a higher temperature than the cooling zones, and wherein: each heating zone comprises an upstream section and a downstream section relative to the direction of flow in the channel, the downstream section is configured to be at a uniform temperature Th, the upstream section is configured to be at a temperature which is higher than Th; and / or: each cooling zone comprises an upstream section and a downstream section relative to the direction of flow in the channel, the downstream section is configured to be at a uniform temperature Tc, the upstream section is configured to be at a temperature which is lower than Tc.