9results about "Apparatus with spatial temperature gradients" patented technology

A centrifugal microfluidic technique for heat treating emulsion-divided independent reaction volumes (IRVs) within a centrifugal microfluidic chip, and displacing the emulsion into a monolayer presentation chamber (pc) for imaging. A deep treatment chamber (tc) is provided for the heat treatment, a nozzle having a hydrodynamic radius for forming the IRVs is provided for injecting a sample for the IRVs into the tc filled with a dense immiscible medium. The tc is adjacent a heat controlled element for collectively heat treating the IRVs within the tc, where the IRVs form a 3d packing arrangement. The tc is coupled to a presentation chamber (pc) by an opening through which the IRVs can be selectively displaced without collapsing. The pc is adjacent a window transparent to a wavelength for inspecting the pc.

Laboratory equipment (1) for incubating liquid laboratory samples contained in sample containers (130), in particular an incubator or incubation shaker, comprising: • a chamber (2) having at least one chamber wall (38) and a chamber opening (2a) for placing and removing the sample containers (130) into an interior (3) of the chamber, wherein the chamber opening can be closed by a door (120), • a heating device (190) for heating the chamber and / or the door, wherein the heating device has at least one heating coil arranged on the outside of at least one chamber wall (38) and / or a door wall (121) of the door (120), characterized in that • the at least one chamber wall (38) and / or the door wall (121) has at least one first surface area in which the heating power emitted by the at least one heating coil is greater than in a second surface area.

This disclosure describes various microfluidic devices that may be used in thermal cyclic fluid samples. Some of these devices may include a plurality of microwells that may be coupled by interconnected fluidic channels. These microwells may not be physically separated and yet may include features allowing for effective isolation of target molecules within each microwell. Other devices may include a plurality of microwells that may not be interconnected. The devices may also include mechanisms for causing a fluid to flow across the device. The devices may also include light-absorbing films for converting light energy to heat so as to allow for thermal cycling of samples within the microwells.

A fluid thermal processing device may include a substrate, a platform projecting from the substrate, a fluid heating element supported by the platform, a temperature sensing element, distinct from the fluid heating element, supported by the platform and an enclosure supported by and cooperating with the substrate to form a fluid chamber about the platform. The fluid chamber forms a volume of uniform thickness conforming to and about the platform.

The invention discloses a wide-temperature-range extremely-low-temperature storage test device which comprises a high-low-temperature storage box, a temperature monitoring control system and a heating / cooling system. The high-low temperature storage box is used for storing samples; the temperature monitoring control system is used for monitoring the temperature of the high-low temperature storage box in real time and controlling the heating / cooling system to heat or cool the high-low temperature storage box; the heating / cooling system is used for heating or cooling the high-low temperature storage box; the heating temperature is room temperature to 200 DEG C, and the cooling temperature is room temperature to-180 DEG C; the multi-temperature storage can be realized, the utilization rate is improved, the cost is reduced, and the application is wider.

The invention relates to a device for nucleic acid amplification, comprising: a channel; a temperature regulation system; a droplet manipulation mechanism configured to flow a droplet along the channel; wherein: the channel comprises portions in contact with heating zones of the temperature regulation system alternating with portions in contact with cooling zones of the temperature regulation system, the heating zones being configured to be at a higher temperature than the cooling zones, and wherein: each heating zone comprises an upstream section and a downstream section relative to the direction of flow in the channel, the downstream section is configured to be at a uniform temperature TH, the upstream section is configured to be at a temperature which is higher than TH; and / or: each cooling zone comprises an upstream section and a downstream section relative to the direction of flow in the channel, the downstream section is configured to be at a uniform temperature Tc, the upstream section is configured to be at a temperature which is lower than Tc.

A microfluidic chip includes multiple microfluidic channels each in fluid communication with a corresponding inlet. The inlets have a first spacing along a first linear path, wherein a central region of the microfluidic channels has a second spacing along a second linear path. The first spacing is greater than the second spacing. A microfluidic chip cassette is also disclosed.

To provide a microfluidic chip for presenting reaction volumes (IRVs) in discretization, processing, and a monolayer without collapsing the IRVs.SOLUTION: A deep treatment chamber (tc) is provided for heat treating emulsion-divided independent reaction volumes (IRVs) within a centrifugal microfluidic chip, and a nozzle having a hydrodynamic radius for forming the IRVs is provided for injecting a sample for the IRVs into the tc filled with a dense immiscible medium. The tc is adjacent to a heat controlled element for collectively heat treating the IRVs within the tc, where the IRVs form a 3d packing arrangement. The tc is coupled to a presentation chamber (pc) by an opening through which the IRVs can be selectively displaced without collapsing. The pc is adjacent to a window transparent to a wavelength for inspecting the pc.SELECTED DRAWING: None

The invention relates to a device for nucleic acid amplification, comprising: a channel; a temperature regulation system; a droplet manipulation mechanism configured to flow a droplet along the channel; wherein: the channel comprises portions in contact with heating zones of the temperature regulation system alternating with portions in contact with cooling zones of the temperature regulation system, the heating zones being configured to be at a higher temperature than the cooling zones, and wherein: each heating zone comprises an upstream section and a downstream section relative to the direction of flow in the channel, the downstream section is configured to be at a uniform temperature Th, the upstream section is configured to be at a temperature which is higher than Th; and / or: each cooling zone comprises an upstream section and a downstream section relative to the direction of flow in the channel, the downstream section is configured to be at a uniform temperature Tc, the upstream section is configured to be at a temperature which is lower than Tc.