Production method of silver-clad gold nano-rods and their application

A technology of gold nanorods and nanorods, which is applied in nanostructure manufacturing, nanotechnology, nanotechnology, etc., can solve the problems of expensive instruments, cumbersome operations, high false positive and false negative rates of PCR, etc., and achieve the aspect ratio distribution Uniform, wide linear range and highly reproducible results

Active Publication Date: 2015-04-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, traditional methods for detecting bacteria are done by morphological characterization or biochemical analysis, which cannot provide accurate conclusions on bacterial species so that identification at the strain level and detection of bacteria isolated from the environment cannot be achieved; while testing It is very time-consuming to carry out and data processing, and cannot meet the fast and accurate real-time monitoring required for bacterial detection
The PCR method is greatly affected by the sample, especially in the inspection of food-borne harmful microorganisms, the ingredients in the food (sugars, acids, oils, etc.) will interfere with the normal progress of the reac...

Method used

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  • Production method of silver-clad gold nano-rods and their application
  • Production method of silver-clad gold nano-rods and their application
  • Production method of silver-clad gold nano-rods and their application

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Synthesis of Example 1 AuAg nanorods

[0041] First, weigh 0.364g CTAB, fully dissolve it in 20mL water, then add 10mL gold nanorod solution to the CTAB aqueous solution under stirring at 27°C, keep stirring for 2min, then add Add 3mM AgNO 3 , 650 μL of 0.1M VC, 1200 μL of 0.1M NaOH, stirring continuously for 5-10 min and standing overnight, the reaction temperature is 27°C.

[0042] Note: Depending on the size of the synthesized AuAg nanorods, choose AgNO 3 The volume of the solution also varies, i.e. the choice of AgNO 3 The volumes of the solutions were 1250 μL, 2500 μL and 3750 μL, respectively.

[0043] figure 1 For Au nanorods and AuAg nanorods TEM (transmission electron microscope image). The present invention has carried out silver-coating experiments on Au nanorods of different lengths (Figures A, B, C), and obtained AuAg nanorods (D, E, F) of different lengths. According to the scale below the figure, through the silver-coating experiments, AuAg nano The...

Embodiment 2

[0044] Example 2 AuAg nanorods used in eaeA gene detection

[0045] Schematic diagram of detection of Escherichia coli O157:H7eaeA gene by AuAg nanorods figure 2 shown. The oligonucleotide probes were modified on the AuAg nanorods, and the fluorescent signal was enhanced after the oligonucleotide probes were hybridized with some nucleotides connected with the fluorescent dye cy3. When the target DNA appears, the probe is combined with the target DNA, and the fluorescence is weakened at this time, so it is applied to the detection of the E. coli O157:H7eaeA gene according to the principle of fluorescence enhancement-decrease.

[0046] The specific method is as follows:

[0047] (1) AuAg nanorod surface modification MUDA

[0048] First, the synthesized AuAg nanorods with a length of 50 nm were centrifuged at 8500 rpm at 25°C, and then, according to the principle of ligand exchange reaction, the surface was modified with mercaptoundecanoic acid (MUDA), and 3 mL of purified Au...

Embodiment 3

[0060] Performance Analysis of Example 3 AuAg Nanorods

[0061] (1) Stability

[0062] Different batches of AuAg nanorods prepared by the present invention were used to detect the eaeA gene of Escherichia coli O157:H7. The results are shown in Table 1. The result shows that the gene recovery rate is 98.36-101.67%, and the error is small, indicating that the AuAg nanorod and the detection method of the present invention have good stability and repeatability.

[0063] Table 1 Detection of Escherichia coli O157:H7eaeA gene recovery rate and error results

[0064]

[0065] Note: This experiment is a recovery experiment, the background concentration is consistent, and the variable is the spiked concentration.

[0066] (2) specificity

[0067] Using the AuAg nanorods prepared in the present invention to detect different gene sequences, including the target sequence completely complementary to the oligonucleotide probe part (5'-CGTCGCGGTATAAGTAATGGTATCGGCGTT-3', that is, as sh...

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Abstract

The invention discloses a production method of silver-clad gold nano-rods and their application and belongs to the technical field of food quality detection and analysis. Au@ag nano-rods are produced, and with the surface enhanced fluorescence of the nano-rods, the nano-rods are successfully applied to the detection of the gene O157:H7easeA of E. coli. Compared with the traditional methods, the method has the advantages that detection limit is greatly lowered, sensitivity is improved, and good stability is achieved. According to the principle of fluorescence enhancement-weakening, a standard curve for gene O157:H7ease detection of E. coli is built, a linear range is from 10<-17> M to 10<-11> M, correlation coefficient R2 is 0.9947, and the detection limit is 3.33*10<-18> M (S/N = 3). The method has the advantages that specificity is good, labelling experiments show that eaeA gene recovery rate is 98.36% to 101.67%, with good stability, and the nano-rods are applicable to the detection of the gene O157:H7ease of E. coli and have a promising application prospect.

Description

technical field [0001] The invention relates to a preparation method and application of silver-coated gold nanorods, belonging to the technical field of food quality detection and analysis. Background technique [0002] Escherichia coli (E.coli) is a representative bacterium of the genus Escherichia (Escherichia). It is the most important and most abundant bacterium in the intestinal tract of humans and many animals, and it mainly parasitizes in the large intestine. When it invades some parts of the human body, it can cause infections such as peritonitis, cholecystitis, cystitis and diarrhea. Symptoms of people infected with E. coli are stomach pain, vomiting, diarrhea and fever. Infection can be fatal, especially in children and the elderly. [0003] The pathogenic substances of Escherichia coli are mainly: (1) invasiveness: including K antigen and settlement factor; (2) endotoxin: the cell wall of Escherichia coli containing endotoxin has endotoxin activity, and the lipi...

Claims

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Application Information

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IPC IPC(8): B82B3/00B82Y30/00B82Y40/00G01N33/569G01N33/531
Inventor 孙秀兰孙嘉笛张银志
Owner JIANGNAN UNIV
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