Loop-mediated isothermal amplification detection method for detecting Fusarium oxysporum of Chinese wolfberry root rot, and detection primers and verification method thereof

Abstract

The invention discloses a loop-mediated isothermal amplification detection method for detecting Fusarium oxysporum of Chinese wolfberry root rot, and detection primers and a verification method thereof. The detection primers are designed by adopting LAMP (Loop-mediated Isothermal Amplification) design software Primer Explorer V4, and comprise two outer primers F3 and B3 and two inner primers FIP and BIP. The invention has the following beneficial effect: By the detection method, the primers and the verification method, five Fusarium oxysporum from different geographical sources is yellow green, namely positive after LAMP detection, but the control and other pathogenic bacteria are orange, namely negative. The invention has the advantages of strong primer specificity, high detection sensitivity, high reaction speed, direct observation of results by naked eyes, high accuracy, difficulty in pollution, direct detection of suspected disease samples and the like. And meanwhile, comparison and judgment are performed through color development, so that the detection accuracy is greatly improved.

Description

Technical field [0001] The invention relates to the technical field of detection of pathogens of plant diseases, in particular to a loop-mediated isothermal amplification detection method for detecting Fusarium oxysporum of Lycium barbarum root rot, and its detection primers and verification methods. Background technique [0002] Symptoms of Lycium barbarum root rot disease: At the initial stage of the disease, the leaves of the diseased plant are gray-green, wilting at noon, and can recover sooner or later. After that, it did not recover, and the leaves turned yellow and died slowly. The rhizome is slightly swollen, the phloem becomes reddish brown and soft, with white hyphae on the outside, and reddish-brown fine streaks on the xylem, sometimes infiltrating into the deep layer. The phloem of the tap root and lateral roots became soft and brown and died. The epidermis is longitudinally cracked, and there are many small white powdery spots in the phloem, which are easy to peel ...

AI Technical Summary

Problems solved by technology

Although the above culture method can detect Fusarium oxysporum, it requires staff to observe the shape change in real time and record it before making a judgment

The above method has a long cultivation cycle and different judgment standards

In particular, rapid field tests cannot be applied, and the cultivation period is too long. After the test results are confirmed, large-scale disasters have occurred in the field, and it is impossible to prevent and control them in advance.

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