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Thyroid hormone analysis by mass spectrometry

a mass spectrometry and thyroid hormone technology, applied in the field of hormone analysis and mass spectrometry, can solve the problem of relatively low detection limit, and achieve the effect of fast and accurate method of hormone analysis

Active Publication Date: 2013-07-30
GEORGETOWN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The invention provides a way to quickly and accurately analyze hormones using a mass spectrometer. This can be done with samples of saliva or urine, which are easier to collect and send to a clinic for analysis. The method is fast, simple, and accurate, and can simultaneously or sequentially analyze multiple hormones. The system includes reagents for preparing the sample and performing the analysis on the mass spectrometer.

Problems solved by technology

In addition, the limit of detection can be fairly low.

Method used

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  • Thyroid hormone analysis by mass spectrometry
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  • Thyroid hormone analysis by mass spectrometry

Examples

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examples

[0091]The invention will now be demonstrated using the following examples, provided to demonstrate but not limit the embodiments of the present invention:

[0092]1. Analysis of a Sample for Thyroid Hormones

[0093]A sample of 100 μL of plasma was used. Proteins were precipitated with 150 μL of acetonitrile, capped and vortexed. The sample was then centrifuged, and 200 μL of the supernatant was injected onto a Supelco LC-18-DB™ chromatographic column equipped with Supelco Discovery C18™ guard column, coupled to a tandem mass spectrometer (LC / MS / MS). The column was washed with 20% methanol in 5 mM ammonium acetate for 3 minutes. The valve was switched and the sample was eluted in 75% to 95% methanol. The total run time was 6 minutes. Slight adjustments to the volumes, concentrations and times described can be made, as is known to those skilled in the art.

[0094]The eluant was introduced into an ion-spray ionization chamber and analyzed by API 2000™ mass spectrometer using the negative mode...

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Abstract

Methods, systems and kits for the simultaneous or sequential analysis of one or more hormones by mass spectrometry are disclosed. The methods require minimal sample size and minimal preparation time. The methods include ionizing the hormones and analyzing the hormones by mass spectrometry. In addition, methods, systems and kits for the simultaneous or sequential analysis of thyroid hormones are disclosed including ionization of the thyroid hormones in the negative mode using an electrospray source.

Description

CONTINUING DATA[0001]This is an application that is a Reissue of U.S. application Ser. No. 10 / 823,690 filed Apr. 14, 2004 which is now U.S. Pat. No. 7,618,827, which claims benefit of U.S. Provisional Patent Application No. 60 / 462,745, filed Apr. 14, 2003, each of which is incorporated herein by reference in its entirety.FIELD OF THE INVENTION[0002]The present invention combines the fields of hormone analysis and mass spectrometry. In particular the invention relates to analyzing thyroid hormones using mass spectrometry.BACKGROUND OF THE INVENTION[0003]Hormones are biological messengers. They are synthesized by specific tissues (glands) and are secreted into the blood. The blood carries them to target cells where they act to alter the activities of the target cells.[0004]Hormones are chemically diverse, and are generally categorized into three main groups: (1) small molecules derived from amino acids, for example thyroxine, (2) polypeptides or proteins, for example insulin and thyro...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): G01N33/53G01N33/00C12N9/10G01N33/68G01N33/78
CPCG01N33/6848G01N33/78Y10T436/24
Inventor SOLDIN, STEVEN J.
Owner GEORGETOWN UNIV
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