41 results about "A deoxyribonuclease" patented technology

The invention discloses an extraction method and application of high-molecular-weight turbellarian worm genome DNA, and belongs to the field of turbellarian worm genome extraction.The turbellarian worm genome DNA extraction is achieved by improving a lysate formula and removing ethylenediaminetetraacetic acid and increasing Mg < 2 + > on the basis of a conventional reagent in a classic Tris-HCl-phenol-chloroform method to inhibit activity of deoxyribonuclease II in a turbellarian worm body to inhibit degradation of genome DNA, and the high-molecular-weight turbellarian worm genome DNA is obtained. Meanwhile, ribonuclease A is added, RNA in cells can be degraded in the tissue cell digestion process, so that the step of two times of purification is omitted, the loss of DNA yield is reduced, experimental steps are simplified, especially the step of DNA purification is reduced, the problem of degradation in the extraction process of the turbellarian turbellarian worm genome DNA is solved, and the genome DNA yield (gt; 10 micrograms per turbellarian worm), and the turbellarian worm genome ultra-long sequencing or PacBio high-fidelity sequencing requirements are met.

The present application relates to a deoxyribonuclease mutant and its application in preparing PDRN. The amino acid sequence of the deoxyribonuclease mutant is shown as SEQ ID NO. 1, and the nucleotide sequence of the encoding gene is shown as SEQ ID NO. 2. The present application also provides the application of the deoxyribonuclease mutant and / or the encoding gene of the deoxyribonuclease mutant in preparing PDRN, and the specific application method. The present application is based on the deoxyribonuclease expressed by the DNASE1 gene of bovine pancreas tissue, and the amino acids at positions 69, 132 and 215 are selected for iterative saturation mutation. Compared with the wild-type enzyme, the enzyme activity of the deoxyribonuclease mutant A69H-S132R-Q215C is increased by 2.15 times, reaching 4.1x10 6 U / mL, and the DNA extraction rate of salmon PDRN prepared by using the mutant enzyme prepared by the present application is 15.2%, the DNA content is 99.1%, and the molecular weight is 1.05 million Daltons, which is significantly improved compared with the wild-type enzyme, and the PDRN in this molecular weight range has more significant biological activity.

The application provides a kit for extracting RNA and application thereof. In the application, deoxyribonuclease (DNase) is used for DNA digestion in the process of magnetic bead extraction. The reaction environment can meet the optimal reaction environment requirement of the deoxyribonuclease (DNase), and at the same time, a hydrophobic environment is provided when the magnetic beads are adsorbed, so as to ensure the yield of nucleic acid. In addition, an emulsion of an oil is added to increase the total volume during the extraction operation, so as to facilitate the convenience of automatic extraction operation of the instrument. The extracted RNA has high yield and high purity, and the method can easily realize automatic extraction of the instrument. The requirement of extracting high-purity RNA quickly and conveniently in the process of clinical practical application is solved, the difficulty and cost of extracting high-purity RNA are greatly reduced, and the extracted RNA can be well adapted to subsequent RT-PCR and RNA-seq application.

Abstract Described herein, in one aspect, are antigen presenting cells (APCs) comprising an exogenous nucleic acid encoding one or more candidate antigens, wherein the one or more candidate antigens are expressed and presented with MHC class I or MC class II molecules; a molecular reporter of Granzyme B (GzB) activity; and c) an exogenous inhibitor of caspase-activated deoxyribonuclease (CAD)-mediated DNA degradation, a CAD knockout, or a caspase knockout (e.g., caspase 3 knockout). Described herein, in another aspect, is a system for detection of recognized antigen presentation by an antigen presenting cell to a cytotoxic lymphocyte or NK cell. Abstract 2018 / 22761 oM - cell Target ml Target cell cell cell Target Target Target SUBSTITUTE SHEET (RULE 26) cell cell cell Target Target cell cell 1 / 28 my Isolate recognized cell Library of target cells target cells and displaying different Add T cells from sample sequence antigens antigens of interest. CTLs deliver cytotoxic granules to target cells displaying cognate antigen FIG. 1 PCT / US2018 / 036663 20 26 20 53 56 07 J ul 2 02 6 2 0 2 6 2 0 5 3 5 6 0 7 J u l 2 0 2 6 2 0 1 8 / 2 2 7 6 1 o M a n d m y 1 / 2 8 m y L i b r a r y o f t a r g e t c e l l s d i s p l a y i n g d i f f e r e n tA d d T c e l l s f r o m s a m p l e of interest. CTLs deliver c y t o t o x i c g r a n u l e s t o t a r g e t c e l l s d i s p l a y i n g c o g n a t e a n t i g e n P C T / U S 2 0 1 8 / 0 3 6 6 6 3

Some embodiments of the present disclosure relate to one or more compositions that upregulate the production of one or more sequences of mRNA. The sequences of mRNA may encode for translation of a target biomolecule, thereby causing an increase in bioavailability of the target biomolecule within a subject that is administered the one or more compositions. In some embodiments of the present disclosure, the target biomolecule is a fusion protein with an Fc fragment, such as a toll-like receptor 3-Fc (TLR3-Fc). In some embodiments of the present disclosure, the target biomolecule is toll-like receptor 9-Fc (TLR9-Fc). In some embodiments of the present disclosure, the target biomolecule is deoxyribonuclease I-Fc (DNAse I-Fc). In some embodiments of the present disclosure, the target biomolecule is neural growth factor-Fc (NGF-Fc). In some embodiments of the present disclosure, the target biomolecule is insulin-Fc.

The present invention provides a method for reducing the amount of DNA remaining in a biopolymer so as to increase the purity of the biopolymer when producing the biopolymer by means of a microorganism. This biopolymer production method includes: an endodeoxyribonuclease treatment step for bringing an endodeoxyribonuclease into contact with biopolymer granules obtained by culturing a microorganism capable of producing a biopolymer; and a pH adjustment step for keeping the biopolymer granules under the condition of a pH of 7.0 or higher at least at one time point after the start of said endodeoxyribonuclease treatment.

Some embodiments of the present disclosure relate to one or more compositions that upregulate the production of one or more sequences of mRNA. The sequences of mRNA may encode for translation of a target biomolecule, thereby causing an increase in bioavailability of the target biomolecule within a subject that is administered the one or more compositions. In some embodiments of the present disclosure, the target biomolecule is a fusion protein with an Fc fragment, such as a toll-like receptor 3-Fc (TLR3-Fc). In some embodiments of the present disclosure, the target biomolecule is toll-like receptor 9-Fc (TLR9-Fc). In some embodiments of the present disclosure, the target biomolecule is deoxyribonuclease I-Fc (DNAse I-Fc). In some embodiments of the present disclosure, the target biomolecule is neural growth factor-Fc (NGF-Fc). In some embodiments of the present disclosure, the target biomolecule is insulin-Fc.

The present invention discloses a recombinant Vibrio natriegens and a method for promoting the secretion and expression of exogenous proteins. The recombinant Vibrio natriegens is constructed by using the Vibrio natriegens with the preservation number of CICC 10908 as the chassis cell, knocking out the deoxyribonuclease gene on its genome, and inserting a combined expression cassette of T7 RNA polymerase and aminoglycoside adenyltransferase. The recombinant plasmid carrying the exogenous protein is transformed into the above-mentioned recombinant Vibrio natriegens, and the seed liquid is inoculated into the fermentation medium. After culturing to the mid-logarithmic growth phase, IPTG and NaCl are added, and then the culture is continued to obtain a fermentation broth containing the exogenous protein. The present invention solves the problem of secreting and expressing exogenous proteins by Vibrio natriegens at the fermentation level for the first time, with a short culture time, low cost, and certain universality, providing technical guidance for the research of Vibrio natriegens engineering bacteria in the fields of protein engineering and fermentation engineering.

The present invention provides compositions that modulate gut microbiota and / or the activity of deoxyribonucleases (e.g., in the gastrointestinal tract) and are useful in the treatment of diseases and disorders related to the activity of deoxyribonucleases including, for example, neurodegenerative, inflammatory, and metabolic diseases and cancer.

The application discloses a kind of nanoparticles for removing lung mucus and its preparation method and application, it is related to the field of biological medicine.The nanoparticles of the application are composed of inner core and shell coated on the surface of the inner core, wherein the inner core contains citric acid and photoluminescent polymer-aliphatic polyester copolymer;The shell contains deoxyribonuclease and hydrophilic polymer.The nanoparticles provided by the application have excellent biocompatibility and multifunctional integration characteristics, which realizes effective penetration of lung mucus barrier, specific degradation of DNA network, down-regulation of pro-inflammatory factors and inhibition of mucus production in sequence through cascade response mechanism, breaking through the limitations of traditional single therapy.In addition, the in vivo experimental results show that it can significantly reduce the thickness of lung mucus and the protein concentration of lavage fluid, and effectively relieve airway obstruction, and can be used for removing lung mucus.

The invention provides a preparation method of decellularized omentum majus, and relates to the technical field of biomedical materials.The method comprises the steps that in a closed container, omentum majus subjected to freeze-drying treatment is placed in a mixed solvent to be subjected to degreasing treatment, and a first intermediate is obtained; performing vacuum pulse treatment on the closed container in the degreasing treatment process; cleaning the first intermediate to obtain a second intermediate; performing circulating oscillation treatment on the second intermediate for at least one time to obtain a third intermediate; the third intermediate is cleaned in a phosphate buffer solution and then placed in a compound enzyme solution containing pancreatin and deoxyribonuclease I to be subjected to oscillation treatment, and a fourth intermediate is obtained; the fourth intermediate is sequentially washed with deionized water and a phosphate buffer solution and then placed in a sucrose solution to be soaked, then freeze-drying and sterilization are sequentially conducted, and the decellularized omentum majus is obtained. By adopting the method disclosed by the invention, the decellularized omentum majus with high purity, low residue and complete structure can be obtained.

Some embodiments of the present disclosure relate to one or more compositions that upregulate the production of one or more sequences of mRNA. The sequences of mRNA may encode for translation of a target biomolecule, thereby causing an increase in bioavailability of the target biomolecule within a subject that is administered the one or more compositions. In some embodiments of the present disclosure, the target biomolecule is a fusion protein with an Fc fragment, such as a toll-like receptor 3-Fc (TLR3-Fc). In some embodiments of the present disclosure, the target biomolecule is toll-like receptor 9-Fc (TLR9-Fc). In some embodiments of the present disclosure, the target biomolecule is deoxyribonuclease I-Fc (DNAse I-Fc). In some embodiments of the present disclosure, the target biomolecule is neural growth factor-Fc (NGF-Fc). In some embodiments of the present disclosure, the target biomolecule is insulin-Fc.

The invention discloses lung glial cells as well as an in-vitro culture method and application thereof. The in-vitro culture method comprises the steps that in-vitro mouse lung tissue is subjected to mechanical separation and enzymolysis digestion, a single-cell suspension is prepared, enzymolysis digestion is conducted by adding a mixed digestive enzyme solution, and the mixed digestive enzyme solution comprises type I collagenase and deoxyribonuclease I; the single cells are placed in a DMEM complete medium for subculture, the pulmonary glial cells are obtained, and the DMEM complete medium contains the heparin-binding epidermal growth factors. According to the in-vitro culture method, the lung glial cells can be successfully obtained, and the lung glial cells are high in purity, good in cell morphology and state, normal in function and suitable for follow-up cell experiments.

The present invention provides compositions that modulate gut microbiota and / or the activity of deoxyribonucleases (e.g., in the gastrointestinal tract) and are useful in the treatment of diseases and disorders related to the activity of deoxyribonucleases including, for example, neurodegenerative, inflammatory, and metabolic diseases and cancer.

The present invention discloses a novel PDRN, a supramolecular liposome containing the PDRN, and a preparation method and use thereof. The preparation method of the novel PDRN comprises: (1) mixing tissue raw materials with PBS, crushing, enzymolysis, adding SDS, Tween20 solution, polyethylene glycol octylphenyl ether, TE buffer, NaCl, stirring, centrifuging, mixing the supernatant with anhydrous ethanol, and standing to obtain a crude DNA extract; (2) using an ultrasonic interrupter to interrupt and shear the tissue; (3) using restriction endonucleases and deoxyribonucleases to perform enzymolysis, etc. The PDRN supramolecular liposome is obtained by encapsulating the above-mentioned PDRN with fatty acids extracted from marine microalgae. The present invention uses fish testis as a raw material source, broadens the range of raw material selection, reduces preparation costs, and the organic reagent used in the preparation method of the present invention is ethanol, which is easy to remove and relatively harmless.

This invention belongs to the medical field and provides a calcium carbonate manganese nanoprobe for in situ imaging detection of vascular epidermal growth factor, its preparation method, and its application. In this strategy, a DNA strand (P1 and P2) containing a protein recognition antibody, a protein recognition sequence, and a deoxyribonuclease, along with a molecular beacon DNA strand (H1), is stirred in a DMEM cell culture medium containing calcium chloride and manganese chloride to prepare the calcium carbonate manganese nanoprobe. In acidic tumor cells, this nanoprobe releases P1, P2, H1, and manganese ions. P1 and P2 can simultaneously recognize proteins, forming a complete deoxyribonuclease center, which catalyzes the cleavage of H1 under the action of the cofactor manganese ions, generating a fluorescent signal. This strategy achieves specific in situ dynamic imaging of low-abundance proteins in vivo and has great potential in biomedical research.

Therapeutic compositions for treating a subject, the compositions including a polymeric nanoparticle, an active agent encapsulated within the polymeric nanoparticle, and a delivery vehicle for targeted delivery of the polymeric nanoparticle and encapsulated agent to a target tissue in the subject. The therapeutic compositions can be designed for targeting the destruction of a Nuclei neutrophil extracellular trap (NET) in a venous thrombus in a subject. The active agent can be an enzyme, including deoxyribonuclease 1 (DNase 1) enzyme. Methods of treating a subject using the therapeutic compositions are provided, including methods of treating venous thrombus, deep vein thrombosis, or a related blood clotting disease and / or condition.

Some embodiments of the present disclosure relate to one or more compositions that upregulate the production of one or more sequences of mRNA. The sequences of mRNA may encode for translation of a target biomolecule, thereby causing an increase in bioavailability of the target biomolecule within a subject that is administered the one or more compositions. In some embodiments of the present disclosure, the target biomolecule is a fusion protein with an Fc fragment, such as a toll-like receptor 3-Fc (TLR3-Fc). In some embodiments of the present disclosure, the target biomolecule is toll-like receptor 9-Fc (TLR9-Fc). In some embodiments of the present disclosure, the target biomolecule is deoxyribonuclease I-Fc (DNAse I-Fc). In some embodiments of the present disclosure, the target biomolecule is neural growth factor-Fc (NGF-Fc). In some embodiments of the present disclosure, the target biomolecule is insulin-Fc.

This invention provides a Csm6-based deoxyribonuclease sensor, characterized by comprising the following components: (1) a deoxyribonuclease enzyme chain and a deoxyribonuclease substrate chain, wherein the nucleotide sequence of the deoxyribonuclease substrate chain is shown in SEQ ID No. 1 or SEQ ID No. 2 of the sequence listing, and the nucleotide sequence of the deoxyribonuclease enzyme chain is shown in SEQ ID No. 10 of the sequence listing; (2) a Csm6 protease; and (3) a single-stranded RNA reporter probe modified with fluorescent and quenching groups, wherein the sequence of the single-stranded RNA reporter probe modified with fluorescent and quenching groups is shown in SEQ ID No. 16 of the sequence listing. This invention also provides an application of this deoxyribonuclease sensor for lead detection. The deoxyribonuclease sensor provided by this invention can improve the sensitivity and selectivity of existing biosensors in lead ion detection.

The invention discloses a ternary co-modified engineered cornu cervi pantotrichum MSCs exosome and a preparation method and application thereof, and belongs to the technical field of biological medicine, the ternary co-modified engineered cornu cervi pantotrichum MSCs exosome comprises a cornu cervi pantotrichum MSCs exosome, a poly-arginine polypeptide-deoxyribonuclease I compound located on the surface of the cornu cervi pantotrichum MSCs exosome, and an RGD polypeptide modified on the surface of the cornu cervi pantotrichum MSCs exosome and containing an Arg-Gly-Asp sequence. In order to solve the problem that an integrated treatment mode of multiple pathological links cannot be realized during existing RA treatment, three active components, namely poly-arginine polypeptide (P-Arg), deoxyribonuclease I (Dnase I) and RGD polypeptide, are cooperatively modified on the surface of an exosome of pilose antler MSCs for the first time to form a surface engineering modified exosome complex; multiple functions of immunoregulation, cfDNA removal, targeted delivery and tissue protection can be realized in RA focuses, and a brand-new and systematic treatment means is provided for rheumatoid arthritis.

The present application belongs to the technical field of genetic engineering, and particularly relates to a method for preparing recombinant deoxyribonuclease by prokaryotic expression. The method comprises the following steps: S1, connecting a target gene into a multiple cloning site of an expression vector to construct a recombinant expression vector, wherein the target gene comprises an X X A tag coding gene and a deoxyribonuclease coding gene; S2, introducing the recombinant expression vector into E. coli; S3, culturing the recombinant E. coli and inducing expression; and S4, collecting bacterial bodies after centrifugation of the recombinant E. coli culture solution, ultrasonic crushing, centrifugal collection of cell crushing supernatant, and then affinity purification to obtain a deoxyribonuclease fusion protein. The present application fuses the X X A tag with the deoxyribonuclease, adopts a prokaryotic expression system, has high host bacterium activity in the process of inducing expression, realizes high-efficiency expression of DNase, and significantly improves the soluble expression level, and has the advantages of good DNase biological activity and high stability.

The invention relates to the technical field of biology, and particularly discloses a salt-tolerant deoxyribonuclease mutant I and application thereof. On the basis of an amino acid sequence as shown in SEQ ID NO.1, aspartic acid at the 42nd site is mutated into arginine D42R, tyrosine at the 76th site is mutated into lysine Y76K, and the amino acid sequence of the mutant is as shown in SEQ ID NO.7; the invention relates to an application of a salt-tolerant deoxyribonuclease I mutant in removal of low-concentration residual DNA in RNA (Ribonucleic Acid). According to the salt-tolerant DNase I mutant disclosed by the invention, a complex salt ion concentration adjusting step which needs to be carried out due to salt ion inhibition is reduced, so that time and reagent cost are saved; meanwhile, due to the efficient degradation capacity on low-concentration DNA, subsequent experiment failures or unqualified products caused by DNA residues are avoided, and the overall experiment and production efficiency is improved.

Some embodiments of the present disclosure relate to one or more compositions that upregulate the production of one or more sequences of mRNA. The sequences of mRNA may encode for translation of a target biomolecule, thereby causing an increase in bioavailability of the target biomolecule within a subject that is administered the one or more compositions. In some embodiments of the present disclosure, the target biomolecule is a fusion protein with an Fc fragment, such as a toll-like receptor 3-Fc (TLR3-Fc). In some embodiments of the present disclosure, the target biomolecule is toll-like receptor 9-Fc (TLR9-Fc). In some embodiments of the present disclosure, the target biomolecule is deoxyribonuclease I-Fc (DNAse I-Fc). In some embodiments of the present disclosure, the target biomolecule is neural growth factor-Fc (NGF-Fc). In some embodiments of the present disclosure, the target biomolecule is insulin-Fc.

The present invention discloses a kind of decellularized meniscus of extracellular matrix high retention and preparation method thereof.The method includes the following steps:(1) biomaterial is subjected to freeze-thaw cycle;(2) the biomaterial after freeze-thaw cycle treatment in step (1) is taken out sequentially through cell elution liquid:A liquid, B liquid, C liquid, D liquid until biomaterial decellularization ends;Wherein when eluting with A liquid, B liquid, C liquid, D liquid, the biomaterial is fixed in container and carried out;Wherein, A liquid is Triton X 100 solution;B liquid, D liquid are PBS;C liquid is the nuclease solution of deoxyribonuclease I and ribonuclease A composition;Cell elution liquid does not contain trypsin;A liquid elution time is 7 days.The decellularization method of the present invention can retain meniscus extracellular matrix components as much as possible while completely removing cell components, provide original ecological microenvironment for cell growth.

The application provides a PDRN and an extraction method and application thereof, and belongs to the technical field of PDRN extraction. The extraction method of the PDRN comprises the following steps: subjecting plant tissues to stress treatment, so that the cells are in an irreversible damage state; subjecting the plant tissues subjected to the stress treatment to incubation treatment, so that DNA is selectively partially degraded under the action of endogenous deoxyribonuclease; and subjecting the plant tissues subjected to the incubation treatment to crushing treatment and extraction and purification treatment, to obtain the PDRN. The extraction method selectively degrades DNA under mild conditions by using endogenous enzymes (such as DNase) of plants, and has the advantages of high yield, high efficiency, low cost and green environmental protection; meanwhile, the PDRN extracted by the extraction method also has the advantages of high purity, high physiological activity and high safety.

The invention discloses a sensor for detecting cadmium, the sensor comprises a reference electrode, a counter electrode, a working electrode and an electrolyte, the working electrode comprises a substrate, the surface of the substrate is coated with nanogold modified graded porous carbon Au-coated HPC, the surface of the substrate is modified with an oligonucleotide chain sS1, and after reaction, the obtained working electrode is HCR / rS1 / MCH / H1 / Au-coated HPC / substrate. According to the sensor for detecting cadmium, the sensor is prepared by combining Cd < 2 + >-dependent deoxyribonuclease (DNAzyme), hybrid chain amplification reaction (HCR) and hierarchical porous carbon (HPC). Obvious advantages are shown in key performances such as sensitivity and detection range. The method is used for detecting national standard substances of rice and soil, the result is well within a marked range, the adding standard recovery rate ranges from 98.4% to 107.0%, the method has good consistency with the detection result of an atomic absorption graphite furnace (AAS) method, and the method has good application value in ultrasensitive accurate analysis of Cd < 2 + > in the fields of food, environment, sanitation and the like.

Some embodiments of the present disclosure relate to one or more compositions that upregulate the production of one or more sequences of mRNA. The sequences of mRNA may encode for translation of a target biomolecule, thereby causing an increase in bioavailability of the target biomolecule within a subject that is administered the one or more compositions. In some embodiments of the present disclosure, the target biomolecule is a fusion protein with an Fc fragment, such as a toll-like receptor 3-Fc (TLR3-Fc). In some embodiments of the present disclosure, the target biomolecule is toll-like receptor 9-Fc (TLR9-Fc). In some embodiments of the present disclosure, the target biomolecule is deoxyribonuclease I-Fc (DNAse I-Fc). In some embodiments of the present disclosure, the target biomolecule is neural growth factor-Fc (NGF-Fc). In some embodiments of the present disclosure, the target biomolecule is insulin-Fc.

Some embodiments of the present disclosure relate to one or more compositions that upregulate the production of one or more sequences of mRNA. The sequences of mRNA may encode for translation of a target biomolecule, thereby causing an increase in bioavailability of the target biomolecule within a subject that is administered the one or more compositions. In some embodiments of the present disclosure, the target biomolecule is a fusion protein with an Fc fragment, such as a toll-like receptor 3-Fc (TLR3-Fc). In some embodiments of the present disclosure, the target biomolecule is toll-like receptor 9-Fc (TLR9-Fc). In some embodiments of the present disclosure, the target biomolecule is deoxyribonuclease I-Fc (DNAse I-Fc). In some embodiments of the present disclosure, the target biomolecule is neural growth factor-Fc (NGF-Fc). In some embodiments of the present disclosure, the target biomolecule is insulin-Fc.

The application discloses a kind of ternary co-modified engineering velvet MSCs exosomes and preparation method and application thereof, belong to the biomedicine technical field, it includes velvet MSCs exosomes, polyarginine polypeptide-deoxyribonuclease I complex located on the surface of velvet MSCs exosome, and RGD polypeptide with Arg-Gly-Asp sequence modified on the surface of velvet MSCs exosome.The purpose is to solve the problem that the "integration" treatment mode of multiple pathological links cannot be realized when existing RA is treated, for the first time, three active components polyarginine polypeptide (P-Arg), deoxyribonuclease I (Dnase I) and RGD polypeptide are modified on the surface of velvet MSCs exosome, to form surface engineered modified exosome complex, multiple functions of immune regulation, cfDNA removal, targeted delivery and tissue protection can be realized in RA lesion, and a new, systematic treatment method is provided for rheumatoid arthritis.

This application belongs to the field of bioanalytical detection technology, specifically disclosing a detection system and method for live Salmonella based on deoxyribonuclease activation. The detection system includes a Sub probe, a deoxyribonuclease probe, a hairpin probe, hyperbranched dendritic nanomolecules, a crRNA / Cas12a binary complex, a fluorescent probe, and magnetic beads. The hyperbranched dendritic nanomolecules include substrate A, substrate B, and a trigger strand B probe. Substrate A consists of an A-F strand probe, an A-Q strand probe, and a helper strand A probe, while substrate B consists of a B-F strand probe, a B-Q strand probe, and a helper strand B probe. This detection system specifically recognizes ribonuclease H2 released only by the metabolism of live Salmonella and utilizes it to activate the Sub-Dz substrate, ensuring from the source that only live bacteria can trigger the subsequent reaction. This effectively solves the problem of interference from dead bacteria, achieving highly sensitive, highly specific, and rapid detection of live Salmonella.