Obligate halophile fast culture method

A fast technology for halophilic bacteria, applied in the field of water treatment technology and biological culture, can solve problems such as high-salt organic wastewater treatment problems, achieve the effects of shortening domestication and commissioning time, improving efficiency, and enhancing processing capacity

Inactive Publication Date: 2019-05-28
SHANGHAI LEZE ENVIRONMENTAL ENG
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Problems solved by technology

[0016] The invention provides a rapid culture method for halophilic bacteria to solve the problem of high-salt organic wastewater treatment

Method used

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  • Obligate halophile fast culture method

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Experimental program
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Effect test

Embodiment 1

[0035] Cultivate halophiles as follows:

[0036] (1) Configure trace element solution, which contains the following components: MnSO 4 ·7H 2 O 0.01g / L, ZnSO 4 ·7H 2O 0.05 g / L, Na 2 MoO 4 0.01 g / L, AlK(SO 4 ) 2 0.01 g / L, H 2 BO 4 0.01 g / L, CaCl 2 2H 2 O 0.01 g / L, CoCl 2 ·6H 2 O 0.2 g / L.

[0037] Prepare vitamin solution, containing the following ingredients: cobalamin 0.01 g / L, ascorbic acid 0.025 g / L, riboflavin 0.025 g / L, citric acid 0.02 g / L, pyridoxal 0.05 g / L, creatine 0.025 g / L L.

[0038] To prepare the basal medium of halophilic bacteria, add methanol 1.42 g, glucose 3 g, MgSO 4 ·7H 2 O 0.5g, NaCl 20g, (NH 4 ) 2 SO 4 2 g, KH 2 PO 4 0.02 g, take 1ml of trace elements and 1ml of vitamin solution, mix the above ingredients with distilled water to 1000mL, and the pH value is 6~8.

[0039] Prepare an enrichment medium containing the following components: beef extract 3 g / L, peptone 5 g / L, NaCl 20 g / L, KH 2 PO 4 0.02 g / L, agar 20 g / L, prepared wi...

Embodiment 2

[0050] Cultivate halophiles as follows:

[0051] (1) Configure the trace element solution as an aqueous solution with the following composition: MnSO 4 ·7H 2 O 0.01g / L, ZnSO 4 ·7H 2 O 0.05g / L, Na 2 MoO 4 0.01 g / L, AlK(SO 4 ) 2 0.01 g / L, H 2 BO 4 0.01 g / L, CaCl 2 2H 2 O 0.01 g / L, CoCl 2 ·6H 2 O 0.2 g / L.

[0052] Prepare vitamin solution as an aqueous solution with the following components: 0.01 g / L cobalamin, 0.025 g / L ascorbic acid, 0.025 g / L riboflavin, 0.02 g / L citric acid, 0.05 g / L pyridoxal, and 0.025 g creatine / L.

[0053] To prepare the basal medium of halophilic bacteria, add methanol 2 g / L, glucose 4 g / L, MgSO 4 ·7H 2 O 0.7g / L, NaCl 22 g / L, (NH 4 ) 2 SO 4 3 g / L, KH 2 PO 4 0.01~0.03 g / L, take 1ml / L of trace elements, 1ml / L of vitamin solution and distilled water, the pH value is 6~8.

[0054] Prepare an enrichment medium containing the following components: beef extract 3 g / L, peptone 5 g / L, NaCl 20 g / L, KH 2 PO 4 0.02 g / L, agar 20 g / L, pr...

Embodiment 3

[0064] (1) Configure the trace element solution as an aqueous solution with the following composition: MnSO 4 ·7H 2 O 0.01g / L, ZnSO 4 ·7H 2 O 0.05g / L, Na 2 MoO 4 0.01 g / L, AlK(SO 4 ) 2 0.01 g / L, H 2 BO 4 0.01 g / L, CaCl 2 2H 2 O 0.01 g / L, CoCl 2 ·6H 2 O 0.2 g / L.

[0065] Prepare vitamin solution as an aqueous solution with the following components: 0.01 g / L cobalamin, 0.025 g / L ascorbic acid, 0.025 g / L riboflavin, 0.02 g / L citric acid, 0.05 g / L pyridoxal, and 0.025 g creatine / L.

[0066] To prepare the basal medium of halophilic bacteria, add methanol 1.00 g, glucose 3 g, MgSO 4 ·7H 2 O 0.2g, NaCl 17g, (NH 4 ) 2 SO 4 1 g, KH 2 PO 4 0.01 g, take 1ml of trace elements, 1ml of vitamin solution, mix with distilled water to 1000mL, pH value is 6~8.

[0067] Prepare an enriched medium containing the following components: beef extract 4g / L, peptone 7g / L, NaCl 25g / L, KH 2 PO 4 0.03 g / L, agar 25 g / L, prepared with distilled water, the pH value is 7.4, take 1...

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Abstract

The invention discloses an obligate halophile fast culture method. A basic culture medium contains MnSO4.7H2O, ZnSO4.7H2O, Na2MoO4, AlK(SO4)2, H2BO4, CaCl2.2H2O and CoCl2.6H2O; an enriching culture medium contains beef extracts, peptone, NaCl, KH2PO4, agaragar and distilled water, and the pH value is 7.2 to 7.4. The phosphate buffer solution contains NaCl, KCl, Na2HPO4 and KH2PO4, and the pH valueis 7.2 to 7.4. The sludge obtained from a secondary sedimentation tank in a sewage plant is subjected to aerobic culture in the basic culture medium; the cultured sludge is subjected to enrichment culture by the enriching culture medium in a flat plate coating mode, and is put into a biomedical culture box to be cultured for 72h; the enriching culture medium is taken; surface layer sludge is scraped and taken; the phosphate buffer solution is used for repeatedly flushing; then centrifugation is performed by a high-speed centrifugation machine; supernatant fluid is removed; bottom layer sludgeis taken; the sludge is high-concentration halophilic sludge. The high-concentration halophilic sludge and the ordinary sludge are taken and proportionally added into a culture medium to be cultured.The method is used for fast culturing obligate halophile, so that the practical problems of biological treatment of high-salt waste water is solved.

Description

technical field [0001] The invention belongs to the technical field of water treatment technology and biological culture, and relates to the cultivation and application of halophilic bacteria degrading high-salinity wastewater, in particular to a rapid cultivation method of halophilic bacteria. Background technique [0002] High-salt wastewater refers to wastewater with a salt content greater than 1.0% (mass fraction, the same below), mainly from some industrial production units. In addition to organic pollutants, these wastewater also contain a large amount of inorganic salts, such as Cl - , SO 4 2- 、Na + , Ca 2+ etc. The presence of these salts has obvious inhibitory effect on conventional biological treatment. [0003] Industrial wastewater in petroleum exploration, chemical industry, pharmaceutical and other industries not only contains oil, organic matter, heavy metals and other substances, but also contains a large amount of Cl - , SO 4 2- , Na+, Ca 2 + and oth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00
Inventor 张军付振华吕文玉
Owner SHANGHAI LEZE ENVIRONMENTAL ENG
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