Liquid fermentation tank inoculation device of obligate anaerobes, and inoculation method
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A fermenter and inoculation needle technology, applied in the field of microbial fermentation, can solve the problems of lack of anaerobic fermenter inoculation device, sensitivity, etc.
Pending Publication Date: 2018-12-07
吉态来博(北京)生物科技发展有限公司
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Due to the extreme sensitivity of this type of anaerobic bacteria to the presence of trace oxygen, its application is largely limited to the laboratory shake flask stage
The inoculation in the shake flask stage...
Method used
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Embodiment 1
[0113] Such as figure 1 Make inoculation device as shown, this inoculation device comprises threaded mouth triangular flask 14 and hollow bottle cap 5, passes into a stainless steel two-way 15 in hollow bottle cap 5, and sealing gasket 11 is arranged in threaded bottleneck 14 and bottle cap 5.
[0114] Two valves are housed on the inflow pipe 4 of the stainless steel two-way 15, one inflow pipe main valve 3, one inflow pipe purge valve 2. The air inlet is connected with the stainless steel inflow pipe 4 with a flexible pipe 10, and connected to the microporous membrane filter 1, and the microporous membrane filter 1 is connected with a section of flexible pipe as the air inlet 13 during deoxygenation.
[0115] Stainless steel two-way 15 outflow pipe 8 is equipped with two valves, one outflow pipe main valve 6, one outflow pipe purge valve 7;
Embodiment 2
[0117] Taking a 50L fermenter as an example, Moorella thermoacetica, ATCC49707 is used for fermentation, and syngas is used as the gas raw material to produce acetic acid.
[0118] The composition content of the synthesis gas is shown in Table 1 below.
[0119] Table 1: Contents of each component of synthesis gas
[0122] In fermentor, feed steam and maintain pressure at 0.1MPa to sterilize for 30 minutes, then pass the gas in Table 1 into fermentor, insert bacterial classification Moorella thermoacetica (ATCC 49707 ) (http: / / www.atcc.org), the reaction temperature is controlled at 60°C, the pH is 7.0,...
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Abstract
The invention relates to a liquid fermentation tank inoculation device of obligate anaerobes, and an inoculation method, and more specifically relates to a gas flowing pipeline. The liquid fermentation tank inoculation device of obligate anaerobes comprises 1, a double-way plug provided with two through holes; 2, a flow inlet pipe which is designed to pass through one of the two through holes, wherein the external wall of the flow inlet pipe is connected with the through hole through gas tightness connection, the flow inlet pipe is provided with a flow inlet pipe main valve; and 3, a flow outlet pipe designed to pass through the other through hole, wherein the external wall of the flow outlet pipe is connected with the through hole through gas tightness connection, and the flow outlet pipeis provided with a flow outlet pipe main valve; the flow inlet pipe is provided with a branch pipe in front of the flow inlet pipe main valve, the branch pipe is provided with a flow inlet pipe blow-down valve; the flow outlet pipe main valve is provided with a branch pipe in front of the flow outlet main valve, and the branch pipe is provided with a flow outlet pipe blow-down valve. The liquid fermentation tank inoculation device contains the gas flowing pipeline.
Description
technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to an inoculation device and an inoculation method for a liquid fermentation tank of obligate anaerobic bacteria. Background technique [0002] With the continuous advancement of technology, the application of obligate anaerobic bacteria in fermentation production has gradually attracted people's attention, such as the process of using anaerobic bacteria to produce hydrogen, and the process of using acetic acid bacteria to produce acetic acid, etc. of favor. Because such anaerobic bacteria are extremely sensitive to the presence of trace oxygen, their application is largely limited to the laboratory shake flask stage. The inoculation in the shake flask stage can be carried out in an anaerobic box, but once it enters the industrial production stage, there is currently a lack of suitable anaerobic fermentation tank inoculation devices and corresponding inoculation me...
Claims
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