Obligate citrus AM inoculant and preparation method thereof

A technology of inoculant and fungal inoculant, applied in the field of citrus obligate AM inoculant and its preparation, which can solve the problems of poor effect, low content of arbuscular mycorrhizal fungi, and difficulty in large-scale commercial production of AM fungal inoculant

Inactive Publication Date: 2018-08-17
广西青又青生物肥业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, the industrial and commercial production of AM fungal inoculants is still in its infancy in China. In addition, AM fungi cannot be cultured independently of the host plant, so it is still difficult to achieve large-scale commercial production of AM fungal inoculants
[0005] At this stage, various improvements have been made to the production process of AM fungal inoculants. For example, patent CN02159722.7 discloses a method for producing arbuscular mycorrhizal fungal inoculants. 30-45 parts of perlite, 15-25 (weight) parts of zeolite on the culture medium, inoculate the bacterial agent that contains the propagule of arbuscular mycorrhizal fungus that is equivalent to 5-10% of the volume of the matrix, and adopts multiple hosts Plants are cultivated in mixed sowing and mixed cropping mode; the nutrient solution is p

Method used

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  • Obligate citrus AM inoculant and preparation method thereof
  • Obligate citrus AM inoculant and preparation method thereof
  • Obligate citrus AM inoculant and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A citrus obligate AM fungal inoculant prepared by the following method:

[0046] (1) Get 1 kg of sterile Hovenia citrus seeds as citrus rootstock seeds, wash the aseptic citrus rootstock seeds with 54.5° C. sterile warm water, then soak them in sterile warm water for 2 hours, rinse and dry with 75% alcohol;

[0047] (2) Mix citrus obligate AM fungi with sterile soil at a ratio of 1:900, cultivate dried citrus rootstock seeds in it, and carry out citrus rootstock seedling cultivation, so that citrus obligate AM fungi can be fully multiplied and combined with citrus The roots of the rootstock are fully symbiotic;

[0048] (3) citrus rootstock seedlings were raised for 14 weeks, and finally the citrus rootstock root system and its culture soil were pulverized to make the AM extraroot mycelium, spores and other propagules in the soil; Raw AM mycorrhizal fungus, mixed evenly and packaged to obtain citrus obligate AM fungal inoculant;

[0049] (4) Control the moisture conte...

Embodiment 2

[0053] A citrus obligate AM fungal inoculant prepared by the following method:

[0054] (1) Get 1 kg of sterile Hovenia citrus seeds as citrus rootstock seeds, wash the sterile citrus rootstock seeds with sterile warm water at 30°C, then soak them in sterile warm water for 0.5h, rinse and dry with 65% alcohol;

[0055] (2) Mix citrus obligate AM fungi with sterile soil at a ratio of 1:800, cultivate dried citrus rootstock seeds in it, and carry out citrus rootstock seedling cultivation, so that citrus obligate AM fungi can be fully multiplied and combined with citrus The roots of the rootstock are fully symbiotic;

[0056] (3) citrus rootstock seedlings were raised for 12 weeks, and finally the citrus rootstock root system and its culture soil were pulverized to make the AM extraroot mycelium, spores and other propagules in the soil; Raw AM mycorrhizal fungus, mixed evenly and packaged to obtain citrus obligate AM fungal inoculant;

[0057] (4) Control the water content of t...

Embodiment 3

[0061] A citrus obligate AM fungal inoculant prepared by the following method:

[0062] (1) Get 1 kg of sterile Hovenia citrus seeds as citrus rootstock seeds, wash the sterile citrus rootstock seeds with 70°C sterile warm water, then soak them in sterile warm water for 4 hours, rinse and dry with 85% alcohol;

[0063] (2) Mix citrus obligate AM fungi with sterile soil at a ratio of 1:1000, cultivate dried citrus rootstock seeds in it, and carry out citrus rootstock seedling cultivation, so that citrus obligate AM fungi can be fully multiplied and combined with citrus The roots of the rootstock are fully symbiotic;

[0064] (3) citrus rootstock seedlings were raised for 16 weeks, and finally the citrus rootstock root system and its culture soil were pulverized to make the AM exogenous mycelium, spores and other propagules in the soil; Raw AM mycorrhizal fungus, mixed evenly and packaged to obtain citrus obligate AM fungal inoculant;

[0065] (4) Control the water content of ...

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Abstract

The invention relates to the technical field of agricultural plant and especially relates to an obligate citrus AM inoculant and a preparation method thereof. According to the obligate citrus AM inoculant disclosed by the invention, obligate citrus AM fungi obtained from separation is mixed with sterile soil according to a proportion of 1 to (800 to 1000), citrus rootstock seedling growing is performed in the citrus AM fungi and the sterile soil for 12 to 16 weeks, the obligate citrus AM fungi are fully propagated and achieve full mutualism with citrus rootstock root systems, and the citrus rootstock root systems and cultivation soil of the citrus rootstock root systems are smashed to be packaged to obtain the obligate citrus AM inoculant. According to the obligate citrus AM inoculant disclosed by the invention, a mixing proportion of the obligate citrus AM fungi and the sterile soil is strictly controlled; furthermore, citrus rootstock seedling growing time is controlled; finally, theobligate citrus AM fungi inoculant which has high AM fungi content, good inoculation activity and appropriate proportion of mycelium outside roots to spore to mycelium inside the roots to endogenousAM mycorrhiza fungi can be obtained.

Description

technical field [0001] The invention relates to the technical field of agricultural planting, in particular to a citrus obligate AM inoculant and a preparation method thereof. Background technique [0002] AM fungi, namely arbuscular mycorrhizal fungi, are an important class of soil microorganisms in agricultural ecosystems. They have many beneficial effects on crop growth and development, improvement of nutrient absorption, and improvement of disease resistance and stress resistance. [0003] Arbuscular mycorrhiza, in 1885, the German plant physiologist and forest scientist Frank first coined the term "mycorrhizal" (Fungus-root or Mycorrhiza). Mycorrhizae are a common plant symbiosis phenomenon in nature, which is a combination of mycorrhizal fungal hyphae in soil and vegetative roots of higher plants. The symbiotic fungi obtain the necessary carbohydrates and other nutrients from the plants, and the plants also obtain the required nutrients and water from the fungi, so as...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 赵文青苏善高陈文艳
Owner 广西青又青生物肥业有限公司
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