Obligate citrus AM inoculant and preparation method thereof

Abstract

The invention relates to the technical field of agricultural plant and especially relates to an obligate citrus AM inoculant and a preparation method thereof. According to the obligate citrus AM inoculant disclosed by the invention, obligate citrus AM fungi obtained from separation is mixed with sterile soil according to a proportion of 1 to (800 to 1000), citrus rootstock seedling growing is performed in the citrus AM fungi and the sterile soil for 12 to 16 weeks, the obligate citrus AM fungi are fully propagated and achieve full mutualism with citrus rootstock root systems, and the citrus rootstock root systems and cultivation soil of the citrus rootstock root systems are smashed to be packaged to obtain the obligate citrus AM inoculant. According to the obligate citrus AM inoculant disclosed by the invention, a mixing proportion of the obligate citrus AM fungi and the sterile soil is strictly controlled; furthermore, citrus rootstock seedling growing time is controlled; finally, theobligate citrus AM fungi inoculant which has high AM fungi content, good inoculation activity and appropriate proportion of mycelium outside roots to spore to mycelium inside the roots to endogenousAM mycorrhiza fungi can be obtained.

Description

technical field [0001] The invention relates to the technical field of agricultural planting, in particular to a citrus obligate AM inoculant and a preparation method thereof. Background technique [0002] AM fungi, namely arbuscular mycorrhizal fungi, are an important class of soil microorganisms in agricultural ecosystems. They have many beneficial effects on crop growth and development, improvement of nutrient absorption, and improvement of disease resistance and stress resistance. [0003] Arbuscular mycorrhiza, in 1885, the German plant physiologist and forest scientist Frank first coined the term "mycorrhizal" (Fungus-root or Mycorrhiza). Mycorrhizae are a common plant symbiosis phenomenon in nature, which is a combination of mycorrhizal fungal hyphae in soil and vegetative roots of higher plants. The symbiotic fungi obtain the necessary carbohydrates and other nutrients from the plants, and the plants also obtain the required nutrients and water from the fungi, so as...

AI Technical Summary

Problems solved by technology

Generally speaking, the industrial and commercial production of AM fungal inoculants is still in its infancy in China. In addition, AM fungi cannot be cultured independently of the host plant, so it is still difficult to achieve large-scale commercial production of AM fungal inoculants

[0005] At this stage, various improvements have been made to the production process of AM fungal inoculants. For example, patent CN02159722.7 discloses a method for producing arbuscular mycorrhizal fungal inoculants. 30-45 parts of perlite, 15-25 (weight) parts of zeolite on the culture medium, inoculate the bacterial agent that contains the propagule of arbuscular mycorrhizal fungus that is equivalent to 5-10% of the volume of the matrix, and adopts multiple hosts Plants are cultivated in mixed sowing and mixed cropping mode; the nutrient solution is poured every 2 to 3 days; after 17 to 18 weeks of cultivation, the culture substrate and plant roots are collected; for example, patent application CN201511023372.7 discloses an arbuscular mycorrhizal fungus The agent is prepared according to the following steps: using Trifolium pratense seeds and Ophiopogon japonicus seeds as host plants, using Glomus moses, Glomus reticulum, Glomus teliotus or Glomus intraradices as inoculants, in a specific Cultivate under nutrient conditions, remove the parts above the root of the plant that has grown to the harvest period, and pulverize and mix the remaining root system and matrix to obtain the arbuscular mycorrhizal fungus inoculum. Both of these two inoculums contain various AM fungi. Less effective citrus obligate arbuscular mycorrhizal fungi when used in citrus cultivation are less effective

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